FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889116
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21080.23708942365225685No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20860.2346150558532295No Hit
GTCTCAGTCAGGACACAACATGGACATGAGGGTCCCCGCTCAGCTCCTGG16250.18276580333724735No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15420.1734306884590987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15030.16904430917900476No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15010.16881936665182046No Hit
GTAATACACAGCCGTGTCATCGTCTCTCAGACTGTTCATTTGCAGATACA14010.15757224029260525No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12890.14497545877028417No Hit
GGACACAACATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12890.14497545877028417No Hit
GATCTATGCTGCGTCCACTTTGCAGTATGGGGTCTCATCAAGATTTAGTGGCAGTGGGTCTGGGAGAGACTTCAC12760.1435133323435862No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12340.1387895392727158No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12300.13833965421834724No Hit
CAACATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTAGTCTGGCTCCGAGGTGCCAGATGTGACAT11950.13440315999262187No Hit
GTGTATTACTGTGTGAGAGAGGGTAGGAGAGAAGTGGCCCCCGATGCCTT11730.13192879219359452No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11600.13046666576689656No Hit
GTATGGGGTCTCATCAAGATTTAGTGGCAGTGGGTCTGGGAGAGACTTCACCCTCACCATCTTCAATCTACAACC10680.12011930951641855No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10530.11843224056253628No Hit
CAGTAATACACAGCCGTGTCATCGTCTCTCAGACTGTTCATTTGCAGATA10500.11809482677175981No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10430.11730752792661475No Hit
GCATAGATCAGGAGCTTAGGGGCTTTACCCGGTGTGTGCTGATACCAATT10340.11629528655428538No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGTAGGTATTTAAATTGGTATCAGCACACA10300.11584540149991676No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10110.11370844749166588No Hit
GGGGTACTGTGACCTTGTTGACAATAATAAATTGCAAAATCTTCGGGTTG10000.1124712635921522No Hit
CAGTACCCCTATCACCTTCGGCCAAGGGACACGACTGCAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCAT9910.11145902221982283No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9860.11089666590186208No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9680.10887218315720334No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9490.10673522914895245No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9180.10324861997759573No Hit
CTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAGTGCATGCCAAAACTTCTGAAGTTGAATCCAGACGCTGCACA9110.10246132113245067No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8960.10077425217856838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2350.037.3893131
GTATCAA20550.035.52091
TATCAAC22700.031.6956882
ATCAACG22800.031.6923393
TCAACGC23400.031.0135444
CAACGCA24300.030.00385
AACGCAG24500.029.6210986
CGATATG655.1036023E-429.04766867
ACGCAGA25700.028.2380127
CGCAGAG25850.027.9435778
AGAGTAC26200.027.43836811
CAGAGTA26550.027.079710
GCAGAGT27250.026.5079449
CCCGATA859.186139E-526.02666365
GACAGGA2250.025.5333861
CCGATAT750.00129755324.75870566
AGGTATC1001.8407749E-523.6452792
CTCGCAT1302.2652966E-723.38412717
AGTACGG21450.023.13099913
AATGGTA4054.0017767E-1122.86269670
Produced by FastQC (version 0.11.5)