FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730766
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGAGGCTGTCCGGGTCTCTGCTGGTACCAGGCTAAGTGGTTCATATTCCTGGAGGTGGAGAAAAGAGT12520.17132707323548169No Hit
CTCCTAGGCTGCTCATTTACTGGACTTCTACGCGGGTATCCGGGGTCCCT10090.13807429464425No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9950.13615849670072228No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9590.1312321591316509No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8340.11412682035015313No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT8320.11385313492964916No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8320.11385313492964916No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8220.11248470782712933No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7990.10933732549133375No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7340.1004425493249549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)