FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194559
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGGGTATTGTCATGGGA42040.3519290382475876No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39690.33225650637599313No Hit
GGGTATTGTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGG36930.3091517455395673No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34250.2867166879157915No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32270.2701415334027034No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTC30390.2544035079054279No Hit
CCCAGGTACAGCCCCCAAACTCCTCATCCATGGTGACGCCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG29110.24368825650302747No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25340.2121284926068951No Hit
ACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCT23610.19764616063333834No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTACGCTGTGCTCGTGGATGTGTCTGTGGT22920.19186997042423187No Hit
GTACCTGGGAGGTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT22280.1865123447230317No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21820.18266155125029404No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21690.18157328352973776No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21360.1788107577775564No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21000.1757970933206313No Hit
GACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGG20820.17429026109216872No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGT20740.17362055787951872No Hit
GGTTATGATGTACACTGGTACCAGCACCTCCCAGGTACAGCCCCCAAACTCCTCATCCATGGTGACGCCAATCGG20390.17069060632417488No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19780.16558411932771844No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19710.16499812901664965No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTC19140.16022649362651822No Hit
GATGTACACTGGTACCAGCACCTCCCAGGTACAGCCCCCAAACTCCTCAT18980.1588870872012182No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18670.15629198725219937No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18500.15486886792531804No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18320.15336203569685547No Hit
GGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT18220.15252490668104296No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17780.14884153901146782No Hit
GTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG17330.14507445844031144No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17330.14507445844031144No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17300.1448233197355677No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17090.14306534880236138No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16840.14097252626283005No Hit
GGGCAGAGGGTCACCATCTCCTGCGCTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG16560.13862856501855497No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16210.1356986134632111No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16150.1351963360537236No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAG15860.13276866190786724No Hit
GGATGAGGCTGATTATTTCTGCCAGTCCCATGACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAG15550.1301735619588484No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15330.12833187812406086No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14800.12389509434025445No Hit
CTCCTGCGCTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTCCCAGGTACAGC14640.12255568791495437No Hit
GTGTATTACTGTGCGAGATCCCGTTATAGAGATGGTGGAGTGCTTATTATCGGTGATGTTTATGATGTCTGGGGC14430.12079771698174807No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTC14120.11820261703272923No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCCAT14100.11803519122956672No Hit
GTCCCATGACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGG13990.11711434931217293No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGC13970.11694692350901043No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13670.11443553646157285No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTC13400.112175288118879No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGCGCAGGAGA13370.11192414941413525No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13190.1104173171856727No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13160.11016617848092895No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCGCTGGGAGCAG13000.1088267720556289No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCC12930.10824078174456013No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12880.10782221723665386No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT12650.10589682050028504No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAG12650.10589682050028504No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCT12570.10522711728763502No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12530.10489226568131No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12530.10489226568131No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12490.104557414074985No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT12460.10430627537024123No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12370.10355285925600995No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG12190.1020460270275474No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA12190.1020460270275474No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12080.10112518511015361No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTCCCA11990.10037176899592236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGC200.00788594950.852757
AGGGGGG3150.043.12520270
CCTAACG451.11049754E-437.667132
GTGGTAT2750.036.982271
GTCGTAT905.2696123E-934.4469239
TCGCACG400.002825362133.9103522
CTCTTAT2000.033.9004171
GTATCAA34500.033.0160561
ATCAACG33550.032.5363163
GATGTCG959.404175E-932.4613336
TCAACGC33750.032.3435064
GGTATCA16400.032.0400281
TATACAC3600.032.017063
ATCTCCG3500.031.96458610
TCGTATG2750.031.37409640
CAACGCA34700.031.3616395
GACAGGT3150.031.2099061
ACGAGAC3600.031.08448822
AACGCAG35300.030.8285796
CTAACGC553.6096887E-430.818563
Produced by FastQC (version 0.11.5)