FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862943
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42660.494354783572032No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31750.36792696620750154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29930.3468363495619062No Hit
AGCCAGGACAGTCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA28880.33466868611252426No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT28730.33293044847689823No Hit
GTTTATTACTGTCAGCAATATTTTAGTCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26670.3090586516143013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25970.30094687598138No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25250.29260333533037525No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24700.28622979733307996No Hit
ACTTAGGAGACTGTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTTCTGATTGTTGGAGCTGTAAAAAACACT24290.2814786144623689No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24120.27950861180865944No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23360.27070154112148775No Hit
GTAATAAACTGCCACATCTTCAGTCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA23360.27070154112148775No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20740.24034032375255376No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20320.235473258372801No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19810.22956325041167258No Hit
GTCCTGGCTTCTGCTGATACCAAGCTAAGTAGTTCTGATTGTTGGAGCTG18520.2146144067452891No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18340.2125285215825379No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18290.21194910903732925No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18120.20997910638361977No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17820.20650263111236777No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17660.2046485109677001No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17150.19873850300657167No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17080.19792732544327957No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16850.19526202773531973No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16570.1920173174821512No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGACTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTCCT16320.1891202547561079No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16090.18645495704814802No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15890.18413730686731336No Hit
GATATAAACTATGCGGACTCCGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACACATTGTATCTG15240.17660494377960073No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14960.17336023352643223No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAA14860.1722014084360149No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTA14750.1709267008365558No Hit
ATTTTAGTCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA14710.17046317080038892No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT14120.16362610276692666No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC13930.16142433509513374No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13270.1537760894983794No Hit
GTTTTTTACAGCTCCAACAATCAGAACTACTTAGCTTGGTATCAGCAGAA13260.15366020698933763No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13240.1534284419712542No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13190.15284902942604553No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12970.1502996142271274No Hit
GAACTACTTAGCTTGGTATCAGCAGAAGCCAGGACAGTCTCCTAAGTTGC12880.1492566716457518No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG12820.14856137659150143No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12570.14566431386545808No Hit
CAGTAATAAACTGCCACATCTTCAGTCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA12530.14520078382929116No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC12380.14346254619366516No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11910.13801606826870372No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11810.1368572431782864No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11720.1358143005969108No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11650.13500312303361867No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11510.13338076790703443No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGACTGTCCTGGCTTCTGCTGATA11480.13303312037990922No Hit
GTCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT11390.13199017779853361No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11140.1290931150724903No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11030.12781840747303125No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGACTGAAGATG11010.12758664245494777No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10950.12689134740069738No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10840.12561663980123833No Hit
TTATTACTGTCAGCAATATTTTAGTCCTCCGCTCACTTTCGGCGGAGGGA10790.12503722725602967No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTACAGCTCCAACAATCAGAACTA10780.12492134474698792No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10770.1248054622379462No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10750.12457369721986272No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10720.12422604969273754No Hit
CACCATCAGCAGCCTGCAGACTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTTTAGTCCTCCGCTCACTTT10580.12260369456615326No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10390.12040192689436034No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10330.11970663184010995No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10190.11808427671352568No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10150.11762074667735875No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10130.11738898165927529No Hit
GTTTATATCACTGCCATCACTATTAATACGTGAGACCCACACCAGCCCCTTCCCTGGAGCTTGGCGGACCCAGTG10110.11715721664119184No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT10070.1166936866050249No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9890.11460780144227371No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTTCTCGATGCACTGGGTCCGCCAAGCTCCAGGGAAGGGG9880.11449191893323196No Hit
ATATTTTAGTCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9840.11402838889706506No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9690.11229015126143906No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT9500.11008838358964612No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9430.10927720602635399No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGGTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG9310.1078866159178532No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9230.10695955584551936No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGACTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGT9170.10626426079126895No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9160.1061483782822272No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9120.1056848482460603No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9110.10556896573701854No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9060.10498955319180989No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8960.10383072810139257No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8810.10209249046576657No Hit
GGACTAAAATATTGCTGACAGTAATAAACTGCCACATCTTCAGTCTGCAGGCTGCTGATGGTGAGAGTGAAAT8790.1018607254476831No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8740.10128131290247444No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8720.10104954788439098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAT200.00665501353.08158559
GACTGCG200.007663754351.220537
GCTTATA352.4082612E-548.804081
GTATCAA21900.035.566811
AGTCTTG400.002710122634.19854724
TATCAAC23950.032.3723222
AAGACGC450.00437907931.00697349
AACGCAG25400.030.7860956
GTGGTAT2800.030.5025521
CAACGCA25650.030.4860345
CGCAGAG26750.029.1047528
ACGCAGA26950.029.0154677
AGAGTAC27050.028.908211
GGCATAC605.4220704E-428.74907339
ACGGCAG605.574286E-428.61337733
CACGGCA605.5971334E-428.59338432
CAGAGTA27400.028.53893310
TGTTAGA10600.028.28632470
GCAGAGT27750.027.9328789
ACGGGTA756.582687E-527.33345816
Produced by FastQC (version 0.11.5)