FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204902
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55430.4600374138311663No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCCCCGCCGAATATCACATCATTGCCGCCTCCATATGAGGAG43320.359531314579941No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42100.3494060097833683No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38750.3216029187435991No Hit
GACCTAGGACGGTCACCCTGGTCCCCCCGCCGAATATCACATCATTGCCGCCTCCATATGAGGAGCAGAGGTAGT35380.29363383910060736No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33630.27910983631863834No Hit
GTCTTGGGCCCGTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCACCATCACCTG32400.26890153722045446No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCCC32210.26732464548984064No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG30810.2557054432642655No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30160.2503108136595341No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28270.23462489065500763No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27880.23138811289216882No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26920.2234206599374887No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25710.21337834944252726No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25150.2087306685522972No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25020.20765174263135092No Hit
GTGTGGGGGCTTTGTCTGGCAGTTGCTGGTACCAGGAGATGAAGTCAAAA24060.19968428967667082No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23940.19868835805733576No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23680.19653050621544324No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23600.19586655180255322No Hit
GGACACAGGGTCTTGGGCCCGTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCAC23550.19545158029449697No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23250.19296175124615944No Hit
GAGGTAGTCAGCCTCATCCTCAAATTGGACCCCCGAGACGGTCAGGGAGG22950.1904719221978219No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22810.18931000197526437No Hit
GACTACCTCTGCTCCTCATATGGAGGCGGCAATGATGTGATATTCGGCGGGGGGACCAGGGTGACCGTCCTAGGT22710.18848005895915187No Hit
GTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCA22110.1835004008624768No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21880.181591531925418No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21350.17719283394002167No Hit
CCTCATATGGAGGCGGCAATGATGTGATATTCGGCGGGGGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTG20920.17362407897073787No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC20890.17337509606590412No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20660.17146622712884532No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20040.16632058042894776No Hit
GTCAGGGAGGCCGTGTCTCCAGACCTGGAGCCAGAGAAGCGATCAGGGAC19830.16457770009511147No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19760.1639967399838327No Hit
CTCCAGACCTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGTCGCTTA18730.15544832691787383No Hit
CCCTGGTCCCCCCGCCGAATATCACATCATTGCCGCCTCCATATGAGGAG18040.1497217201066975No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGACACAGGGTCTTGGGCCCGTTCTGCCCTGACTCAGC17950.14897477139219623No Hit
CCCCTGAGGGTCGCTTATTGACTTCATAAATGAGGAGTGTGGGGGCTTTG17840.14806183407447246No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17300.14358014178746487No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17120.14208624435846234No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16850.13984539821495856No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCCCCGCCGAATATCACAT16620.1379365292778998No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16580.13760455207145478No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16490.13685760335695352No Hit
TCCTCACCCTCCTCACTCAGGACACAGGGTCTTGGGCCCGTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGT16140.1339528028005597No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15840.13146297375222218No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15700.13030105352966465No Hit
GACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCACCATCA15600.12947111051355215No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGACACAGGGTCTTGGGCCCGTTCTGCCCTGACT15540.12897314470388463No Hit
GAGCAGAGGTAGTCAGCCTCATCCTCAAATTGGACCCCCGAGACGGTCAGGGAGGCCGTGTCTCCAGACCTGGAG15430.12806020738616086No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15390.12772823017971588No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCCCCGCCGAATATCACATCATTGC15180.12598534984587959No Hit
GCCCCCACACTCCTCATTTATGAAGTCAATAAGCGACCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAGGT15100.12532139543298956No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15010.1245744467184883No Hit
CTCCCTGACCGTCTCGGGGGTCCAATTTGAGGATGAGGCTGACTACCTCT14840.12316354359109703No Hit
GCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCACCATCACCTGCA14770.12258258347981826No Hit
GGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14590.12108868605081576No Hit
CCTCTGCTCCTCATATGGAGGCGGCAATGATGTGATATTCGGCGGGGGGACCAGGGTGACCGTCCTAGGTCAGCC14550.12075670884437076No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14270.11843286839925571No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAGGTCTGGAGACACGGCC14180.11768591968475445No Hit
CCATCACCTGCACCTCAAGCAGCGGTGACTTTCATGGTTTTGACTTCATC14170.11760292538314318No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCAC14060.11668998806541941No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13940.11569405644608441No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13780.11436614762030438No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13610.11295524449291311No Hit
ACCTAGGACGGTCACCCTGGTCCCCCCGCCGAATATCACATCATTGCCGC13600.11287225019130187No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13470.1117933242703556No Hit
CTTATTGACTTCATAAATGAGGAGTGTGGGGGCTTTGTCTGGCAGTTGCT13320.11054840974618683No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13000.10789259209462679No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12990.10780959779301554No Hit
GGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAG12890.10697965477690302No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12810.106315700364013No Hit
GTCAATAAGCGACCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAGGTCTGGAGACACGGCCTCCCTGACCGT12800.10623270606240176No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC12570.10432383712534299No Hit
GGTCTGGAGACACGGCCTCCCTGACCGTCTCGGGGGTCCAATTTGAGGAT12510.10382587131567546No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12380.10274694539472919No Hit
GTCAGTCACCATCACCTGCACCTCAAGCAGCGGTGACTTTCATGGTTTTGACTTCATCTCCTGGTACCAGCAA12350.10249796248989546No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12230.10150203087056042No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12220.10141903656894918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1750.051.36444541
TCGTATG1950.049.6026540
GGTATCA11850.046.2166561
TATACAC2750.043.5536043
ATCTCCG2700.043.08735310
ATCTCGT1600.043.0402337
TCTCCGA2800.042.7705411
ACGAGAC2650.042.6162122
TCTCGTA1550.042.24247438
CTTATAC2750.041.0750541
TTATACA2750.041.0648272
TACACAT2950.040.6008155
ATGCCGT2150.040.35083444
GCCGTCT2250.040.1877146
TAAGACA2950.039.54089731
TACTAGA350.001402789139.109362
CACGAGA2900.038.9391721
CCCACGA3000.038.78183719
CCCTAAG3150.038.06564328
AATGGTA5700.036.8935970
Produced by FastQC (version 0.11.5)