FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557693
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25550.4581373623122399No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18220.32670304271346423No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17780.31881339733509295No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17130.3071582393897718No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14030.25157210149670156No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAC13930.2497790002743445No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13830.24798589905198737No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGGTACCTAGAGCCTGAAGATT13820.2478065889297517No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGTCTGGTA12890.23113074756183064No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12840.23023419695065206No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12790.2293376463394735No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12450.22324110218345938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12410.22252386169451652No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCT12390.2221652414500451No Hit
GTGTTAGCACCAACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCC12380.2219859313278094No Hit
GTTTATTACTGTCAGCAACGTAGCGACTGGCCTACGTTCGGCCAAGGGAC12190.2185790390053309No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11560.20728250130448111No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11410.20459284947094547No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11040.19795837494822419No Hit
GACTTCACTCTCACCATCAGGTACCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAACGTAGCGACTGG10920.19580665348139567No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCAGCTTGGTCC10700.19186183079221006No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10530.188813558714203No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10510.18845493846973155No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10230.18343425504713165No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10160.18217908419148168No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT10140.18182046394701026No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT9950.17841357162453178No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9920.17787564125782462No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9680.1735721983241676No Hit
GTCAGAGTGTTAGCACCAACTTAGTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATG9490.1701653060016891No Hit
GGCCTACGTTCGGCCAAGGGACCAAGCTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC9270.16622048331250347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9130.16371014160120353No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC9090.1629929011122607No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8630.154744635489418No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA8610.1543860152449466No Hit
GGTTTGTGCTGGTACCAGACTAAGTTGGTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC8210.14721361035551817No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8200.14703430023328248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8080.14488257876645394No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT7840.1405791358327969No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7650.1371722435103184No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7640.1369929333880827No Hit
GTGCAGCCACAGTTCGCTTGATTTCCAGCTTGGTCCCTTGGCCGAACGTA7490.13430328155454704No Hit
ACGTAGGCCAGTCGCTACGTTGCTGACAGTAATAAACTGCAAAATCTTCA7420.13304811069889705No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC7390.13251018033218995No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7170.1285653576430043No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7070.1267722564206472No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6850.12282743373146159No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6840.12264812360922586No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6800.12193088312028302No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6770.1213929527535759No Hit
AACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCC6770.1213929527535759No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6770.1213929527535759No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6700.12013778189792591No Hit
TTATTACTGTCAGCAACGTAGCGACTGGCCTACGTTCGGCCAAGGGACCAAGCTGGAAATCAAGCGAACTGTGG6670.11959985153121878No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6650.11924123128674735No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6610.11852399079780453No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA6580.11798606043109738No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6440.11547571871979745No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6410.11493778835309032No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6340.11368261749744035No Hit
GCCAAGGGACCAAGCTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTC6320.11332399725296892No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGTCTGGTACCAGCACAAACCTGGCCAGG6290.11278606688626179No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6280.1126067567640261No Hit
GTCTGGTACCAGCACAAACCTGGCCAGGCTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCCACTGGCATC6250.11206882639731895No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6130.10991710493049042No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6100.1093791745637833No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGGTACC6080.10902055431931189No Hit
GGTACCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAACGTAGCGACTGGCCTACGTTCGGCCAAGGGA6070.10884124419707616No Hit
CTAGGTACCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCC5980.10722745309695479No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5950.10668952273024765No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC5900.10579297211906909No Hit
CTCCCAGGCTCCTCGTCTATGATGCATCCAACAGGGCCACTGGCATCCCA5870.10525504175236196No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTC5870.10525504175236196No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC5780.10364125065224056No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5710.10238607979659059No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTT5660.10148952918541206No Hit
CAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGGTA5650.10131021906317633No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCACCAACTTAGTCTGGTACCAGCACAAAC5640.10113090894094062No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC5580.10005504820752636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGG100.008919045134.2726670
TACGTCC150.002336034669.1400441
ACGGGAC401.3144017E-859.6454516
ATTAAGT350.004831762557.5454370
TCTAGAT350.004831762557.5454370
CGAATGA200.007005982652.3937449
TGATCCG200.007724769451.11555
GGTATCA4900.044.524711
GTGAGCG1803.6379788E-1241.02775670
GTCCTTA350.00143207138.9416549
GTATCAA12450.037.511851
CTAGTTA400.002425988434.97739850
CGGGACT501.9926627E-434.0984117
GTGCTAG400.002752167934.0892331
TATCAAC14100.033.113252
TCAACGC13950.032.9777454
ATCAACG14050.032.7400933
ACGGGGC1057.3123374E-1032.4601116
CAACGCA14550.031.617845
AACGCAG14650.031.4020166
Produced by FastQC (version 0.11.5)