FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354070
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATAAACCGGCCCTCTGGGATCCCTGAGCGATTCTCTGGCTCCAAGTC47180.34843102646096585No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC46180.34104588389078855No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAATTGACTCAGCCACTCTCAGTGT44050.3253155302163108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43440.32081059324850264No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42350.3127607878470094No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40740.3008707083090239No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36010.2659389839520852No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35590.26283722407261073No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33170.24496517905278162No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG33060.2441528133700621No Hit
CCTATGAATTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG30850.22783164828997024No Hit
GTTTATATCCGTATAGATGACCATCACAGGGGCCTGGCCTGGCTTCTGCT30810.22753624258716312No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAATTGACT29470.21764015154312555No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29330.21660623158330072No Hit
GCCTTGGGCTGACCTACGACGGTGACCGTGGTCCCAGGGCCGAAGACATAATTCGCAGTCCTGCTGTCCCACAC29100.21490764879215993No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26990.1993249979690858No Hit
GTAATAGAAGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATACA26760.19762641517794502No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTTCCTGTGAGGGAAACAACATTGGAGGTAAAAATGTG26690.1971094551980326No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26190.19341688391294393No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25320.18699180987688965No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24550.18130525009785314No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22910.16919361628276233No Hit
GACTATCACTGTCAGGTGTGGGACAGCAGGACTGCGAATTATGTCTTCGG22660.167347330640218No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTACGACGGTGACCGTGGTC22450.16579645070048077No Hit
GGCCAGGCCCCTGTGATGGTCATCTATACGGATATAAACCGGCCCTCTGG22120.16335935365232226No Hit
GGGTGGCCGTGTTCCCCGACTTGGAGCCAGAGAATCGCTCAGGGATCCCA21140.1561219139335485No Hit
GTCCCACACCTGACAGTGATAGTCAGCCTCATCCCCGGCTTGGGCTCTGTTGATGGTCAGGGTGGCCGTGTTCCC20850.15398022258819707No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20820.15375866831109175No Hit
GAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTATAAAAGGTGTCCAGTGTGAAGTGCAACTGGTGGAGT19900.14696433714652862No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19020.14046541168477256No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18970.1400961545562637No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18640.1376590575081052No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG18150.1340403376487183No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18130.13389263479731475No Hit
CCCTGGGACAGACGGCCAGGATTTCCTGTGAGGGAAACAACATTGGAGGT18120.133818783371613No Hit
GTGATAGTCAGCCTCATCCCCGGCTTGGGCTCTGTTGATGGTCAGGGTGG18110.13374493194591122No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAATTGACTCAGCCACTCTCAGTGT17400.12850148072108533No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16850.1244396523074878No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG16620.12274106951634702No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16460.12155944670511863No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16460.12155944670511863No Hit
CGGCCACCCTGACCATCAACAGAGCCCAAGCCGGGGATGAGGCTGACTATCACTGTCAGGTGTGGGACAGCAGGA16390.12104248672520623No Hit
CTCCTGTGCAGCCTCTGGATTCAAGTTTGATGAATATGCCATGCACTGGGTCCGGCAAGCTCCGGGGAAGGGC16160.11934390393406545No Hit
GGATTTCCTGTGAGGGAAACAACATTGGAGGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC16100.11890079537985482No Hit
GACCTACGACGGTGACCGTGGTCCCAGGGCCGAAGACATAATTCGCAGTC16070.1186792411027495No Hit
CTCCAGGCCCTTCCCCGGAGCTTGCCGGACCCAGTGCATGGCATATTCAT16040.11845768682564417No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15700.11594673835178387No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTACCTCCAATGTTGTTTCCCTCACAGGAAATCCTGGC15560.11491281839195906No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15510.1145435612634502No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15510.1145435612634502No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15460.11417430413494133No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAAT15410.11380504700643246No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15170.1120326127895899No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAATTGACTCAGC15050.11114639568116863No Hit
GTGTGGGACAGCAGGACTGCGAATTATGTCTTCGGCCCTGGGACCACGGT14830.1095216643157296No Hit
GTCCTGCTGTCCCACACCTGACAGTGATAGTCAGCCTCATCCCCGGCTTG14480.10693686441616757No Hit
GACCATCAACAGAGCCCAAGCCGGGGATGAGGCTGACTATCACTGTCAGG14380.10619835015914983No Hit
CCCCTGTGATGGTCATCTATACGGATATAAACCGGCCCTCTGGGATCCCT14320.10575524160493918No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14230.10509057877362324No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14090.1040566588137984No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGCTGGATTTTC14080.10398280738809662No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT14080.10398280738809662No Hit
GTTGATGGTCAGGGTGGCCGTGTTCCCCGACTTGGAGCCAGAGAATCGCT13950.10302273885397359No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13620.10058564180581507No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG306.791991E-445.32062
CTAACGC306.794464E-445.3172533
GGTATCA14700.041.409621
GTATCAA31550.034.168461
GTGGTAT4350.033.61591
TCTGGAG14300.031.85821770
TACGTAT850.00403592731.52751270
TGGTATC4650.030.7010542
GTCGTAC450.00496029230.228251
TAACGCC450.004972855630.2126184
CCCCCCT604.237436E-429.98688362
GTACGTA600.0057347429.34371269
TATCAAC36550.029.2011012
ATCAACG37150.028.8188463
TTAACGG605.625406E-428.57021535
TCAACGC37600.028.474994
CAACGCA38300.028.0412335
AACGCAG38550.027.9475466
ACGGTAC500.00832082627.19035310
CGCAGAG40300.026.6496098
Produced by FastQC (version 0.11.5)