FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153817
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTATCCTCGGCGCTCAGGCTGTTCATTTGTAGAAACA72990.6325959835918521No Hit
GTATATTACTGTGCGAGAGATATGTTTGACTGGTTATCACAAAACAACTGGTTCGCCCCCTGGGGCCAGGGAACC53890.4670584676772833No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34640.3002209189152179No Hit
CCTATGAGCTGACTCAGCCACCCTCGGTGTCTGTGGCCCCAGGACAGACG29900.25913988093432494No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCTCTACTATTATCCCACAC29900.25913988093432494No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25280.21909886923142924No Hit
TACATATACTACGCGGACTCAGTGAAGGGCCGATTCACCGTCACCAGAGACAACGCCAAGAATACACTGTTTCTA25110.2176254986709331No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24540.21268537385044595No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCTCTA23650.20497184562196605No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22370.19387823198999493No Hit
GGATTACCTGTGGGGGAAACATCATTGGAAGTAATGGTGTGCACTGGTATCAGCAGAAGCCAGGCCAGGCCC21760.1885914317435087No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21660.18772474317851096No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20880.1809645723715286No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20550.17810450010703605No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20200.17507109012954394No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19940.17281769986054982No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGATTTAACATGAATTGGGTCCGCCAGGCTCCAGGAAAGGGG19670.1704776407350559No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19550.16943761445705863No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG17680.15323053829160085No Hit
GTATATGTAAGTACTACTACTAGTGATGGATGAGACCCACTCCAGCCCCTTTCCTGGAGCCTGGCGGACCCAATT17450.15123715459210602No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17440.15115048573560624No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17080.14803040690161437No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16820.14577701663262024No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16230.1406635540991336No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16210.14049021638613401No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAGCTGACT16060.1391901835386374No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16060.1391901835386374No Hit
CCATTACTTCCAATGATGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACAGACACCGAGGGTGG15720.1362434424176451No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15670.13581009813514622No Hit
CTGCACAGACTCTGTGACCTCCTATGAGCTGACTCAGCCACCCTCGGTGT15660.13572342927864645No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15620.13537675385264736No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15400.13347003900965232No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15310.13269001930115434No Hit
GGATAATAGTAGAGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC15070.13060996674515976No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC15010.13008995360616113No Hit
CCCCTGTGTTGGTCATCTATGATGATATCGACCGGCCCTCAGGGATCCCT14920.12930993389766315No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14710.1274898879111679No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14660.127056543628669No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14470.1254098353551733No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14330.12419647136417647No Hit
CTATTATCCCACACCTGACAATAATACCCGGCCTCATCCCCGGCTTCGAC14300.12393646479467715No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14180.12289643851667986No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAGCTGACTCAGC14120.1223764253776812No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14100.12220308766468166No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGATACCAGTGCACACCATTACTT13980.12116306138668437No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13980.12116306138668437No Hit
GCCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAACTCTGGGAACACGG13880.12029637282168662No Hit
CTCTGTGACCTCCTATGAGCTGACTCAGCCACCCTCGGTGTCTGTGGCCCCAGGACAGACGGCCAGGATTAC13870.12020970396518685No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA13750.11916967768718957No Hit
GTATTATTGTCAGGTGTGGGATAATAGTAGAGATCATGTGGTCTTCGGCG13740.1190830088306898No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13600.11786964483969295No Hit
CAGTAATATACAGCCGTATCCTCGGCGCTCAGGCTGTTCATTTGTAGAAA13480.11682961856169566No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13460.11665628084869611No Hit
CAATAATACCCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT13280.11509624143170016No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13260.11492290371870062No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13200.11440289057970197No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13200.11440289057970197No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13200.11440289057970197No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13140.11388287744070334No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13060.11318952658870515No Hit
GTCTGGGGGAGGCCTGGTCAAACCTGGGGGGTCCCTGAGACTCTCCTGTG13060.11318952658870515No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG12810.11102280517621078No Hit
GTGTTGGTCATCTATGATGATATCGACCGGCCCTCAGGGATCCCTGACCG12810.11102280517621078No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12650.10963610347221439No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGGGTAT12500.1083360706247178No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGGGGTTCCCTGG12490.10824940176821801No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12320.10677603120772185No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12240.10608268035572366No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12170.10547599836022523No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACATCATTGGAAGT12160.10538932950372545No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12110.10495598522122658No Hit
GCACAGACTCTGTGACCTCCTATGAGCTGACTCAGCCACCCTCGGTGTCT11950.10356928351723019No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC11680.1012292243917363No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGT11650.10096921782223697No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11650.10096921782223697No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11630.10079588010923743No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11570.10027586697023878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)