FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557693
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25340.45437184974528994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22280.3995029523411626No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19010.34086854237008535No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16650.2985513535224577No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15750.2824134425212438No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC15740.2822341323990081No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGTACCTAGAGCCTGAAGATT14420.2585651962638943No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13850.24834451929645882No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13270.23794453220678757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13160.23597212086219477No Hit
GTGTTAGCACCAACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCC12930.23184798805077345No Hit
GTTTATTACTGTCAGCAACGTAGCGACTGGCCTACGTTCGGCCAAGGGAC12680.2273652349948807No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGTCTGGTA12530.22467558316134503No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12500.2241376527946379No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12150.21786179851638804No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12020.21553076692732379No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT11980.21481352643838098No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11960.21445490619390958No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11570.20746181142671685No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCAGCTTGGTCC11400.2044135393487098No Hit
GTCAGAGTGTTAGCACCAACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATG11170.20028940653728844No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11120.19939285592610986No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11000.19724113445928138No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT10890.19526872311468854No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10840.19437217250351No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT10780.19329631177009574No Hit
GACTTCACTCTCACCATCAGGTACCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAACGTAGCGACTGG10750.1927583814033886No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10500.18827562834749587No Hit
GGCCTACGTTCGGCCAAGGGACCAAGCTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC9890.17733771089111752No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9160.16424807196791066No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA9130.16371014160120353No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8870.15904807842307506No Hit
AACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCC8610.1543860152449466No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8460.15169636341141093No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8380.15026188243352526No Hit
ACGTAGGCCAGTCGCTACGTTGCTGACAGTAATAAACTGCAAAATCTTCA8380.15026188243352526No Hit
GGTTTGTGCTGGTACCAGACTAAGTTGGTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC8210.14721361035551817No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT8180.14667567998881104No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8110.14542050913316107No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8060.14452395852198252No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC7860.1409377560772683No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7810.14004120546608978No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7710.13824810424373268No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7580.13591707265466843No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7570.13573776253243272No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7510.13466190179901846No Hit
GTGCAGCCACAGTTCGCTTGATTTCCAGCTTGGTCCCTTGGCCGAACGTA7460.13376535118783991No Hit
GCCAAGGGACCAAGCTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTC7440.13340673094336847No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC7350.13179293984324708No Hit
GGTACCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAACGTAGCGACTGGCCTACGTTCGGCCAAGGGA7210.12928259813194715No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7170.1285653576430043No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7160.1283860475207686No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGTCTGGTACCAGCACAAACCTGGCCAGG7060.12659294629841147No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC6990.1253377754427615No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6870.12318605397593299No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6760.12121364263134018No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGGTA6640.11906192116451166No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6610.11852399079780453No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6550.11744813006439025No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6530.11708950981991885No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6500.11655157945321172No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6500.11655157945321172No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTC6420.11511709847532603No Hit
TTATTACTGTCAGCAACGTAGCGACTGGCCTACGTTCGGCCAAGGGACCAAGCTGGAAATCAAGCGAACTGTGG6390.11457916810861889No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6340.11368261749744035No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6280.1126067567640261No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6270.11242744664179039No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6270.11242744664179039No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT6240.11188951627508326No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6210.11135158590837611No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6190.1109929656639047No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6170.11063434541943326No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6160.11045503529719755No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6130.10991710493049042No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6120.10973779480825473No Hit
CTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCC6080.10902055431931189No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGGTACC6030.10812400370813333No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA5990.10740676321919049No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG5930.10633090248577623No Hit
GCCACAGTTCGCTTGATTTCCAGCTTGGTCCCTTGGCCGAACGTAGGCCA5910.1059722822413048No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT5850.10489642150789055No Hit
GTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCCACTGGCATC5850.10489642150789055No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC5820.10435849114118342No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5690.10202745955211918No Hit
AACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCCA5620.1007722886964692No Hit
CTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCCACTGGCATCCCA5600.10041366845199778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAT350.00525711456.33206670
CCGTGAG200.007658626451.22681413
CGTATGC1300.047.8224241
GGTATCA4800.046.254731
TCGTATG1350.045.9803240
ATGCGCA1400.043.9757529
GCCGTCT1350.043.71087646
GACATGC1450.042.42123826
TATACAC1550.041.859023
CTTATAC1400.041.476771
GCGCAGA1500.041.09940731
ATCTCGT1350.040.7569737
TGCCGTC1450.040.64446345
ATGCCGT1450.040.6148844
GTATGCC1450.040.51168442
AATAGCG458.104949E-539.7310162
CATGCGC1550.039.70576528
ACATGCG1550.039.68794627
CACGAGA1550.039.65591421
GATCTCG1400.039.2588236
Produced by FastQC (version 0.11.5)