FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889116
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21250.23900143513332345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19300.21706953873285376No Hit
GTCTCAGTCAGGACACAACATGGACATGAGGGTCCCCGCTCAGCTCCTGG17900.20132356182995242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17750.19963649287607016No Hit
GTAATACACAGCCGTGTCATCGTCTCTCAGACTGTTCATTTGCAGATACA16760.1885018377804471No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14580.1639831023173579No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13230.14879948173241736No Hit
GTGTATTACTGTGTGAGAGAGGGTAGGAGAGAAGTGGCCCCCGATGCCTT13220.1486870104688252No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13140.147787240360088No Hit
GATCTATGCTGCGTCCACTTTGCAGTATGGGGTCTCATCAAGATTTAGTGGCAGTGGGTCTGGGAGAGACTTCAC13000.14621264266979786No Hit
GGACACAACATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12980.14598770014261356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12790.14385074613436266No Hit
GCATAGATCAGGAGCTTAGGGGCTTTACCCGGTGTGTGCTGATACCAATT12610.14182626338970392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12380.13923942432708444No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12380.13923942432708444No Hit
CAACATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTAGTCTGGCTCCGAGGTGCCAGATGTGACAT12120.13631517147368846No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12000.13496551631058265No Hit
GTATGGGGTCTCATCAAGATTTAGTGGCAGTGGGTCTGGGAGAGACTTCACCCTCACCATCTTCAATCTACAACC11790.13260361977514745No Hit
CAGTAATACACAGCCGTGTCATCGTCTCTCAGACTGTTCATTTGCAGATA11760.132266205984371No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11750.13215373472077885No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11520.12956689565815935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11330.12742994164990845No Hit
CAGTACCCCTATCACCTTCGGCCAAGGGACACGACTGCAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCAT11330.12742994164990845No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAGGTATTTAAATTGGTATCAGCACACA11030.1240558037421439No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10440.1174199991902069No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10330.11618281529069323No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9910.11145902221982283No Hit
GTCTGGGAGAGACTTCACCCTCACCATCTTCAATCTACAACCCGAAGATT9890.11123407969263854No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9790.11010936705671702No Hit
CTGCTAGTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9320.10482321766788584No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9310.10471074640429372No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9280.10437333261351725No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8980.10099919470575268No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8920.10032436712419977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTG200.00756609351.38704337
GTGGTAT2450.044.191531
GCCGTCT1000.041.79511346
CGTATGC1051.6370905E-1136.14252541
TCGTATG1051.6370905E-1136.08251640
TTTCGAT750.002146440335.85982570
ATCTCGT807.543713E-834.2580337
ATGCCGT1253.6379788E-1233.31265344
GATGCGC552.2895575E-433.3038664
TAAGGCG1750.032.9601429
GTATCAA22350.032.2446171
TATACAC1900.032.05523
ACGGGTA751.9436866E-631.57675617
GACTAAG1950.031.26492326
TATCAAC23750.030.201422
ATCAACG23750.030.0606523
TCAACGC24150.029.5627544
ACTAAGG2000.028.80113427
CAACGCA24800.028.7879225
AATGGTA3750.028.68786270
Produced by FastQC (version 0.11.5)