FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671989
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATCTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT62970.9370689103541873No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC58770.8745678872719643No Hit
GAGTATTACTACGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCGT46740.6955470997293111No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30050.4471799389573341No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29430.4379535974547202No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20730.3084871924986867No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20330.30253471410990357No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17010.25312914348300347No Hit
GTATTACTACGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT16970.2525338956441251No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16900.25149221192608806No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16620.24732547705393987No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16500.24553973353730493No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15900.2366110159541302No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15570.2317002212833841No Hit
GTTGGAGACAGCGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTACGTGGTTGGCCTGGTATCAGCAGAAA15200.2261941787737597No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14550.21652140139198708No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGCGTCACCATCACTTGCCG14160.21071773496292348No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14160.21071773496292348No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14150.21056892300320396No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAT13640.2029795130575054No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACGCTGTCTCCAACAGA13560.20178901737974878No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13130.19539010311180688No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12990.19330673567573278No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12890.191818616078537No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12710.18914000080358456No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12500.18601494964947343No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12380.18422920613283847No Hit
GTCTGCATCTGTTGGAGACAGCGTCACCATCACTTGCCGGGCCAGTCAGA11740.17470524071078544No Hit
CAGTATAATAGTTATCTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11340.1687527623220023No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11080.16488365136929325No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT10760.16012166865826674No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA10670.15878236102079052No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC10460.15565730986667936No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10280.15297869459172694No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTTAATGGGACCCCA10000.14881195971957875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9880.1470262162029438No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9710.14449641288771098No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9650.1436035411293935No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9380.13958561821696486No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9230.13735343882117118No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC9060.13482363550593834No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8740.1300616527949118No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8730.12991284083519225No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC8660.1288711571171552No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8590.12782947339911813No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG8510.1266389777213615No Hit
TAGTAATACTCTGACTGGCCCGGCAAGTGATGGTGACGCTGTCTCCAACAGATGCAGACAGGGTGGAAGGAGA8420.1252996700838853No Hit
ACCTTAATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG8190.121876995010335No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA8170.12157937109089584No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8170.12157937109089584No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT8050.11979362757426087No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7960.11845431993678468No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7870.11711501229930846No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7760.11547808074239312No Hit
GTGTATTACTGTGCGAGACAGTTCAATTACGATTTTTGGAGTGGTTATTCCCCATATTACTTTGACTACTGGGGC7690.11443639702435604No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7680.11428758506463647No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTTGGAGACAGCGTCACCATCACTT7650.11384114918547773No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7620.11339471330631899No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7590.11294827742716025No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7580.11279946546744068No Hit
ACTTATTACTGCCAACAGTATAATAGTTATCTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA7440.11071609803136657No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7440.11071609803136657No Hit
CATTAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG7390.10997203823276869No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCG7300.10863273059529249No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7190.10699579903837711No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7140.10625173923977922No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7130.10610292728005964No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7120.10595411532034008No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC7120.10595411532034008No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCACGTAGTAATACTCTGA7040.10476361964258343No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7000.10416837180370511No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6950.10342431200510721No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6930.10312668808566806No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6910.10282906416622892No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6840.10178738044819187No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA6770.1007456967301548No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC6740.10029926085099608No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6740.10029926085099608No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6730.1001504488912765No Hit
ATCTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC6720.1000016369315569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATACG100.009795779130.1300570
TCGGAAC150.00237075268.8849140
GTCTCGT150.002455136368.280036
TCTATAC252.7028876E-454.6240233
GTACTAC200.00766698451.2138331
ATCTCGT603.005989E-740.0805637
TCGTATG350.001358512739.36280440
CATCTCG754.1931344E-836.620436
CGAGACT1051.8189894E-1135.78703723
CCCACGA1051.8189894E-1135.76572819
TTGCCGA400.00234816135.2117256
GTATCAA16600.034.9652631
GCCGTCT501.7975165E-434.7029946
TGTTAGA7750.034.42149770
ATCTCCG1103.092282E-1134.1425510
CTTATAC1103.092282E-1134.142551
GGCAGTA12400.032.7658414
ACGAGAC1155.2750693E-1132.66538622
ACGAACT14800.032.16906752
AACGAAC15300.031.976951
Produced by FastQC (version 0.11.5)