FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072426
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGGCTCTTATGTCTT92180.4447927211876323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68430.33019273064514726No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC64690.3121462479239307No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT64090.30925109026812053No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTACGACGGTGACCCGGGTCCCA61380.296174628189378No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTG60600.2924109232368249No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC52630.2539535790421467No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48230.232722422899539No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG48210.23262591764434534No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT47690.23011678100930988No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG43970.21216680354328696No Hit
GGACTGAACAGAGAGAACTCAACATGGAGTTTGGGCTGAAGTGGCTATTT42510.20512191991414894No Hit
CCCCTGAGGGACGCTGATTATTACTATAGATGACGACTTTGGGGGCCGTTCCTGGGACGTGTTTGTACCAGTGTA39280.18953632120037098No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC38980.1880887423724659No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC38790.18717194244812602No Hit
CCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT38680.1866411635445608No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38360.1850970794614621No Hit
GCCTTGGGCTGACCTACGACGGTGACCCGGGTCCCACTTCCGAAGACATA37840.18258794282642662No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGCCTTCCCTCTCCTCCTCACCCTCCTCACTCA36730.17723190116317786No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC36090.17414373299698033No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35870.17308217518984997No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG35830.17288916467946264No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC35520.17139333322396072No Hit
GCCTTATATTTCTGTGCGAAGGATGGATCCCAGTGTGTTGGTGGGAGTTG35280.17023527016163664No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT34660.16724360725063284No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTACGACGGTGACCCGGGTCCCACTTCCGAAGACATAAGAGCCA33880.16347990229807965No Hit
GTAATAATCAGCGTCCCTCAGGGGTCCCTGACCGAATCTCTGGCTCCAAG33390.1611155235458347No Hit
GGATGACAGTCTGAGTGGCTCTTATGTCTTCGGAAGTGGGACCCGGGTCACCGTCGTAGGTCAGCCCAAGGCCAA33020.15933017632475177No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32700.15778609224165302No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG32460.15662802917932897No Hit
GATTATTACTATAGATGACGACTTTGGGGGCCGTTCCTGGGACGTGTTTG32200.15537346086181122No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGC32040.15460141882026185No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA31600.15247830320600106No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC30790.14856984037065737No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30340.14639847212879975No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG29540.14253826192105293No Hit
GGGTTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCAGCCATTAGTATCCTAGGTACTACGACATA29260.14118718834834149No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG28890.13940184112725856No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28430.13718222025780413No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCCTGCTCTGG27550.13293598902928258No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGCCTTCCCTCTCCT27390.13216394698773323No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA27350.13197093647734587No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC26220.12651838955890343No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT26140.12613236853812876No Hit
ATTCAGGACAATCTCCAGCATGGCCGCCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC25100.12111409526805782No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24860.11995603220573377No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATCGGAGATAATTATGTACACTGGTACAAACACGTCCCAGGAACGGCCCC24760.1194735059297654No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC24510.11826719023984451No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGGCT24430.11788116921906983No Hit
GAGTAGCAACTCCCACCAACACACTGGGATCCATCCTTCGCACAGAAATATAAGGCCGTGTCCTCGGCTCTGAGG24360.11754340082589197No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG23990.11575805360480905No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23910.11537203258403436No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT23870.11517902207364702No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23740.11455173791488815No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC23720.11445523265969448No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23690.11431047477690398No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGCCTTCCCTCTCCTC23390.1128628959489989No Hit
GGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATCGGAGATAATTAT23190.11189784339706219No Hit
GGATGGATCCCAGTGTGTTGGTGGGAGTTGCTACTCCATGTGGAACTACT23170.11180133814186852No Hit
ACGACATACTATGCGGAGTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACCCTGTATCTG23040.11117405398310964No Hit
GTTTGTACCAGTGTACATAATTATCTCCGATGTTGGAGCTGCTTCCAGAG23040.11117405398310964No Hit
ATTATGTACACTGGTACAAACACGTCCCAGGAACGGCCCCCAAAGTCGTCATCTATAGTAATAATCAGCGTCCCT22780.10991948566559191No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG22780.10991948566559191No Hit
GTGTGAGGAGGAGTTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGGGGTCCCTGAGACTTTCCTGTTCAGG22740.10972647515520458No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22690.10948521201722039No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC22670.10938870676202672No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC22580.1089544331136552No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA22250.10736209640295961No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC22050.1063970438510229No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTG21560.10403266509877795No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG21490.1036948967056001No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG21460.1035501388228096No Hit
GGTACAAACACGTCCCAGGAACGGCCCCCAAAGTCGTCATCTATAGTAAT21420.10335712831242225No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGATTCGGTC21270.10263333889846972No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC21240.1024885810156792No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT21200.10229557050529187No Hit
GGTACAAACACGTCCCAGGAACGGCCCCCAAAGTCGTCATCTATAGTAATAATCAGCGTCCCTCAGGGGTCC21120.10190954948451718No Hit
GACCTACGACGGTGACCCGGGTCCCACTTCCGAAGACATAAGAGCCACTC20940.10104100218777413No Hit
GTATGTCGTAGTACCTAGGATACTAATGGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAACC20810.10041371802901526No Hit
GCTCTGAGGCTGTCCATTTGCAGATACAGGGTGTTCTTGGAATTGTCTCT20780.10026896014622476No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG20740.10007594963583742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)