FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532584
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25100.47128715845763297No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21960.4123293226983913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18890.3546858335962027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18740.3518693764739459No Hit
GAGTATTAGTAATTACTTGGCCTGGTATCAGCAGAAACCGGGGAACGCCCCTAAACTCCTGATCTATGAGGCGT17750.3332807594670512No Hit
CCCCTAAACTCCTGATCTATGAGGCGTCTAATTTAGAGACTGGGGTCCCA16950.3182596548150151No Hit
GAATTCACTCTCACCATCGGCAGCCTGCAGCCTGATGATTTTGCAACTTA16650.31262674057050155No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15780.29629128926141224No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15620.29328706833100504No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14820.27826596367896894No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14360.2696288285040482No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14150.2656857885328887No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13900.2609916933291274No Hit
CCTCCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAATTA13840.2598651104802247No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12550.23564357922881649No Hit
GAGTGAATTCTGTGCCAGATCCACCGGCGGTGAATCTCGATGGGACCCCA11940.22418998693163897No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11800.22156129361753266No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11580.2174304898382227No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11470.21536508794856773No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11290.2119853394018596No Hit
CTATGATTCTTACGTCACCTTCGGCCAAGGGACACGGCTAGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT10890.20447478707584155No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAATTACTTGGCCTGGTATCAGCAGAAA10830.20334820422693886No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10820.2031604404187884No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10650.19996845568023072No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10640.19978069187208025No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA10400.19527436047646943No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10190.19133132050530996No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCTAGCCGTGTCC10120.1900169738482568No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10060.1888903909993541No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10050.18870262719120365No Hit
GTTTAGGGGCGTTCCCCGGTTTCTGCTGATACCAGGCCAAGTAATTACTA9680.18175536628963695No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9570.17968996439998197No Hit
CTCCTGATCTATGAGGCGTCTAATTTAGAGACTGGGGTCCCATCGAGATT9090.1706773016087603No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9070.1703017739924594No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9010.1691751911435567No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8610.16166463881753865No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8460.15884818169528186No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8150.15302750364261786No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8050.15114986556111337No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTCCCACC7980.1498355189040602No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7920.1487089360551575No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7890.14814564463070612No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7820.146831297973653No Hit
CGGCTAGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT7760.14570471512475028No Hit
ACTTATTACTGCCAACACTATGATTCTTACGTCACCTTCGGCCAAGGGAC7620.14307602181064394No Hit
GAATCATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGAATTCTGTG7440.13969627326393583No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7320.13744310756613043No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7280.1366920523335286No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA7180.1348144142520241No Hit
GATCAGGAGTTTAGGGGCGTTCCCCGGTTTCTGCTGATACCAGGCCAAGT7180.1348144142520241No Hit
AATTTAGAGACTGGGGTCCCATCGAGATTCACCGCCGGTGGATCTGGCACAGAATTCACTCTCACCATCGGCAGC7110.13350006759497093No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7110.13350006759497093No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6970.13087137428086462No Hit
TCTATGAGGCGTCTAATTTAGAGACTGGGGTCCCATCGAGATTCACCGCCGGTGGATCTGGCACAGAATTCACT6930.1301203190482628No Hit
GGGTGGGAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG6840.12843044477490873No Hit
GTGCAGCCACAGTTCGTTTAATCTCTAGCCGTGTCCCTTGGCCGAAGGTG6830.12824268096675828No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6710.12598951526895288No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6690.125613987652652No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6570.12336082195484656No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6570.12336082195484656No Hit
GGTATCAGCAGAAACCGGGGAACGCCCCTAAACTCCTGATCTATGAGGCGTCTAATTTAGAGACTGGGGTCCCAT6500.1220464752977934No Hit
GTATTAGTAATTACTTGGCCTGGTATCAGCAGAAACCGGGGAACGCCCCT6480.1216709476814925No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6470.12148318387334203No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6460.12129542006519159No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6300.11829119913478436No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6250.11735238009403211No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6200.11641356105327985No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6120.11491145058807625No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6090.1143481591636249No Hit
CTCTAGCCGTGTCCCTTGGCCGAAGGTGACGTAAGAATCATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGG6080.11416039535547444No Hit
GTCTCCTCCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT5960.11190722965766903No Hit
CACTATGATTCTTACGTCACCTTCGGCCAAGGGACACGGCTAGAGATTAAACGAACTGTGGCTGCACCAT5920.11115617442506723No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCCGATGGTGAGAGTGA5640.10589878779685459No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC5610.10533549637240322No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5580.10477220494795186No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC5560.10439667733165096No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT5550.10420891352350052No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5520.10364562209904916No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5500.10327009448274826No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5500.10327009448274826No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5500.10327009448274826No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5490.10308233067459782No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5460.10251903925014645No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5410.1015802202093942No Hit
CCTCATAGATCAGGAGTTTAGGGGCGTTCCCCGGTTTCTGCTGATACCAGGCCAAGTAATTACTAATACTCTGA5390.1012046925930933No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5340.10026587355234104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG250.001265851880.6732270
AACCCGA150.002639153367.044618
TCCCGAA350.004805557.6237370
CTATACA200.00821174750.33071520
ATCCGAT200.00821785150.321261
GTGGTAT1400.045.5287551
TATTCTA901.1649502E-437.34871370
TTATGGT750.002146358635.85476770
TCTCGCG2358.185452E-1131.46827770
GTATCAA11700.030.6801971
AGTTGAG450.00531792529.7976025
AAAGCGC500.00568772129.39005961
TGTTAGA7550.029.38428570
GACAGGT1053.0535375E-828.7550051
TATCAAC13350.027.1242582
AACTTGT2255.8289515E-826.89107570
CTATACT757.488476E-526.8178424
GCGTAGA14050.026.79537670
ATCAACG13400.026.7652973
AACGCAG13700.026.426346
Produced by FastQC (version 0.11.5)