FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591557
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAATAGTTACCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT77550.4872587032698169No Hit
CTATTGTACTGTTGGCAGTAATAAGTTGCGAAATCATCAGGCTGCAGGCCGTTGATGGTGAGAGTGAACTCTGTC68760.4320297670771452No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT64710.406582987602706No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56150.35279917715796544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC50930.32000110583535496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43740.27482521832394313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42230.2653376536310041No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41030.25779786712005914No Hit
GTAATACACAGCCGAGTCTGTGGCGGTCACAGAGGTCAGCCTCAGGGAGA40200.2525828481166556No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38170.23982804260230706No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37500.23561832846702946No Hit
GAGTTCACTCTCACCATCAACGGCCTGCAGCCTGATGATTTCGCAACTTATTACTGCCAACAGTACAATAGTTAC37420.2351156760329665No Hit
GTCTATTATAGTGGGAGTACCTCCTACAACCCGTCCCTCAAGAGTCGAAT36750.2309059618976889No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT36370.22851836283588964No Hit
GTGTATTACTGTGCGAGACATTGGTATTACGATTATTGGAGTTCTTATTA35870.22537678512299592No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34970.2197219452397872No Hit
GTCTGCATCTGTAGGAGACACCGTAACCATCACTTGCCGGGCCAGTCAGA34960.21965911368552932No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34930.21947061902275572No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33530.21067420142665327No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32660.20520785620621818No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32640.20508219309770243No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30230.1899397885215547No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29480.1852274219522141No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28760.18070355004564712No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28300.1778132985497849No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27880.17517437327095417No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27430.17234695332934982No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26150.16430451438434188No Hit
GACCAGGGGAAGTCCCTGAACTCCTGATCTATAAGGCGTCTAGTTTACAA26050.16367619884176313No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24020.1509213933274146No Hit
GTCTCTGGTGACTCCATCAGTCGAAGTGATTACTACTGGGGCTGGATCCG23830.14972759379651499No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23790.14947626757948348No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23000.1445125747931114No Hit
GAGTGTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGACCAGGGGAAGTCCCTGAACTCCTGATCTATAAGGCGT22660.14237630194834366No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA22600.14199931262279641No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22590.14193648106853854No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22550.14168515485150704No Hit
GTGTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGACCAGGGGAAGTCCCT21950.13791526159603457No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGGTGAACCTTGATGGGCCCCCA21500.13508784165443022No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21430.1346480207746251No Hit
ACTTATTACTGCCAACAGTACAATAGTTACCCGACGTTCGGCCAAGGGAC21400.13445952611185147No Hit
GTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGACCAGGGGAAGTCCCTGA20630.12962149643399515No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20400.12817637068606402No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20300.12754805514348527No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19990.12560027696149118No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19960.12541178229871755No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19840.12465780364762306No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19710.12384099344227067No Hit
GTCAGAGTGTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGACCAGGGGAAGTCCCTGAACTCCTGATCTATAAGG19680.12365249877949705No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19280.12113923660918209No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19170.12044808951234545No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTGCCACG19010.11944278464421947No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18650.117180848690936No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18630.11705518558242023No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18590.11680385936538873No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18540.11648970159409935No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18460.11598704916003637No Hit
GTAGGAGACACCGTAACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAGGTGGGTGGCCTGGTATCAGCAGAGA18340.11523307050894187No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18090.11366228165249502No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17990.11303396610991626No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17800.11184016657901664No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17610.11064636704811703No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17510.1100180515055383No Hit
GTGGGGGCCCATCAAGGTTCACCGGCAGTGGATCTGGGACAGAGTTCACT17420.10945256751721741No Hit
GTATTACTGTGCGAGACATTGGTATTACGATTATTGGAGTTCTTATTATA17280.10857292575760717No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17230.1082587679863178No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT17230.1082587679863178No Hit
CAGTACAATAGTTACCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17140.10769328399799694No Hit
ATAATAGACAGTCCCAAGCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA17070.10725346311819181No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17040.10706496845541819No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16780.10543134804471344No Hit
GGTATCAGCAGAGACCAGGGGAAGTCCCTGAACTCCTGATCTATAAGGCGTCTAGTTTACAAGGTGGGGGCCCAT16740.10518002182768195No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16650.10461453783936107No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16490.10360923297123509No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16440.10329507519994573No Hit
CAGTAATACACAGCCGAGTCTGTGGCGGTCACAGAGGTCAGCCTCAGGGA16390.10298091742865635No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16330.10260392810310909No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16310.10247826499459334No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16150.10147296012646735No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16110.10122163390943585No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16020.10065614992111499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATT6900.054.204751
TCTATTA7650.050.206582
CTATTAT9100.041.451793
GTGGTAT4600.041.392721
TGCGGAG13800.040.4719270
GACAGGT4000.039.9513661
ATAGTGG9800.038.8364948
GAGTACC9850.038.30880715
GGGAGTA10000.037.37895213
GTACCTC10100.037.03213517
GGAGTAC10500.035.9237614
TACCTCC10600.035.6139518
TAGTGGG11100.034.288089
GTATCAA40900.033.6686331
CCTCCTA11950.031.61546920
GTGGGAG12250.030.79179211
TATTACA16350.029.33722770
TCGAATC13300.029.02756545
TATCAAC47350.028.8569012
ACCTCCT13250.028.75145119
Produced by FastQC (version 0.11.5)