FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212417
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40920.3375076396982227No Hit
GTCTACAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA38520.3177124702144559No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35950.2965151428922557No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35020.2888445147172961No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30840.25436792786640244No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27180.22418029440365814No Hit
GTTTGTAGACCTTGCATGCAGTAATAAGCCCCAACATCCTCAGTCTCCACTTTGCTGATTTTCAGTGTAAAAT27140.22385037491226203No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTG26730.2204687001254519No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25480.2101587160193234No Hit
GGTCTAGTCAGAGCCTCGTGGGTACTAATGGCTACAACTTTTTGAATTGGTACATACAAAAACCAGGGCAGTCTC25300.20867407830804088No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23550.19424010055946098No Hit
GTAATATACGCCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGTAGAAACA23200.19135330500974498No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23100.1905285062812547No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22740.1875592308586897No Hit
GTGTTATATTCAGCTGTTGGAGTCTGGGGGAGGCTTGGTTCAGCCTGGGGAGTCCCTGAGACTCTCCTGTGAAGC22540.18590963340170916No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21540.17766164611680635No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21290.17559964929558064No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21090.1739500518386001No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21010.17329021285580787No Hit
GTAATAAGCCCCAACATCCTCAGTCTCCACTTTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA20930.17263037387301564No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19320.15935111434432211No Hit
GATTTTACACTGAAAATCAGCAAAGTGGAGACTGAGGATGTTGGGGCTTATTACTGCATGCAAGGTCTACAAACT19100.1575365571416435No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18940.15621687917605906No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGACCTGGCTTTTT18450.15217536540645668No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18410.15184544591506058No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18290.15085568744087224No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18140.14961848934813682No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18130.14953600947528778No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17870.14739153278121306No Hit
GAGTTTGTAGACCTTGCATGCAGTAATAAGCCCCAACATCCTCAGTCTCCACTTTGCTGATTTTCAGTGTAAAAT17450.1439273781215539No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17300.14269018002881848No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17070.14079314295329082No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16640.13724650842078262No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16540.13642170969229234No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15760.12998827961006815No Hit
GTACTAATGGCTACAACTTTTTGAATTGGTACATACAAAAACCAGGGCAG15130.12479204762057938No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15030.1239672488920891No Hit
AGTCAGAGCCTCGTGGGTACTAATGGCTACAACTTTTTGAATTGGTACATACAAAAACCAGGGCAGTCTCCACAA14810.12215269168941048No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14720.12141037283376925No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCT14670.1209979734695241No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14240.1174513389370159No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14200.11712141944561977No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT14160.11679149995422367No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14150.11670902008137464No Hit
CTACAAACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG14050.11588422135288437No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGA13960.11514190249724311No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTGGGTACTAATGG13950.11505942262439409No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13860.11431710376875283No Hit
CTCCACAACTCCTGATCTTTTTGGGTTCTAATCGGGTCCCAGGGGTCCCT13670.1127499861846213No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13590.11209014720182907No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13430.11077046923624462No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13280.1095332711435092No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13140.1083785529236228No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACTCTCC13100.1080486334322267No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGAGATATTGTGTTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC13100.1080486334322267No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12980.10705887495803836No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12890.10631655610239713No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12830.10582167686530296No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12700.10474943851826558No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG12640.10425455928117142No Hit
GTATATTACTGTGCGAGCGGGGGCACTGTTGGGCGTGTTAGAAAGATCTA12450.10268744169703989No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12440.10260496182419085No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12280.1012852838586064No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAAAG12270.10120280398575737No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12130.10004808576587099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAA200.007557175651.4035433
GGTATCA15950.040.1189841
CTAACGC350.001444172238.8787043
ACCGTTG350.001444172238.8787048
ATACCGT350.001444172238.8787046
GTATCAA36150.035.2140581
GTGGTAT4950.035.0685421
TGGTATC5600.031.594162
GTCTTAC652.5461588E-531.418881
CCTAACG450.004947486330.2439822
TACCGTT450.0049514830.2389957
TATCAAC42800.029.7316722
ACGAACC1202.21371E-929.2751152
ATCAACG43550.029.2148273
TCAACGC44300.028.8738044
CAACGCA45400.028.0992875
TGTACGT500.007145997628.0515451
AACGCAG45750.027.9586776
GAGTTTA5500.027.848551
CCTATAC1104.1987732E-827.833623
Produced by FastQC (version 0.11.5)