FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995855
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCGTCGTCACTCAGCTTGTTCATTTGCAGATAGA44440.4462497050273383No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30880.310085303583353No Hit
GTGTATTACTGTGCGAAAGATCATGACTGGAACTTTTCTGTCTGGGGCCA25500.25606137439687504No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTC23550.23648021047240814No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21430.21519197071862872No Hit
GTATTACTGTCAGCAGTTTGCTAACTCACCCATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCCACCGAA21330.21418780846609195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20220.20304160746293384No Hit
GTGTATTACTGTCAGCAGTTTGCTAACTCACCCATGTGCAGTTTTGGCCA19210.19289956871231254No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18430.18506710314252578No Hit
CAGTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17440.17512589684241178No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA15830.1589588845765699No Hit
CTCCTGTGCAGCCTCTGGATTCACCATTAGCACCTACACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG15530.15594639781895958No Hit
CAGTAATACACGGCCGCGTCGTCGTCACTCAGCTTGTTCATTTGCAGATA15330.15393807331388606No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15270.153335575962364No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15230.1529339110613493No Hit
GTAATACACTGCGAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14720.1478126835734118No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14280.14339436966225003No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14210.1426914560854743No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13380.13435690938941913No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12930.1298381792530037No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12660.12712694117115445No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12260.12311029216100738No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12180.12230696235897796No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC12110.12160404878220221No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC12030.12080071898017282No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT11800.11849114579933827No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT11510.11557907526698163No Hit
GGGTTCCCTGGCCCCAGACAGAAAAGTTCCAGTCATGATCTTTCGCACAGTAATACACGGCCGCGTCGTCGTCA11500.11547865904172797No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11150.11196409115784929No Hit
GAGTTTGGGCTGAGCTGGCTTTTCCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTTTTGGAGT11040.11085951268005885No Hit
CTCCTGCAGGGCCAGTCAGAATGTTGGCAGCAGCCACTTAGCCTGGTACC11030.11075909645480518No Hit
GTATTACTGTGCGAAAGATCATGACTGGAACTTTTCTGTCTGGGGCCAGG10980.1102570153285368No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC10870.10915243685074634No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10840.10885118817498532No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10660.10704369612041914No Hit
GGTCTGTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGCCTCACCCA10650.10694327989516546No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC10400.10443287426382354No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGACAGAAAAGTTCCAGTCATGATCTTTCGCACAGTAATA10330.10372996068704782No Hit
CCATTAGCACCTACACCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGCGATGGGTCTCGTCTGTAAATA10280.10322787956077942No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10250.1029266308850184No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10210.1025249659840037No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT10170.10212330108298898No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT10030.10071747392943753No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC10000.10041622525367648No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT9970.10011497657791545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGC200.006435824653.53356663
GTGGTAT1850.053.4712871
TCGACCA200.006630699653.1313559
CGACACT200.007694619751.16906416
TCTGGAG14450.048.2550470
GGTATCA7450.044.870841
TGGTATC2300.044.4814262
TATCGCA350.001124438740.9079164
GTATCAA20650.038.9839971
TATCAAC22750.035.0767822
GCTTACC400.002744602334.1109921
ATCAACG24100.033.5263063
AACGCAG24650.032.500016
GCAGATA14550.031.66254442
ATTTGCA14850.031.53601338
TTTGCAG14950.031.37280839
TGTTAGA5600.031.1288170
TCATTTG14900.030.90058336
TTGCAGA15150.030.77321640
AAATATC450.004741245430.50789835
Produced by FastQC (version 0.11.5)