FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591557
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAATAGTTACCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT85090.5346336951802543No Hit
CTATTGTACTGTTGGCAGTAATAAGTTGCGAAATCATCAGGCTGCAGGCCGTTGATGGTGAGAGTGAACTCTGTC78890.4956781315403721No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66980.42084575041924355No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65990.41462542654771395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50180.31528873926601436No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43150.27111815662272853No Hit
GTAATACACAGCCGAGTCTGTGGCGGTCACAGAGGTCAGCCTCAGGGAGA42720.26841639978963994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40590.2550332787327127No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40170.25239435345388195No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39980.25120055392298235No Hit
GTCTATTATAGTGGGAGTACCTCCTACAACCCGTCCCTCAAGAGTCGAAT39480.2480589762100886No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG39470.24799614465583075No Hit
GAGTTCACTCTCACCATCAACGGCCTGCAGCCTGATGATTTCGCAACTTATTACTGCCAACAGTACAATAGTTAC37770.23731478043199206No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37700.23687495955218696No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36750.2309059618976889No Hit
GTGTATTACTGTGCGAGACATTGGTATTACGATTATTGGAGTTCTTATTA36370.22851836283588964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36370.22851836283588964No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35720.2244343118091278No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35130.2207272501079132No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33850.21268481116290525No Hit
GTCTGCATCTGTAGGAGACACCGTAACCATCACTTGCCGGGCCAGTCAGA33830.2125591480543895No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30030.1886831574363972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29830.1874265263512397No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28330.17800179321255852No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28000.17592835192204867No Hit
GACCAGGGGAAGTCCCTGAACTCCTGATCTATAAGGCGTCTAGTTTACAA27550.1731009319804443No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26910.16907971250794035No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25610.16091161045441665No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24760.1555709283424973No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24580.15443996036585558No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24020.1509213933274146No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23900.1501674146763201No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22930.1440727539133063No Hit
ACTTATTACTGCCAACAGTACAATAGTTACCCGACGTTCGGCCAAGGGAC22850.1435701014792433No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22590.14193648106853854No Hit
GTCTCTGGTGACTCCATCAGTCGAAGTGATTACTACTGGGGCTGGATCCG22560.14174798640576491No Hit
GAGTGTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGACCAGGGGAAGTCCCTGAACTCCTGATCTATAAGGCGT22310.14017719754931807No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGCCCCCA21980.1381037562588082No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21950.13791526159603457No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21820.13709845139068222No Hit
GTCAGAGTGTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGACCAGGGGAAGTCCCTGAACTCCTGATCTATAAGG21780.13684712517365072No Hit
GTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGACCAGGGGAAGTCCCTGA21640.13596748341404047No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21310.1338940421235306No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21030.13213475860431012No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20710.13012414886805812No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20680.12993565420528452No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20590.12937017021696365No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20380.12805070757754827No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20070.12610292939555418No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19950.12534895074445968No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTGCCACG19950.12534895074445968No Hit
GTGTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGACCAGGGGAAGTCCCT19940.1252861191902018No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19650.12346400411672342No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19570.12296135168266044No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19540.12277285701988681No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19000.11937995308996159No Hit
GGTATCAGCAGAGACCAGGGGAAGTCCCTGAACTCCTGATCTATAAGGCGTCTAGTTTACAAGGTGGGGGCCCAT19000.11937995308996159No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18950.11906579531867222No Hit
GTAGGAGACACCGTAACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGA18900.11875163754738284No Hit
GTATTACTGTGCGAGACATTGGTATTACGATTATTGGAGTTCTTATTATA18840.1183746482218356No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18840.1183746482218356No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18820.11824898511331985No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG18540.11648970159409935No Hit
ATAATAGACAGTCCCAAGCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA18520.1163640384855836No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18270.11479324962913676No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18220.11447909185784738No Hit
CAGTACAATAGTTACCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18100.1137251132067529No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17940.11271980833862688No Hit
CAGTAATACACAGCCGAGTCTGTGGCGGTCACAGAGGTCAGCCTCAGGGA17860.1122171559045639No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17080.10731629467244969No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17050.10712780000967606No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17040.10706496845541819No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17010.10687647379264457No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGTCGAAGTGATTACTACTGGG16930.10637382135858157No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16910.10624815825006581No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16840.10580833737026069No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16780.10543134804471344No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16770.10536851649045556No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT16710.10499152716490832No Hit
CCTACTAACACTCTGACTGGCCCGGCAAGTGATGGTTACGGTGTCTCCTACAGATGCAGACAGCGTGGCAGGAGA16530.10386055918826659No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16420.10316941209142998No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16390.10298091742865635No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16200.10178711789775671No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16100.10115880235517798No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16070.10097030769240435No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAACGGCCTGCAGCCTGATGATTTCGCAACTTATTACTGCCAACAGTA15960.10027916059556774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)