FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212495
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGGTCCAACAATAAGAACTACTTAGCTTGGTACCAGCACAA24770.20428950222475145No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23760.19595957096730296No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21310.17575330207547246No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21180.17468113270570187No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGGAGATACA20350.16783574365255113No Hit
ATATTATAGTATTCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT20160.16626872688134797No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19810.16338211703965788No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18840.15538208404983114No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18680.15406249097934424No Hit
CTATAATATTGCTGACAGAAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC18580.15323774531028994No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT18330.15117588113765418No Hit
GTATATTACTGTGCGAAAGACCAAGACAACGGTGGTGCCTTTGACTACTGGGGCCTGGGAACCCCGGTCACCGT17710.14606245798951747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15930.13138198508035084No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15760.12997991744295853No Hit
GTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAGGAGCACGCTGTATCTCCAAATGAG15540.12816547697103906No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT14700.12123761335098289No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14510.11967059657977971No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA14490.11950564744596884No Hit
GCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGACCTGTATAAAACACT14470.11934069831215799No Hit
GTATAAAACACTCTGGCTGGACTTGCAGCTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA14460.11925822374525255No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14160.11678398673808964No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14150.11670151217118421No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13650.1125777838259127No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13500.11134066532233122No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13120.10820663177992487No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13000.1072169369770597No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12280.1012787681598687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACG200.00621917853.99967256
CCGATAC252.935011E-453.72377425
TACGTAT605.057084E-448.11063470
GGTATCA14650.038.4303931
GTGGTAT4150.037.9535981
GTATCAA35200.035.7971421
GGTACGT602.1208245E-433.7417568
GAACGGT450.002935987233.6453766
GTTTAGC400.002993755733.512221
CCTAACG601.6412572E-533.502542
GCTCGAC400.003006496233.48319611
AGCGACG450.003265757332.92242463
AAACGAC450.003809344731.90360555
TGGTATC5050.031.8439962
AGATCGT450.004496898530.8401543
TATCAAC40700.030.70382
CGTGCTC7500.029.9161452
TCAACGC41900.029.7297744
TGGTACG654.5446277E-429.63007467
ATCAACG42150.029.5595383
Produced by FastQC (version 0.11.5)