FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532584
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24240.4551394709566942No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23760.44612680816547245No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18390.3452976431886801No Hit
GAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATTTTGCAACTTA18350.3445465879560783No Hit
CCCCTAAACTCCTGATCTATGAGGCGTCTAATTTAGAGACTGGGGTCCCA18130.3404157841767683No Hit
GAGTATTAGTAATTACTTGGCCTGGTATCAGCAGAAACCGGGGAACGCCCCTAAACTCCTGATCTATGAGGCGT18100.33985249275231705No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16710.31375332341940426No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15410.2893440283598456No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15340.2880296817027924No Hit
CCTCCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAATTA15030.28220900365012846No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14690.27582503417301313No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14440.27113093896925183No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14020.26324485902693284No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13580.25498325146831297No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13000.2440929505955868No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12750.2393988553918255No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12640.23733345350217058No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12220.22944737355985162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12150.2281330269027984No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAATTACTTGGCCTGGTATCAGCAGAAA12100.22719420786204617No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11630.21836930887897493No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCTAGCCGTGTCC11470.21536508794856773No Hit
GAGTGAATTCTGTGCCAGATCCACCGGCGGTGAATCTCGATGGGACCCCA11450.21498956033226685No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11340.21292415844261187No Hit
CTATGATTCTTACGTCACCTTCGGCCAAGGGACACGGCTAGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT11130.2089811184714524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10760.2020338575698857No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10370.1947110690520181No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10200.19151908431346043No Hit
GTTTAGGGGCGTTCCCCGGTTTCTGCTGATACCAGGCCAAGTAATTACTA9750.1830697129466901No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA9640.18100431105703513No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9630.18081654724888468No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9580.17987772820813244No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9420.17687350727772522No Hit
CTCCTGATCTATGAGGCGTCTAATTTAGAGACTGGGGTCCCATCGAGATT9320.1749958691962207No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8940.1678608444865035No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8600.16147687500938818No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8580.1611013473930873No Hit
GAATCATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATTCTGTG8460.15884818169528186No Hit
CGGCTAGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT8310.15603172457302508No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8160.15321526745076833No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8110.15227644841001609No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTCCCACC8100.15208868460186561No Hit
AATTTAGAGACTGGGGTCCCATCGAGATTCACCGCCGGTGGATCTGGCACAGAATTCACTCTCACCATCGGCAGC8030.15077433794481246No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7950.14927222747960886No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA7930.14889669986330795No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7890.14814564463070612No Hit
ACTTATTACTGCCAACACTATGATTCTTACGTCACCTTCGGCCAAGGGAC7760.14570471512475028No Hit
GTGCAGCCACAGTTCGTTTAATCTCTAGCCGTGTCCCTTGGCCGAAGGTG7600.14270049419434305No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7490.1406350923046881No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7470.14025956468838718No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7420.1393207456476349No Hit
GTATTAGTAATTACTTGGCCTGGTATCAGCAGAAACCGGGGAACGCCCCT7410.13913298183948447No Hit
GATCAGGAGTTTAGGGGCGTTCCCCGGTTTCTGCTGATACCAGGCCAAGT7270.13650428852537816No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7080.13293677617051958No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7050.13237348474606822No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7050.13237348474606822No Hit
TCTATGAGGCGTCTAATTTAGAGACTGGGGTCCCATCGAGATTCACCGCCGGTGGATCTGGCACAGAATTCACT7040.13218572093791778No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7020.13181019332161686No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6980.13105913808901506No Hit
GGTATCAGCAGAAACCGGGGAACGCCCCTAAACTCCTGATCTATGAGGCGTCTAATTTAGAGACTGGGGTCCCAT6820.12805491715860784No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6810.1278671533504574No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6790.12749162573415648No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6580.123548585762997No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6480.1216709476814925No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6460.12129542006519159No Hit
GTCTCCTCCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT6390.11998107340813843No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6350.11923001817553663No Hit
CACTATGATTCTTACGTCACCTTCGGCCAAGGGACACGGCTAGAGATTAAACGAACTGTGGCTGCACCAT6330.11885449055923573No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6250.11735238009403211No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6160.11566250582067804No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6130.1150992143962267No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA6120.11491145058807625No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6120.11491145058807625No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6070.113972631547324No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6000.11265828489027083No Hit
CTCTAGCCGTGTCCCTTGGCCGAAGGTGACGTAAGAATCATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGG6000.11265828489027083No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA5990.11247052108212037No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5890.11059288300061586No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC5850.10984182776801407No Hit
CCTCATAGATCAGGAGTTTAGGGGCGTTCCCCGGTTTCTGCTGATACCAGGCCAAGTAATTACTAATACTCTGA5790.10871524491911135No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC5590.10495996875610233No Hit
GTCTAATTTAGAGACTGGGGTCCCATCGAGATTCACCGCCGGTGGATCTGGCACAGAATTCACTCTCACCATCGG5580.10477220494795186No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5540.10402114971535008No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC5500.10327009448274826No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5450.10233127544199601No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC5450.10233127544199601No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5440.10214351163384555No Hit
GCTAGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC5340.10026587355234104No Hit
GCTCAACACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCTG5330.1000781097441906No Hit
GTCTCCTACAGATGCAGACAGGGTGGGAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG5330.1000781097441906No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC200.00817143750.393421
GTGGTAT1050.041.594571
TACTGTT650.001072748141.29027670
CGTATGC1500.039.1298141
TCGTATG1500.039.03185340
GATCTCG1400.038.93622636
ATCTCGT1500.036.4133137
CTTATAC1700.035.5718231
ACGAGAC1700.035.57182322
GTATGCC1800.034.6232242
CACGAGA1650.034.61041321
CCCACGA1650.034.61041319
GTATCAA11900.034.4425621
ATGCCGT1750.033.86343844
TCTGCAC502.1790205E-433.5829963
CTGCTTG1850.032.93821354
AGACCGA1750.032.6694925
GAGACCG1750.032.6572124
TCTCCGA1750.032.62348611
ACGAACC450.003947905331.66821752
Produced by FastQC (version 0.11.5)