FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354070
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAAGTC52990.3913387047936961No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAATTGACTCAGCCACTCTCAGTGT47910.3538221805371953No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46570.34392608949315767No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA43810.3235430959994683No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39780.29378097144165366No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39220.28964529160235436No Hit
CCTATGAATTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG36050.2662343896548923No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34750.2566337043136618No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG34520.254935121522521No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC33090.2443743676471674No Hit
GTTTATATCCGTATAGATGACCATCACAGGGGCCTGGCCTGGCTTCTGCT30770.22724083688435603No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29100.21490764879215993No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28840.21298751172391384No Hit
GCCTTGGGCTGACCTACGACGGTGACCGTGGTCCCAGGGCCGAAGACATAATTCGCAGTCCTGCTGTCCCACAC28580.21106737465566772No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAATTGACT28470.21025500897294824No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTTCCTGTGAGGGAAACAACATTGGAGGTAAAAATGTG28290.2089256833103163No Hit
GTAATAGAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA27820.20545466630233297No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25430.18780417555960918No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25260.18654870132267903No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25160.1858101870656613No Hit
GACTATCACTGTCAGGTGTGGGACAGCAGGACTGCGAATTATGTCTTCGG24020.17739112453565917No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTACGACGGTGACCGTGGTC23720.17517558176460596No Hit
GGCCAGGCCCCTGTGATGGTCATCTATACGGATATAAACCGGCCCTCTGG23520.17369855325057051No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23520.17369855325057051No Hit
GTCCCACACCTGACAGTGATAGTCAGCCTCATCCCCGGCTTGGGCTCTGTTGATGGTCAGGGTGGCCGTGTTCCC22230.16417171933504177No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTATAAAAGGTGTCCAGTGTGAAGTGCAACTGGTGGAGT20780.15346326260828466No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20580.1519862340942492No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20440.15095231413442436No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20240.1494752856203889No Hit
CCCTGGGACAGACGGCCAGGATTTCCTGTGAGGGAAACAACATTGGAGGT19850.14659508001801974No Hit
GTGATAGTCAGCCTCATCCCCGGCTTGGGCTCTGTTGATGGTCAGGGTGG19580.14460109152407186No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19290.14245940017872044No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19100.14105622309038676No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18810.13891453174503535No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18530.1368466918253857No Hit
CGGCCACCCTGACCATCAACAGAGCCCAAGCCGGGGATGAGGCTGACTATCACTGTCAGGTGTGGGACAGCAGGA18280.13500040618284137No Hit
GACCTACGACGGTGACCGTGGTCCCAGGGCCGAAGACATAATTCGCAGTC17790.13138168632345448No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG17720.13086472634354207No Hit
GGGTGGCCGTGTTCCCCGACTTGGAGCCAGAGAATCGCTCAGGGATCCCA17720.13086472634354207No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAATTGACTCAGCCACTCTCAGTGT17430.12872303499819063No Hit
CTCCAGGCCCTTCCCCGGAGCTTGCCGGACCCAGTGCATGGCATATTCAT17250.12739370933555874No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG17250.12739370933555874No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16830.12429194945608425No Hit
GGATTTCCTGTGAGGGAAACAACATTGGAGGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC16520.12200255525932928No Hit
GACCATCAACAGAGCCCAAGCCGGGGATGAGGCTGACTATCACTGTCAGG16460.12155944670511863No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16450.12148559527941688No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAAT15970.11794072684573176No Hit
TCGTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15940.11771917256862643No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15900.11742376686581936No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15890.11734991544011758No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15880.1172760640144158No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15730.1161682926288892No Hit
GTGTGGGACAGCAGGACTGCGAATTATGTCTTCGGCCCTGGGACCACGGT15260.11269727562090587No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15180.11210646421529168No Hit
CTCCTGTGCAGCCTCTGGATTCAAGTTTGATGAATATGCCATGCACTGGGTCCGGCAAGCTCCGGGGAAGGGC15160.11195876136388813No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC14930.11026017857274735No Hit
CCCCTGTGATGGTCATCTATACGGATATAAACCGGCCCTCTGGGATCCCT14580.10767537867318527No Hit
GTCCTGCTGTCCCACACCTGACAGTGATAGTCAGCCTCATCCCCGGCTTG14530.1073061215446764No Hit
GTTGATGGTCAGGGTGGCCGTGTTCCCCGACTTGGAGCCAGAGAATCGCT14390.1062722015848516No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14380.10619835015914983No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTACCTCCAATGTTGTTTCCCTCACAGGAAATCCTGGC14200.10486902449651792No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14130.10435206451660549No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT14110.10420436166520194No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14110.10420436166520194No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14080.10398280738809662No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13910.10272733315116649No Hit
CATCTATACGGATATAAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCGGGGAACACGGCCAC13850.10228422459695585No Hit
CAGCAGGACTGCGAATTATGTCTTCGGCCCTGGGACCACGGTCACCGTCGTAGGTCAGCCCAAGGCCAACCCCA13840.10221037317125407No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13640.10073334465721862No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13590.10036408752870975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)