FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153817
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTATCCTCGGCGCTCAGGCTGTTCATTTGTAGAAACA68630.5948083621579505No Hit
GTATATTACTGTGCGAGAGATATGTTTGACTGGTTATCACAAAACAACTGGTTCGCCCCCTGGGGCCAGGGAACC50770.44001778444935374No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32680.2832338230412622No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCTCTACTATTATCCCACAC28330.24553287046386035No Hit
CCTATGAGCTGACTCAGCCACCCTCGGTGTCTGTGGCCCCAGGACAGACG26770.23201252884989562No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24950.21623879696693668No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23830.20653188503896197No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCTCTA23330.20219844221397326No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23030.19959837651898008No Hit
TACATATACTACGCGGACTCAGTGAAGGGCCGATTCACCGTCACCAGAGACAACGCCAAGAATACACTGTTTCTA22010.19075815315600309No Hit
GGATTACCTGTGGGGGAAACATCATTGGAAGTAATGGTGTGCACTGGTATCAGCAGAAGCCAGGCCAGGCCC21450.18590469719201572No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20020.173511050712548No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGATTTAACATGAATTGGGTCCGCCAGGCTCCAGGAAAGGGG19800.17160433586955295No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19650.17030430302205635No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19440.1684842570355611No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19420.16831091932256156No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19050.1651041716320699No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18950.16423748306707217No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18770.16267744365007622No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18460.1599907090985832No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17590.15245051858310288No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17230.14933043974911098No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17220.1492437708926112No Hit
GTATATGTAAGTACTACTACTAGTGATGGATGAGACCCACTCCAGCCCCTTTCCTGGAGCCTGGCGGACCCAATT16700.14473699035462295No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG16400.14213692465962974No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16110.1396235278211363No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16090.13945019010813672No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAGCTGACT16010.13875683925613855No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15420.13364337672265186No Hit
CCATTACTTCCAATGATGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACAGACACCGAGGGTGG15330.1328633570141539No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAGCTGACTCAGC15010.13008995360616113No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14980.12982994703666179No Hit
GGATAATAGTAGAGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14770.12800990105016652No Hit
CTGCACAGACTCTGTGACCTCCTATGAGCTGACTCAGCCACCCTCGGTGT14570.12627652392017105No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14570.12627652392017105No Hit
CTCTGTGACCTCCTATGAGCTGACTCAGCCACCCTCGGTGTCTGTGGCCCCAGGACAGACGGCCAGGATTAC14560.12618985506367128No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGGTCAGGGATCCCT14450.12523649764217376No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14220.12324311394267895No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14210.12315644508617918No Hit
GTATTATTGTCAGGTGTGGGATAATAGTAGAGATCATGTGGTCTTCGGCG14140.12254976309068076No Hit
CCCCTGTGTTGGTCATCTATGATGATATCGACCGGCCCTCAGGGATCCCT14100.12220308766468166No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC14050.12176974338218279No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13850.12003636625218729No Hit
CTATTATCCCACACCTGACAATAATACCCGGCCTCATCCCCGGCTTCGAC13810.11968969082618822No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13790.11951635311318867No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13430.11639627427919678No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13290.11518291028819994No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13270.11500957257520039No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13230.11466289714920129No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA13190.11431622172320222No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13150.11396954629720311No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12860.11145614945870966No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12860.11145614945870966No Hit
GTCTGGGGGAGGCCTGGTCAAACCTGGGGGGTCCCTGAGACTCTCCTGTG12610.10928942804621529No Hit
GCCCTCAGGGATCCCTGACCGATTCTCTGGCTCCAACTCTGGGAACACGG12570.10894275262021619No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12340.1069493689207214No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGATACCAGTGCACACCATTACTT12320.10677603120772185No Hit
CAATAATACCCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT12280.10642935578172276No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12040.10434930322572816No Hit
CAGTAATATACAGCCGTATCCTCGGCGCTCAGGCTGTTCATTTGTAGAAA11960.10365595237372997No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11950.10356928351723019No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11880.1029626015217318No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11860.10278926380873224No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11820.10244258838273315No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG11780.10209591295673405No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11680.1012292243917363No Hit
GTGTTGGTCATCTATGATGATATCGACCGGCCCTCAGGGATCCCTGACCG11630.10079588010923743No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)