FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072426
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGGCTCTTATGTCTT97990.4728274978213939No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG95890.4626944460260583No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTACGACGGTGACCCGGGTCCCA71090.3430279295859056No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT68940.3326536146525859No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC59830.2886954709118685No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57160.27581201934351335No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG54280.26191526259562464No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTG52720.2543878526905183No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC50070.2416009063773568No Hit
GGACTGAACAGAGAGAACTCAACATGGAGTTTGGGCTGAAGTGGCTATTT46990.22673909707753137No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT43990.21226330879848063No Hit
GCCTTGGGCTGACCTACGACGGTGACCCGGGTCCCACTTCCGAAGACATA42180.20352958320345335No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC40760.19667771008470267No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT39690.1915146789318412No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA38730.186882426682545No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT38460.18557960573743043No Hit
CCCCTGAGGGACGCTGATTATTACTATAGATGACGACTTTGGGGGCCGTTCCTGGGACGTGTTTGTACCAGTGTA37860.1826844480816203No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC37820.18249143757123296No Hit
CCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT37730.18205716392286142No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37140.17921025889464812No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG35370.17066954381000815No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTACGACGGTGACCCGGGTCCCACTTCCGAAGACATAAGAGCCA35270.1701870175340398No Hit
GCCTTATATTTCTGTGCGAAGGATGGATCCCAGTGTGTTGGTGGGAGTTG35160.16965623863047463No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG34430.16613379681590562No Hit
GATTATTACTATAGATGACGACTTTGGGGGCCGTTCCTGGGACGTGTTTG34010.1641071864568385No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC34000.16405893382924167No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC33920.163672912808467No Hit
GGATGACAGTCTGAGTGGCTCTTATGTCTTCGGAAGTGGGACCCGGGTCACCGTCGTAGGTCAGCCCAAGGCCAA33630.16227358660815874No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT32500.1568210396897163No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG31790.15339510313034097No Hit
GTAATAATCAGCGTCCCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAG30550.14741177730833332No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG30170.14557817745965357No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA29820.14388933549376431No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG29520.14244175666585923No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGC29170.14075291469996998No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG28720.13858154645811235No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGCCTTCCCTCTCCTCCTCACCCTCCTCACTCA28390.1369892097474168No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA28160.13587939931268958No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC28070.13544512566431804No Hit
GGGTTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCAGCCATTAGTATCCTAGGTACTACGACATA27920.13472133625036553No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC27290.13168142071176486No Hit
GACCTACGACGGTGACCCGGGTCCCACTTCCGAAGACATAAGAGCCACTC26930.12994432611827877No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTGCTCTGG26780.12922053670432623No Hit
ACGACATACTATGCGGAGTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATCTG25890.12492605284820785No Hit
GAGTAGCAACTCCCACCAACACACTGGGATCCATCCTTCGCACAGAAATATAAGGCCGTGTCCTCGGCTCTGAGG25730.12415401080665847No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC25550.12328546350991543No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25530.12318895825472176No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGCCTTCCCTCTCCT25220.12169312679921986No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT24970.12048681110929896No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24800.11966651644015275No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTG24750.11942525330216856No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC24720.11928049541937806No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT24410.11778466396387616No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC24410.11778466396387616No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGATTCGGTC24390.11768815870868247No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC24320.11735039031550464No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24140.11648184301876159No Hit
ATTCAGGACAATCTCCAGCATGGCCGCCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC24140.11648184301876159No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC24050.11604756937039007No Hit
GCCCTGGGTTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTC23900.11532377995643753No Hit
GGATGGATCCCAGTGTGTTGGTGGGAGTTGCTACTCCATGTGGAACTACT23480.11329716959737042No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA23290.11238036967303054No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT23280.11233211704543371No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23230.11209085390744952No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGGCT23140.111656580259078No Hit
GGTACAAACACGTCCCAGGAACGGCCCCCAAAGTCGTCATCTATAGTAAT23100.11146356974869066No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG23090.11141531712109383No Hit
ATATAAGGCCGTGTCCTCGGCTCTGAGGCTGTCCATTTGCAGATACAGGG22870.11035375931396343No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG22770.10987123303799508No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC22660.10934045413442989No Hit
CTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG22650.10929220150683305No Hit
GTTTGTACCAGTGTACATAATTATCTCCGATGTTGGAGCTGCTTCCAGAG22590.10900268574125203No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG22430.10823064369970266No Hit
GCATGGGATGACAGTCTGAGTGGCTCTTATGTCTTCGGAAGTGGGACCCG22380.10798938056171849No Hit
TCGTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22370.10794112793412165No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATCGGAGATAATTATGTACACTGGTACAAACACGTCCCAGGAACGGCCCC22310.10765161216854063No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG22100.10663830698900709No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG22050.1063970438510229No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21890.10562500180947353No Hit
GCTCTGAGGCTGTCCATTTGCAGATACAGGGTGTTCTTGGAATTGTCTCT21860.10548024392668302No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT21480.10364664407800327No Hit
GTATGTCGTAGTACCTAGGATACTAATGGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACC21440.10345363356761592No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT21440.10345363356761592No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG21390.10321237042963174No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC21100.10181304422932351No Hit
CATAAGAGCCACTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCC21030.10147527583614566No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT20900.10084799167738678No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC20880.10075148642219312No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20810.10041371802901526No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC20800.10036546540141843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4750.047.7503270
GGTATCA17700.043.5150571
CGTATGC4150.036.61774441
CGACGTC4800.036.27431530
GTGGTAT5300.036.0094531
TCGACGT4750.035.92523629
TCGTATG4150.035.7374540
ACTCGAC4800.035.51839427
ATCTCGT3500.035.33125337
CTCGACG4850.035.16411228
GCCGTCT4250.035.1576446
CCCACGA5050.035.0782719
CGAGACT5000.034.75443323
ACGAGAC5100.034.7368822
TCTCGTA3400.034.3891538
TATACAC5200.034.0705073
GTATCAA38100.033.8120271
AGACTCG5050.033.7421325
ATGCCGT4450.033.4942744
ACGTCAT4900.033.49237432
Produced by FastQC (version 0.11.5)