FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212495
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGGTCCAACAATAAGAACTACTTAGCTTGGTACCAGCACAA29220.24099068449766806No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT25080.2068462137988198No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25030.20643384096429265No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23570.19439255419609977No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT23540.19414513049538348No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGGAGATACA23500.19381523222776176No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22730.1874646905760436No Hit
ATATTATAGTATTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT22480.18540282640340786No Hit
GTATATTACTGTGCGAAAGACCAAGACAACGGTGGTGCCTTTGACTACTGGGGCCTGGGAACCCCGGTCACCGT19660.16214499853607645No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19130.15777384649008863No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18840.15538208404983114No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA18310.15101093200384333No Hit
GTATAAAACACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18190.15002123720097815No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT17760.14647483082404464No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17340.14301089901401656No Hit
CTATAATATTGCTGACAGAAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC17310.14276347531330028No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16560.13657788279539299No Hit
GTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGAGCACGCTGTATCTCCAAATGAG16190.13352632381989205No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16190.13352632381989205No Hit
GCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGACCTGTATAAAACACT16090.13270157815083772No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15610.12874279893937707No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC15570.12841290067175534No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15530.12808300240413364No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15440.12734073130198476No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14680.12107266421717204No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14400.11876337634381998No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG14300.11793863067476568No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA14150.11670151217118421No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14090.11620666476975162No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13530.11158808902304751No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13520.11150561445614209No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13290.10960869941731717No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13100.10804168264611401No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13090.10795920807920859No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13050.10762930981158686No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12580.1037530051670316No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG12250.1010313444591524No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC12130.10004164965628724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGA150.002233605669.9311946
TACGTAT553.5659593E-451.6413570
CGTATGC1250.044.219341
TCGTATG1300.042.40855840
GGTATCA14450.040.8137931
TCTCCGA1600.039.7887211
ATGCCGT1500.037.1097744
GTATCAA33650.034.6542361
ATCTCCG1800.033.50628710
GTACGTA852.2398122E-532.01236769
GTGGTAT4750.030.3345091
CGAGACT2000.030.18802823
ACGAGAC2000.030.17930622
CCCACGA2000.030.16685519
TGGTATC4950.029.7870542
TATCAAC39300.029.6733632
ATCAACG40500.028.9596373
TCAACGC40550.028.8412884
GGTACGT705.380442E-428.78758468
CCGTCTT1950.028.7639947
Produced by FastQC (version 0.11.5)