FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447935
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTAACTCACCTCCGTTCACTTTTGGCCAGGGGACCAGGCTGGAGATCAGACGAA84170.581310625131653No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56230.3883461619478775No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56100.3874483315894705No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA47220.32611961172290194No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38630.2667937441943181No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34640.23923725857859643No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA34010.23488623453400878No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33300.22998269949963224No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32600.2251482283389793No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32430.22397414248567787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30990.21402894466947758No Hit
GAGTATTAGCAGCCGTTTCTTAGCCTGGTACCAGCACAAACCTGGCCAGG30470.2104376232358497No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30240.20884915414020655No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAAGTACA29650.2047743855905134No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28900.19959459506124236No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28820.1990420840714535No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28620.1976608065969812No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28340.19572701813272003No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGACTGGAGCCTGAAGATT28220.19489825164803667No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA27420.1893731417501476No Hit
GTATGGTAACTCACCTCCGTTCACTTTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCACCAT26480.18288113762012798No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25580.17666538898500278No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25040.17293593980392766No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCCGTTTCTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAG23250.16057350640740087No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT22820.1576037598372855No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22420.15484120488834097No Hit
GCACAAACCTGGCCAGGCTCCCAGCCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT21510.14855639237949217No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG21380.14765856202108518No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21150.1460700929254421No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCCTGGTCC20730.14316941022905033No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20660.14268596311298504No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20190.1394399610479752No Hit
CCATAGATGAGGAGGCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGAAACGGCTGCTAATACTCTGA19950.13778242807860852No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC19500.1346745537610459No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19040.1314976155697597No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18720.12928757161060409No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18620.12859693287336793No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18510.12783723026240817No Hit
GTGTATTATTGTCAGCAGTATGGTAACTCACCTCCGTTCACTTTTGGCCA18490.12769910251496097No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCACCA18400.12707752765144845No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18350.12673220828283036No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17610.12162148162728299No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGAACGGAGGTGAGTTACCATACTG17540.12113803451121771No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17240.1190661182995093No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17030.11761577695131341No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16370.11305756128555496No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16210.11195253930597712No Hit
GTGTATTACTGTGCGAGAGACGGCAGCCCCAGCTTCGACTACTACTACAT16130.11140002831618824No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15910.10988062309426874No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT15790.10905185660958537No Hit
GACTTCACTCTCACTATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTATGGTAACTCA15790.10905185660958537No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15730.1086374733672437No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15550.10739432364021866No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15330.10587491841829917No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15300.10566772679712833No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15260.10539147130223386No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15240.10525334355478665No Hit
GTTACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATAGTGAGAGTGAAGT15160.10470083256499774No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15090.10421738544893246No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC15070.10407925770148521No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15040.10387206608031439No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15020.10373393833286715No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15000.10359581058541993No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14960.10331955509052548No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14920.10304329959563102No Hit
GGTTTGTGCTGGTACCAGGCTAAGAAACGGCTGCTAATACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC14880.10276704410073656No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14860.10262891635328936No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14770.10200734148977683No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14770.10200734148977683No Hit
GGCCAAAAGTGAACGGAGGTGAGTTACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTG14740.10180014986860599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)