FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194559
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38450.3218761065799178No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37650.31517907445341753No Hit
GCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGGGTATTGTCATGGGA34900.292158026518573No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32660.2734063365643723No Hit
GGGTATTGTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGG32660.2734063365643723No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30520.25549177562598413No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG27450.2297919148405395No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTC25850.21639785058753902No Hit
CCCAGGTACAGCCCCCAAACTCCTCATCCATGGTGACGCCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG25810.216062998981214No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23560.1972275961254321No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22450.187935464049913No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21810.18257783834871277No Hit
ACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGGCTGCCCCCT21790.18241041254555027No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTACGCTGTGCTCGTGGATGTGTCTGTGGT21370.17889447067913766No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20940.1752948159111438No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20810.17420654819058748No Hit
GGTTATGATGTACACTGGTACCAGCACCTCCCAGGTACAGCCCCCAAACTCCTCATCCATGGTGACGCCAATCGG20140.16859778378464355No Hit
GGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT19750.16533298062297466No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGT18720.1567105517601056No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18440.15436659051583054No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18400.15403173890950553No Hit
GATGTACACTGGTACCAGCACCTCCCAGGTACAGCCCCCAAACTCCTCAT18090.15143663896048667No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17650.14775327129091154No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17480.14633015196403024No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17470.146246439062449No Hit
GTACCTGGGAGGTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT17000.14231193268813008No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16980.14214450688496758No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTC16630.13921455532962373No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC15980.1337732167268423No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15930.13335465221893603No Hit
GACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACTCAGG15870.1328523748094485No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15660.1310944038762422No Hit
GGATGAGGCTGATTATTTCTGCCAGTCCCATGACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAG15200.12724361040350457No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTC15190.1271598975019233No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC14990.12548563947029825No Hit
GGGCAGAGGGTCACCATCTCCTGCGCTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG14900.12473222335606697No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG14680.12289053952127942No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14610.12230454921021064No Hit
CTCCTGCGCTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACCTCCCAGGTACAGC14440.12088142988332932No Hit
TCGTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14310.11979316216277304No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14200.11887232024537925No Hit
GTCATGGGACTGGCAGAAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG14110.11811890413114799No Hit
GTGTATTACTGTGCGAGATCCCGTTATAGAGATGGTGGAGTGCTTATTATCGGTGATGTTTATGATGTCTGGGGC13960.11686321060742919No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13920.11652835900110418No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13720.11485410096947911No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13430.11242642682362278No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT13290.11125444620148524No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13230.11075216879199773No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13230.11075216879199773No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAG13080.10949647526827892No Hit
GTCCCATGACAATACCCTGAGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCGTAGGTCAGCCCAAGG12930.10824078174456013No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12800.10715251402400383No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCCAGTCCCAT12750.10673394951609756No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT12650.10589682050028504No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTACGACGGTCAGCTTGGTC12420.10397142376391622No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA12180.10196231412596617No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12130.10154374961805988No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAG12130.10154374961805988No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCGCTGGGAGCAG12120.10146003671647863No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCCTCCTCACTCT12010.10053919479908485No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11960.10012063029117858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)