FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447935
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTAACTCACCTCCGTTCACTTTTGGCCAGGGGACCAGGCTGGAGATCAGACGAA81910.5657021896701164No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55910.3861361179887219No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49750.34359277177497605No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45200.31216870923073203No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA44410.3067126632065666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40460.27943243308573934No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33000.22791078328792386No Hit
GAGTATTAGCAGCCGTTTCTTAGCCTGGTACCAGCACAAACCTGGCCAGG32510.22452665347546677No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32240.22266192888492922No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31870.22010656555715552No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31600.21824184096661797No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30250.20891821801393018No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29720.2052578327065787No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGACTGGAGCCTGAAGATT28610.19759174272325758No Hit
GTAATACACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAAGTACA27920.19282633543632827No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27880.19255007994143383No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26930.18598901193769057No Hit
GTATGGTAACTCACCTCCGTTCACTTTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCACCAT26200.1809473491558668No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25350.17507691988935967No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25340.17500785601563607No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25020.17279781205648043No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA24830.17148559845573177No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24180.16699644666369692No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24020.1658914246841191No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCAGCCGTTTCTTAGCCTGGTACCAGCACAAACCTGGCCAGGCTCCCAG22730.156982184973773No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22150.15297648029780342No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT21460.1482110730108741No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21390.1477276258948088No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21380.14765856202108518No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21030.14524132644075874No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20160.13923276942680438No Hit
GCACAAACCTGGCCAGGCTCCCAGCCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT20150.13916370555308077No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGCAG20120.13895651393190991No Hit
CCATAGATGAGGAGGCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGAAACGGCTGCTAATACTCTGA19240.13287889304423195No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCCTGGTCC18980.131083232327418No Hit
GTGTATTATTGTCAGCAGTATGGTAACTCACCTCCGTTCACTTTTGGCCA18800.12984008260039295No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18700.12914944386315683No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC18390.12700846377772482No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAGACGAACTGTGGCTGCACCA17890.12355527009154416No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17800.12293369522803163No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17680.12210492874334829No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17650.12189773712217744No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16820.11616543560311755No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16770.11582011623449949No Hit
GTGTATTACTGTGCGAGAGACGGCAGCCCCAGCTTCGACTACTACTACAT16610.11471509425492166No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGAACGGAGGTGAGTTACCATACTG16430.11347194452789662No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16170.11167628381108267No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16120.11133096444246461No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16100.1111928366950174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16090.11112377282129378No Hit
GGTTTGTGCTGGTACCAGGCTAAGAAACGGCTGCTAATACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC15860.10953530372565068No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15830.10932811210447983No Hit
GACTTCACTCTCACTATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTATGGTAACTCA15750.10877560111469092No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15430.1065655571555353No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15180.10483896031244497No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15100.10428644932265606No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15000.10359581058541993No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14960.10331955509052548No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14900.10290517184818379No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14880.10276704410073656No Hit
GGCCAAAAGTGAACGGAGGTGAGTTACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTG14860.10262891635328936No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14770.10200734148977683No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14670.10131670275254068No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14660.10124763887881708No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14610.10090231951019901No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14530.10034980852041009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)