FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496469
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37590.7571469719156685No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAATATGAGACCATTCAAAATGTCATCCCAAG32390.6524073003551077No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTG28340.5708312100050558No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21040.42379282492965326No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCT20510.4131174353282884No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCC19960.40203920083630595No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19870.400226398828527No Hit
ATAATAATCAGCCTCATCCTCAGATTGGAGGTCACTGATGGCCAGGGAGG17140.34523807125923267No Hit
GATTATTATTGTGCAGCTTGGGATGACATTTTGAATGGTCTCATATTCGG17100.3444323814779976No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17000.3424181570249099No Hit
GTCTCATATTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC16660.3355697938844117No Hit
ATATGAGACCATTCAAAATGTCATCCCAAGCTGCACAATAATAATCAGCC16140.32509582672835563No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15670.3156289717988434No Hit
CATTTATAGTGATGATCAGTGGCGCCCAGGGGTCCCTGACCGCTTCCTTGGCTCCAAGTCCGGCACCTCGGCCTC14850.29911233128352427No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAT14210.28622129478376296No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14050.2829985356588226No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13960.28118573365104366No Hit
CTCTTGCTCTGGGAGCAGCTCCAACATCGGAAGTCATTCTGTCAACTGGTACCAGTTAGTCCCAGGAACGGCCCC13080.26346055846387184No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13010.26205060134671043No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12700.2558065055421386No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12580.25338943619843335No Hit
GTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC12160.24492969349546498No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12050.24271404659706852No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12030.24231120170645093No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12020.2421097792611422No Hit
CTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTAACTGGTACCAGTTGACAGAATGACTTCCGATGTTGGAG11940.240498399698672No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11790.23747706301904045No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11520.23203865699570367No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11080.22317606940211776No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11080.22317606940211776No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11060.22277322451150022No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10730.21612628381631077No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10310.20766654111334243No Hit
CCTCCAATCTGAGGATGAGGCTGATTATTATTGTGCAGCTTGGGATGACA10190.20524947176963718No Hit
GACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAATATGAGACCATTCAAA9720.19578261684012496No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9540.1921570128245671No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9510.1915527454886408No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9390.18913567614493554No Hit
ACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAATATGAGACCATTCAAAA9340.1881285639183917No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9230.1859129170199952No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG9090.18309300278567242No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8890.179064553879497No Hit
GGCCATCAGTGACCTCCAATCTGAGGATGAGGCTGATTATTATTGTGCAGCTTGGGATGACATTTTGAATGGTCT8870.17866170898887945No Hit
GTACCAGTTAGTCCCAGGAACGGCCCCCAAACTCCTCATTTATAGTGATGATCAGTGGCGCCCAGGGGTCCCTGA8600.17322330296554267No Hit
CTGATGGCCAGGGAGGCCGAGGTGCCGGACTTGGAGCCAAGGAAGCGGTC8380.1687920091687497No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8370.16859058672344093No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8360.16838916427813216No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8260.16637493982504448No Hit
GGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGC8160.16436071537195676No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC8070.16254791336417782No Hit
GTCCGGCACCTCGGCCTCCCTGGCCATCAGTGACCTCCAATCTGAGGATG8030.16174222358294274No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT7950.1601308440204726No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAATATGAGAC7910.1593251542392375No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7900.15912373179392872No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7810.1573109297861498No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7720.15549812777837085No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7680.15469243799713575No Hit
