FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637916
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25780.40412844324331104No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25490.39958239015795183No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT25300.39660394158478546No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19930.31242357928003056No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19480.3053693589751629No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA18120.2840499376093404No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16880.2646116416581494No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16780.26304403714595653No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16650.26100615128010585No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCACA16480.25834122360937806No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16360.25646009819474663No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16320.25583305638986953No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16220.2542654518776767No Hit
CAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCCTC16000.2508167219508525No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14730.23090814464600354No Hit
CAATATAATAGCTATTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT14410.2258918102069865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14330.22463772659723222No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13580.21288069275578603No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGA13000.20378858658506765No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12820.20096689846312052No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12740.19971281485336628No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12740.19971281485336628No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12330.19328563635337567No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12230.19171803184118286No Hit
GTCTCCTTCCGCCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCACAGTATTAGTAG11980.18779902056070077No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11670.18293944657290304No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA11400.17870691438998237No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11350.17792311213388595No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11300.17713930987778956No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11290.17698254942657027No Hit
CTACTAATACTGTGACTGGCCCGGCAAGTGATGGTGATTCTGTCTCCTACAGATGCAGACAGGGCGGAAGGAGA11260.17651226807291243No Hit
ATTATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC11250.17635550762169314No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11230.17604198671925456No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11060.17337705904852677No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAATAG10930.1713391731826761No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG10300.16146326475586129No Hit
ATATAATAGCTATTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT10280.1611497438534227No Hit
CTAATACTGTGACTGGCCCGGCAAGTGATGGTGATTCTGTCTCCTACAGA10100.15832805573147563No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCGCC9870.15472256535343212No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9870.15472256535343212No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9820.1539387630973357No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9740.15268467948758144No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9690.15190087723148502No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9670.15158735632904646No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9490.14876566820709936No Hit
ACCTTGATGGGACCCCACTTTCCAAACTGGAGGCATCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG9420.14766834504856438No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9410.1475115845973451No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9390.14719806369490654No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA9360.1467277823412487No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9320.14610074053637156No Hit
ACTTATTACTGCCAACAATATAATAGCTATTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCA9240.1448466569266173No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9230.144689896475398No Hit
CCTTCCGCCCTGTCTGCATCTGTAGGAGACAGAATCACCATCACTTGCCG8670.1359113112071182No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC8630.13528426940224103No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG8620.13512750895102177No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8560.13418694624370608No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8440.1323058208290747No Hit
GTAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCACAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA8400.13167877902419753No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8380.13136525812175898No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8170.12807328864615403No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA8140.12760300729249618No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8120.1272894863900576No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8080.1266624445851805No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8030.12587864232908408No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7990.12525160052420695No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7920.12415427736567196No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7810.12242991240225985No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC7560.1185109011217778No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7540.11819738021933922No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT7540.11819738021933922No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7450.11678653615836568No Hit
CTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT7410.11615949435348853No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG7390.11584597345105No Hit
CCCCTAAACTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCA7360.11537569209739214No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7320.11474865029251499No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7230.11333780623154145No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7210.11302428532910289No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7090.11114315991447149No Hit
GTCACAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATG7070.11082963901203294No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGATGCCTCCAGTTTGGAAAGTGGGGTCCCAT6850.10738090908520871No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6840.10722414863398942No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6830.10706738818277015No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6770.10612682547545445No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6680.10471598141448091No Hit
GTATCAACGCAGAGTACGGGAGTCAGACTCAGTCAGGACACAGCATGGAC6620.10377541870716521No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC6590.10330513735350737No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC6580.10314837690228806No Hit
TCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC6580.10314837690228806No Hit
GTCTATTACTGTGCGAGAGCAGGGGGGGATAGGAGCAGCTGGTTCCCTTT6480.10158077239009525No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6470.10142401193887597No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6460.10126725148765668No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC6440.1009537305852181No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA6400.10032668878034098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAT150.002370740568.8845739
GTGGTAT1350.063.05711
GCGTATG309.130263E-657.49040240
CGTATGC1200.051.77836641
TAGCGTC200.00772000151.12433224
CCATTGT400.00823149550.2956770
ATGCCGT1250.049.91745444
AGCGATG552.7794158E-949.7938833
ATGGTTA354.3298939E-449.64805669
GAGCGAT1450.049.5441232
TGAGCGA1550.048.52036731
TCGTATG950.047.2026540
TGCCGTC1350.046.2863545
GTATGCC1500.046.08013542
ACACATC1800.045.401116
TATACCT306.7301723E-445.4011085
CGAGACA1750.044.78411523
AGCGATC1000.044.50328433
CACGAGA1850.044.198321
ACTGAGC1700.044.18729
Produced by FastQC (version 0.11.5)