FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204902
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51010.4233539325189933No Hit
GTCTTGGGCCCGTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCACCATCACCTG40470.33587793862073434No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39620.3288234229837779No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37220.3089047905970776No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35200.29213994167160484No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31630.2625109759963881No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30550.25354759142237293No Hit
GGACACAGGGTCTTGGGCCCGTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCAC29540.24516516695963653No Hit
GTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCA29450.24441821824513527No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27840.23105613568572383No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC27790.23064116417766756No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27450.22781935792288502No Hit
GTGTGGGGGCTTTGTCTGGCAGTTGCTGGTACCAGGAGATGAAGTCAAAA26520.2201008878730386No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26380.21893896765048113No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25730.2135443380457498No Hit
GCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCCCCGCCGAATATCACATCATTGCCGCCTCCATATGAGGAG25020.20765174263135092No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23740.19702847202511076No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23670.196447511913832No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22680.1882310760543181No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22520.1869031672285381No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22110.1835004008624768No Hit
GACTACCTCTGCTCCTCATATGGAGGCGGCAATGATGTGATATTCGGCGGGGGGACCAGGGTGACCGTCCTAGGT21790.18084458321091676No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21540.17876972567063545No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21410.17769079974968918No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGACACAGGGTCTTGGGCCCGTTCTGCCCTGACTCAGC21350.17719283394002167No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21280.1766118738287429No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21160.1756159422094079No Hit
GCCCCCACACTCCTCATTTATGAAGTCAATAAGCGACCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAGGT20360.1689763980805078No Hit
CCTCATATGGAGGCGGCAATGATGTGATATTCGGCGGGGGGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTG20270.16822944936600653No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCCTGGTCCCCC20200.16764848925472775No Hit
GACCTAGGACGGTCACCCTGGTCCCCCCGCCGAATATCACATCATTGCCGCCTCCATATGAGGAGCAGAGGTAGT19830.16457770009511147No Hit
CTCCAGACCTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGTCGCTTA19120.15868510468071262No Hit
GACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCACCATCA19070.15827013317265637No Hit
GCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCACCATCACCTGCA18950.15727420155332134No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18940.1571912072517101No Hit
GTCAGGGAGGCCGTGTCTCCAGACCTGGAGCCAGAGAAGCGATCAGGGAC18680.15503335540981755No Hit
TCCTCACCCTCCTCACTCAGGACACAGGGTCTTGGGCCCGTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGT18620.15453538960015006No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAGGTCTGGAGACACGGCC18370.15246053205986876No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18150.15063465742442125No Hit
GTCAATAAGCGACCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAGGTCTGGAGACACGGCCTCCCTGACCGT18010.14947273720186371No Hit
CTCCTCATTTATGAAGTCAATAAGCGACCCTCAGGGGTCCCTGATCGCTT17840.14806183407447246No Hit
CCCCTGAGGGTCGCTTATTGACTTCATAAATGAGGAGTGTGGGGGCTTTG17740.14723189105835993No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17550.14565499932774614No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17410.14449307910518863No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGACACAGGGTCTTGGGCCCGTTCTGCCCTGACT17390.14432709050196613No Hit
CCATCACCTGCACCTCAAGCAGCGGTGACTTTCATGGTTTTGACTTCATC17160.14241822156490735No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16980.14092432413590483No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16940.14059234692945982No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16820.1395964153101248No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCAC16810.13951342100851355No Hit
GTCAGTCACCATCACCTGCACCTCAAGCAGCGGTGACTTTCATGGTTTTGACTTCATCTCCTGGTACCAGCAA16360.13577867743600724No Hit
CTCCCTGACCGTCTCGGGGGTCCAATTTGAGGATGAGGCTGACTACCTCT16110.13370381989572597No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16000.1327908825780022No Hit
GGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAG15880.13179495095866717No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC15750.13071602503772092No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15750.13071602503772092No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15240.1264833156555471No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15140.12565337263943457No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14850.1232465378927083No Hit
GGTCTGGAGACACGGCCTCCCTGACCGTCTCGGGGGTCCAATTTGAGGAT14370.11926281141536822No Hit
CCTCTGCTCCTCATATGGAGGCGGCAATGATGTGATATTCGGCGGGGGGACCAGGGTGACCGTCCTAGGTCAGCC13730.11395117611224814No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13650.11328722169935812No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13580.11270626158807937No Hit
GTTTTGACTTCATCTCCTGGTACCAGCAACTGCCAGACAAAGCCCCCACA13500.11204230717518934No Hit
CTTTCATGGTTTTGACTTCATCTCCTGGTACCAGCAACTGCCAGACAAAGCCCCCACACTCCTCATTTATGAAGT13470.1117933242703556No Hit
GGGTCTTGGGCCCGTTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGCGTCACCTGGGCAGTCAGTCACCATCAC13430.1114613470639106No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13410.1112953584606881No Hit
CACCTGCACCTCAAGCAGCGGTGACTTTCATGGTTTTGACTTCATCTCCTGGTACCAGCAACTGCCAGACAAAGC13350.11079739265102058No Hit
GGACCAGGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG12990.10780959779301554No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12670.10515378014145549No Hit
ACCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAGGTCTGGAGACACGG12660.10507078583984422No Hit
CTTATTGACTTCATAAATGAGGAGTGTGGGGGCTTTGTCTGGCAGTTGCT12270.10183400807700542No Hit
CCCTGACCGTCTCGGGGGTCCAATTTGAGGATGAGGCTGACTACCTCTGC12270.10183400807700542No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12200.10125304796572668No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12170.10100406506089292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG100.00854592136.2189570
GGTATCA12550.044.6811261
GTGGTAT3600.042.739951
TGGTATC3950.037.199992
TACGTAT750.002015413736.32505470
GTATCAA29100.033.6044651
TAACGGC851.2381315E-732.3810936
TCGAAGG500.00467518530.59578168
CAACGCT450.004832046630.39032723
TATCAAC32150.029.9736482
ATGCATG1852.8384602E-729.45274470
TCAACGC32600.029.341734
ATCAACG32700.029.2544293
CGTGCAA853.7475984E-629.02747257
CAACGCA33300.028.7249365
TTAACGG854.5480865E-628.31451835
AACGCAG33800.028.198946
ATGTAGA500.00808106427.3535671
ACGCTTG500.00808106427.35356725
GATTGAC1405.2023097E-1026.8651121
Produced by FastQC (version 0.11.5)