FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496469
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34450.6939003240887145No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAATATGAGACCATTCAAAATGTCATCCCAAG30720.618769751988543No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTG27640.5567316388334418No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21230.4276198513905199No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCT20120.4052619599612463No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18460.3718258340399904No Hit
GATTATTATTGTGCAGCTTGGGATGACATTTTGAATGGTCTCATATTCGG17290.3482594079388643No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCC16980.34201531213429237No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16800.3383897081187345No Hit
ATAATAATCAGCCTCATCCTCAGATTGGAGGTCACTGATGGCCAGGGAGG16490.3321456123141626No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15980.32187306760341533No Hit
GTCTCATATTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC15580.3138161697910645No Hit
ATATGAGACCATTCAAAATGTCATCCCAAGCTGCACAATAATAATCAGCC15500.3122047902285943No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15110.30434931486155226No Hit
CATTTATAGTGATGATCAGTGGCGCCCAGGGGTCCCTGACCGCTTCCTTGGCTCCAAGTCCGGCACCTCGGCCTC14860.299313753728833No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13840.27876866430733843No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13710.2761501725183244No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAT13700.2759487500730156No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13530.2725245685027666No Hit
GTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC13420.2703089216043701No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12720.2562093504327561No Hit
CTCTTGCTCTGGGAGCAGCTCCAACATCGGAAGTCATTCTGTCAACTGGTACCAGTTAGTCCCAGGAACGGCCCC12090.2435197363783036No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11740.2364699507924966No Hit
CTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTAACTGGTACCAGTTGACAGAATGACTTCCGATGTTGGAG11510.23183723455039487No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11430.23022585498792472No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11350.22861447542545454No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11220.22599598363644055No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10790.2173348184881634No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10740.21632770626161957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10670.21491774914445816No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10580.2131049471366792No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10470.21088930023828276No Hit
CCTCCAATCTGAGGATGAGGCTGATTATTATTGTGCAGCTTGGGATGACA10140.2042423595430933No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9990.20122102286346177No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT9650.19437265972296358No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9190.18510722723876014No Hit
ACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAATATGAGACCATTCAAAA9150.18430153745752506No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9150.18430153745752506No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG9060.1824887354497461No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8880.17886313143418822No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8810.17745317431702684No Hit
GTACCAGTTAGTCCCAGGAACGGCCCCCAAACTCCTCATTTATAGTGATGATCAGTGGCGCCCAGGGGTCCCTGA8580.17282045807492513No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8550.1722161907389988No Hit
GGCCATCAGTGACCTCCAATCTGAGGATGAGGCTGATTATTATTGTGCAGCTTGGGATGACATTTTGAATGGTCT8400.16919485405936724No Hit
GACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAATATGAGACCATTCAAA8220.16556925004380937No Hit
GTCCGGCACCTCGGCCTCCCTGGCCATCAGTGACCTCCAATCTGAGGATG8170.16456213781726553No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA7990.16093653380170767No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7950.1601308440204726No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7860.15831804201269364No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC7850.15811661956738488No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7730.15569955022367962No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC7630.15368532577059194No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7580.15267821354404806No Hit
GGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGC7580.15267821354404806No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7500.15106683398157789No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7490.15086541153626912No Hit
AGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGG7480.15066398909096035No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT7480.15066398909096035No Hit
GATTGGAGGTCACTGATGGCCAGGGAGGCCGAGGTGCCGGACTTGGAGCCAAGGAAGCGGTCAGGGACCCCTGGG7400.1490526095284902No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7250.14603127284885864No Hit
GTTTGGGGGCCGTTCCTGGGACTAACTGGTACCAGTTGACAGAATGACTT7190.144822738177006No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7170.14441989328638846No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAATATGAGAC7140.14381562595046216No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC6890.1387800648177429No Hit
AACTGGTACCAGTTGACAGAATGACTTCCGATGTTGGAGCTGCTCCCAGA6870.13837721992712534No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6760.1361615730287289No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6750.13596015058342012No Hit
CCCCTGGGCGCCACTGATCATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTAACTGGTACCAGTTGA6680.1345501934662587No Hit
CTGATGGCCAGGGAGGCCGAGGTGCCGGACTTGGAGCCAAGGAAGCGGTC6570.13233454656786225No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA6540.13173027923193592No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC6460.13011889966946577No Hit
GTCCCTGACCGCTTCCTTGGCTCCAAGTCCGGCACCTCGGCCTCCCTGGC6460.13011889966946577No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6390.1287089425523044No Hit
GTGATGATCAGTGGCGCCCAGGGGTCCCTGACCGCTTCCTTGGCTCCAAG6170.12427764875551142No Hit
GATCAGTGGCGCCCAGGGGTCCCTGACCGCTTCCTTGGCTCCAAGTCCGG5970.120249199849336No Hit
GATCATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTAACTGG5840.117630708060322No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5830.11742928561501323No Hit
GTCAGGGACCCCTGGGCGCCACTGATCATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTAACTGGTA5820.11722786316970446No Hit
GGCAGAGGGTCACCATCTCTTGCTCTGGGAGCAGCTCCAACATCGGAAGT5770.1162207509431606No Hit
GTGCCGGACTTGGAGCCAAGGAAGCGGTCAGGGACCCCTGGGCGCCACTG5740.1156164836072343No Hit
GTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGCTCTGGGAGCAG5740.1156164836072343No Hit
GTCCCAGGAACGGCCCCCAAACTCCTCATTTATAGTGATGATCAGTGGCG5730.11541506116192551No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5700.11481079382599921No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC5640.11360225915414658No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5630.11340083670883781No Hit
GGGTCACCATCTCTTGCTCTGGGAGCAGCTCCAACATCGGAAGTCATTCT5570.11219230203698517No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC5540.11158803470105888No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAG5500.1107823449198238No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG5500.1107823449198238No Hit
CCACTGATCATCACTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGACTA5490.11058092247451504No Hit
GCTTGGTCCCCCCGCCGAATATGAGACCATTCAAAATGTCATCCCAAGCTGCACAATAATAATCAGCCTCATCCT5480.11037950002920624No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC5450.10977523269327995No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC5410.10896954291204487No Hit
GGGGTCCCTGACCGCTTCCTTGGCTCCAAGTCCGGCACCTCGGCCTCCCT5390.10856669802142731No Hit
GCCCCCAAACTCCTCATTTATAGTGATGATCAGTGGCGCCCAGGGGTCCC5370.10816385313080978No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGG5360.10796243068550102No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5360.10796243068550102No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC5350.10776100824019225No Hit
GCCCAGGGGTCCCTGACCGCTTCCTTGGCTCCAAGTCCGGCACCTCGGCCTCCCTGGCCATCAGTGACCTCCAAT5320.10715674090426591No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCATCCTCAGCGTCTGGGG5280.10635105112303085No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG5260.10594820623241331No Hit
GAGCAAGAGATGGTGACCCTCTGCCCGGGGGCCCCAGACGCTGAGGATGG5230.10534393889648698No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA5220.10514251645117821No Hit
GCTTGGGATGACATTTTGAATGGTCTCATATTCGGCGGGGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGG5090.10252402466216422No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC5090.10252402466216422No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT5060.10191975732623788No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC5050.10171833488092911No Hit
GCTCCAACATCGGAAGTCATTCTGTCAACTGGTACCAGTTAGTCCCAGGA4970.10010695531845895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)