AACTGGTACCAGTTGACAGAATGACTTCCGATGTTGGAGCTGCTCCCAGA7680.15469243799713575No Hit
AGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGG7630.15368532577059194No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7620.15348390332528317No Hit
GATTGGAGGTCACTGATGGCCAGGGAGGCCGAGGTGCCGGACTTGGAGCCAAGGAAGCGGTCAGGGACCCCTGGG7470.1504625666456516No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7330.1476426524113288No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7280.14663554018478497No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC7120.14341278105984462No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA7090.1428085137239183No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6910.13918290970836045No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6910.13918290970836045No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6880.13857864237243414No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6860.13817579748181658No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6760.1361615730287289No Hit
GATCAGTGGCGCCCAGGGGTCCCTGACCGCTTCCTTGGCTCCAAGTCCGG6600.13293881390378856No Hit
CCCCTGGGCGCCACTGATCATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTAACTGGTACCAGTTGA6520.13132743434131838No Hit
GTCCCTGACCGCTTCCTTGGCTCCAAGTCCGGCACCTCGGCCTCCCTGGC6410.12911178744292193No Hit
GTCAGGGACCCCTGGGCGCCACTGATCATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTAACTGGTA6360.12810467521637806No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC6330.12750040788045175No Hit
GTGATGATCAGTGGCGCCCAGGGGTCCCTGACCGCTTCCTTGGCTCCAAG6320.12729898543514298No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG6120.12327053652896756No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC6110.1230691140836588No Hit
GGCAGAGGGTCACCATCTCTTGCTCTGGGAGCAGCTCCAACATCGGAAGT6060.12206200185711495No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATTTATAGTGATGATCAGTGGCG6050.12186057941180617No Hit
GTGCCGGACTTGGAGCCAAGGAAGCGGTCAGGGACCCCTGGGCGCCACTG6030.12145773452118863No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC5980.12045062229464479No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC5970.120249199849336No Hit
GTTTGGGGGCCGTTCCTGGGACTAACTGGTACCAGTTGACAGAATGACTT5970.120249199849336No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC5960.12004777740402724No Hit
GATCATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTAACTGG5950.11984635495871847No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC5950.11984635495871847No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAG5890.11863782028686584No Hit
GCCCCCAAACTCCTCATTTATAGTGATGATCAGTGGCGCCCAGGGGTCCC5890.11863782028686584No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG5840.117630708060322No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGG5810.11702644072439568No Hit
GGGTCACCATCTCTTGCTCTGGGAGCAGCTCCAACATCGGAAGTCATTCT5730.11541506116192551No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC5670.1142065264900729No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5530.11138661225575011No Hit
GCTTGGTCCCCCCGCCGAATATGAGACCATTCAAAATGTCATCCCAAGCTGCACAATAATAATCAGCCTCATCCT5520.11118518981044134No Hit
GCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGCT5490.11058092247451504No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5370.10816385313080978No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT5370.10816385313080978No Hit
CCACTGATCATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTA5360.10796243068550102No Hit
CCCTGGCCATCAGTGACCTCCAATCTGAGGATGAGGCTGATTATTATTGT5330.10735816334957471No Hit
GAAATACACAGCCGTGTCGTCGGCTGTCAGGCTGTTCATTTGCAGAAACA5300.10675389601364837No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG5280.10635105112303085No Hit
GTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGGAGCAG5270.10614962867772208No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG5260.10594820623241331No Hit
GAGCAAGAGATGGTGACCCTCTGCCCGGGGGCCCCAGACGCTGAGGATGG5250.10574678378710455No Hit
GCTTGGGATGACATTTTGAATGGTCTCATATTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGG5250.10574678378710455No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5240.10554536134179575No Hit
GCTCCAACATCGGAAGTCATTCTGTCAACTGGTACCAGTTAGTCCCAGGA5170.10413540422463438No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA5150.10373255933401682No Hit
GCACAATAATAATCAGCCTCATCCTCAGATTGGAGGTCACTGATGGCCAGGGAGGCCGAGGTGCCGGACTTGGAG5130.10332971444339928No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC5090.10252402466216422No Hit
GCCCAGGGGTCCCTGACCGCTTCCTTGGCTCCAAGTCCGGCACCTCGGCCTCCCTGGCCATCAGTGACCTCCAAT5070.10212117977154665No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG5030.10131548999031158No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC4990.10050980020907649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)