FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212417
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41570.34286883143340946No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41560.3427863515605604No Hit
GTCTACAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA39620.32678525622784904No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTG31830.26253343527845613No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29210.24092370859201084No Hit
GTTTGTAGACCTTGCATGCAGTAATAAGCCCCAACATCCTCAGTCTCCACTTTGCTGATTTTCAGTGTAAAAT27180.22418029440365814No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26900.22187085796388537No Hit
GGTCTAGTCAGAGCCTCGTGGGTACTAATGGCTACAACTTTTTGAATTGGTACATACAAAAACCAGGGCAGTCTC26480.21840670330422618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25520.21048863551071947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25520.21048863551071947No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25470.21007623614647436No Hit
GTAATATACGCCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGTAGAAACA24650.20331288657285404No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24090.1986940136933085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23740.19580721814359253No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22460.18524979441891692No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22180.18294035797914415No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21850.1802185221751262No Hit
GTAATAAGCCCCAACATCCTCAGTCTCCACTTTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA21630.1784039649724476No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21520.1774966863711083No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21270.1754346895498826No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21240.1751872499313355No Hit
GATTTTACACTGAAAATCAGCAAAGTGGAGACTGAGGATGTTGGGGCTTATTACTGCATGCAAGGTCTACAAACT20790.17147565565312925No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20710.17081581667033702No Hit
GTGTTATATTCAGCTGTTGGAGTCTGGGGGAGGCTTGGTTCAGCCTGGGGAGTCCCTGAGACTCTCCTGTGAAGC19830.16355758785962254No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18350.1513505666779664No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18040.14879369061964653No Hit
GAGTTTGTAGACCTTGCATGCAGTAATAAGCCCCAACATCCTCAGTCTCCACTTTGCTGATTTTCAGTGTAAAAT17930.14788641201830724No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17550.14475217685004416No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17360.14318505926591263No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17360.14318505926591263No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGACCTGGCTTTTT17080.14087562282613986No Hit
AGTCAGAGCCTCGTGGGTACTAATGGCTACAACTTTTTGAATTGGTACATACAAAAACCAGGGCAGTCTCCACAA16980.14005082409764957No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16630.13716402854793358No Hit
CTACAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG16330.13468963236246276No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16270.13419475312536858No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16010.13205027643129386No Hit
GTACTAATGGCTACAACTTTTTGAATTGGTACATACAAAAACCAGGGCAG15930.13139043744850162No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15540.12817372240738953No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCT15460.12751388342459732No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15380.1268540444418051No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15020.12388476901924007No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14950.1233074099092969No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14870.12264757092650465No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14640.12075053385097702No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14610.12050309423242993No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14430.11901845652114744No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGA14420.11893597664829839No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT14280.11778125842841201No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14180.11695645969992173No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT14120.11646158046282756No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14020.11563678173433728No Hit
CTCCACAACTCCTGATCTTTTTGGGTTCTAATCGGGTCCCAGGGGTCCCT13790.11373974465880965No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTGGGTACTAATGG13700.11299742580316839No Hit
AAGTAGTAGTAGTGGTTATGATGTAGCCTACGCAGGCTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAACTC13700.11299742580316839No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13610.11225510694752713No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13550.11176022771043295No Hit
GTATATTACTGTGCGAGCGGGGGCACTGTTGGGCGTGTTAGAAAGATCTA13530.1115952679647349No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAAAG13450.11093542898194268No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13300.10969823088920726No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13260.10936831139781114No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13180.10870847241501894No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA13070.10780119381367961No Hit
CAGTAATATACGCCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGTAGAAA12720.10491439826396363No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12670.1045019988997185No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12660.10441951902686947No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12650.10433703915402044No Hit
GTAGTAGTAGTGGTTATGATGTAGCCTACGCAGGCTCCGTGAAGGGCCGG12560.10359472029837918No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12550.10351224042553016No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12500.10309984106128502No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12480.10293488131558697No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12400.10227504233279473No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12370.10202760271424766No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12310.10153272347715347No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACTCTCC12280.1012852838586064No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12270.10120280398575737No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12270.10120280398575737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)