FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473464
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTCCGAAGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT44330.300855670718796No Hit
GTTTTATACAGCCCCATCAATAAGAACTACTTAGCTTGGTACCAGCACAA40370.2739802261880847No Hit
CTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT37910.2572848742826428No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC34490.2340742630970285No Hit
GCCTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGATGGGGCTGTATAAAACACT33190.22525151615512834No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30630.2078774914080018No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30390.20624867658795873No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGCGGCCGTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA27690.18792450986247375No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25160.17075408696785263No Hit
AACCAGGACAGCCTCCTAGGCTGCTCATTTACTGGGCATCTACCCGGGAA24250.1645781641085225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20970.14231769490126667No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20910.1419104911962559No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20830.14136755292290817No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20250.13743125044113735No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT18880.12813343251005793No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18850.12792983065755256No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18280.12406139545995017No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTCCGAAGACGTTCGGCCA17980.12202537693489628No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17810.1208716331040324No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17770.12060016396735855No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGATTGTTCATTTGCAGATACA16630.11286329357215379No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16480.11184528430962684No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16130.10946992936373062No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16110.10933419479539372No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC15620.10600869787113903No Hit
GTAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA15100.10247959909437897No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14820.10057931513766201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT453.137393E-853.4282639
TCGTATG3800.049.80867840
CGTATGC4250.047.85813541
ATCGCGT606.02995E-945.6286837
GGTATCA17550.044.829241
CCCACGA5400.043.1501319
GCGTATG651.1643351E-842.3547940
GTGGTAT4200.041.8058131
TATACAC5750.040.5194783
ATCTCGT3900.040.3638337
GATCGCG857.2759576E-1140.2177236
TCGCGTA603.0674164E-739.9828638
CGAGACA5800.039.60681523
CCGTCTT4900.039.1773147
TGGTATC4500.039.0187572
ATCTCCG6000.038.8285310
CGTCGAG350.001498658438.5873617
ACGAGAC6050.037.96243722
CTTATAC6150.037.8840681
TATGCCG5300.037.88387343
Produced by FastQC (version 0.11.5)