FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730766
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGAGGCTGTCCGGGTCTCTGCTGGTACCAGGCTAAGTGGTTCATATTCCTGGAGGTGGAGAAAAGAGT13670.1870639849144596No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11820.16174808351784292No Hit
CTCCTAGGCTGCTCATTTACTGGACTTCTACGCGGGTATCCGGGGTCCCT11380.1557270042667557No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9710.1328742716546747No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9100.12452686632930378No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8750.1197373714704844No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8530.11672683184494079No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8250.1128952359578853No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7970.10906364007082978No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7730.10577941502478222No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT200.008261980550.2549927
ACGCGTA652.8558134E-1046.3892227
GACGCGT652.8740033E-1046.34153426
GGTATCA8700.039.561721
GTATCAA20350.036.782461
ATGCCGT852.7812348E-936.77077544
AGGTACG559.1994025E-636.454052
CCCGTTT400.002228792835.58843251
ACGGTTA500.003418950632.61337368
CGTAGTA951.1392331E-831.82514630
CGAGACG951.19289325E-831.674823
AACGCAG24800.030.451876
GGTACGG553.9257342E-430.3783723
CGTATGC802.8336108E-630.08529341
TCAACGC25150.030.0280864
ATCAACG25200.029.9685083
CGATCCG450.005360830629.7501226
AGACGCG902.614761E-729.7317825
TATCAAC25250.029.6444822
CAACGCA25550.029.5579785
Produced by FastQC (version 0.11.5)