FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760257
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCACACCTGGCAGTAATAGTCGGCCTCGTCCCCGGCCTCGACCCTGC89120.5062897065598944No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC80490.4572627746971039No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTTCGCCGAAGATGACATGATTAGTAGGACTATCCCACAC77940.442776253694773No Hit
GTGTGGGATAGTCCTACTAATCATGTCATCTTCGGCGAAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG69770.3963625766010304No Hit
GTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC68830.3910224472903672No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTT65240.37062769811453666No Hit
GACCTAGGACGGTCAGTTTGGTCCCTTCGCCGAAGATGACATGATTAGTA60620.3443815306514901No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT59120.3358600477089425No Hit
GGCCAGGCCCCTGTGGTGATCGTCTATGATGATACCGACCGGCCCTCAGG49020.2784820625624554No Hit
GTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACCATTGT47920.2722329750712538No Hit
GGGTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACCATTGT47800.27155125643585No Hit
CCCCAGGACAGACGGCCACCATTGTCTGTGGGGGAGACAACATTGGAAGT45330.2575192145237883No Hit
GGCCAGGCCAGGCCCCTGTGGTGATCGTCTATGATGATACCGACCGGCCC45250.25706473543351904No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACTTCCTATGTGTTGACT45150.25649663657068256No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43120.24496422965510148No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42810.24320312318030832No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41390.23513611932802994No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG41270.23445440069262613No Hit
GCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCA39800.2261033474089295No Hit
CTGTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGG39750.2258192979775112No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG39740.2257624880912276No Hit
CCCCTGTGGTGATCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCT37730.21434370094821378No Hit
GTGTACACTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGGTGATCGTC37380.21235535492828603No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTG36990.21013976936322368No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGA36920.2097421001592381No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG36230.2058222180056662No Hit
GTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36110.20514049937026244No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36050.20479964005256052No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGCGGTGGTCTCCA35540.20190233585209433No Hit
GTTTGGTCCCTTCGCCGAAGATGACATGATTAGTAGGACTATCCCACACCTGGCAGTAATAGTCGGCCTCGTCCC35530.20184552596581068No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC35240.2001980392635848No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC34620.19667582631399844No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG34090.19366490234096498No Hit
CCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGTACACTTCGACTT33900.19258551450157563No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT33850.19230146507015736No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG33370.18957459052854214No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGA33260.18894968177942198No Hit
TGATGATACCGACCGGCCCTCAGGGATCCCTGACAGATTCTCTGGCTTCATCTCTGGGGGCACGGCCTCCCTGAT33220.18872244223428739No Hit
CCTCTCTCACTGCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGCCAC32880.18679090610064325No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32510.18468894030814817No Hit
TAGTAGGACTATCCCACACCTGGCAGTAATAGTCGGCCTCGTCCCCGGCCTCGACCCTGCTGATGATCAGGGAGG32330.18366636235504247No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT32010.18184844599396566No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31560.1792920011112014No Hit
CCATTGTCTGTGGGGGAGACAACATTGGAAGTCGAAGTGTACACTGGTACCAGCAGAGGCCAGGCCAGGCCC30760.17474721020850933No Hit
GTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC30500.17327015316513442No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30420.1728156740748652No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGC30290.17207714555317774No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACTTCCTA30210.17162266646290855No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA29880.16974794021554807No Hit
CCTATGAGCGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29510.167645974423053No Hit
CTCACTGCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG29160.16565762840312523No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG29160.16565762840312523No Hit
GCCCTCAGGGATCCCTGACAGATTCTCTGGCTTCATCTCTGGGGGCACGG29020.16486228999515412No Hit
TATCAATACTGATGGGAGTATCATAGATTACGCGGACTCCGTGAAGGGCCGATTCGCCATCTCCAGAGACAACCC28660.1628171340889427No Hit
GTATCATCATAGACGATCACCACAGGGGCCTGGCCTGGCCTCTGCTGGTA28530.16207860556725523No Hit
GGATAGTCCTACTAATCATGTCATCTTCGGCGAAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC27590.15673847625659207No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAGCGTTC27500.1562271872800392No Hit
CCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG27500.1562271872800392No Hit
GGACTATCCCACACCTGGCAGTAATAGTCGGCCTCGTCCCCGGCCTCGACCCTGCTGATGATCAGGGAGGCCGTG27210.15457970057781337No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG27170.15435246103267874No Hit
GGCCTGGCCTGGCCTCTGCTGGTACCAGTGTACACTTCGACTTCCAATGTTGTCTCCCCCACAGACAATGGTGGC27130.15412522148754415No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27110.15401160171497683No Hit
CTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGA27010.15344350285214034No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC26890.15276178421673653No Hit
GTCCTACTAATCATGTCATCTTCGGCGAAGGGACCAAACTGACCGTCCTA26850.15253454467160193No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC26780.15213687546761637No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACCATTGTCTGTGGGGGAGACAACATTGGAAGTCGAAGTGTA26630.1512847271733616No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT26570.15094386785565972No Hit
ACCTAGGACGGTCAGTTTGGTCCCTTCGCCGAAGATGACATGATTAGTAG26340.14963724047113575No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25040.14225195525426115No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24700.14032041912061705No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG24590.1396955103714969No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT23830.13537795901393945No Hit
GTTGAGAACCTATGAGCGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23540.13373047231171356No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT23430.1331055635625934No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAGCGTTCTGTAGGGGC22810.12958335061300708No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA22380.1271405255028101No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACTTC22210.12617475743598802No Hit
GGACAGACGGCCACCATTGTCTGTGGGGGAGACAACATTGGAAGTCGAAG21440.12180039619214694No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA21340.12123229732931044No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21330.12117548744302678No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21280.12089143801160852No Hit
CTGCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC20900.11873266233282981No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20700.11759646460715681No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20550.11674431631290202No Hit
GTAATAGTCGGCCTCGTCCCCGGCCTCGACCCTGCTGATGATCAGGGAGG20480.11634664710891648No Hit
GGCCTGGCCTCTGCTGGTACCAGTGTACACTTCGACTTCCAATGTTGTCTCCCCCACAGACAATGGTGGCCGT20460.11623302733634919No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19900.11305167370446476No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19840.11271081438676285No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCACCATTGTCTGTGGGGGAGACAA19650.11163142654737347No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19630.11151780677480617No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC19400.11021117939028222No Hit
GTATTGATACGTGCGACCCATTCCAGCCCCTTCCCTGGAGCTTGACGGAC18700.10623448735042668No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18670.10606405769157572No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT18560.10543914894245555No Hit
GCAGAGGCCAGGCCAGGCCCCTGTGGTGATCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCTGACAGATT18500.10509828962475365No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG18420.10464381053448446No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18060.10259865462827301No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17840.10134883713003272No Hit
GTCGAGGCCGGGGACGAGGCCGACTATTACTGCCAGGTGTGGGATAGTCC17720.10066711849462892No Hit
CCCTGATCATCAGCAGGGTCGAGGCCGGGGACGAGGCCGACTATTACTGC17610.10004220974550876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)