FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760257
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGATAGTCCTACTAATCATGTCATCTTCGGCGAAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGG78440.44561674800895557No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTTCGCCGAAGATGACATGATTAGTAGGACTATCCCACAC74970.42590371746852873No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC74600.4238017516760337No Hit
ATCCCACACCTGGCAGTAATAGTCGGCCTCGTCCCCGGCCTCGACCCTGC74450.4229496033817789No Hit
GTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC66150.37579739776634885No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTT58130.3302358689668611No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56070.3185330323924291No Hit
GGGTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACCATTGT55180.31347695251318414No Hit
GACCTAGGACGGTCAGTTTGGTCCCTTCGCCGAAGATGACATGATTAGTA52460.29802466344403117No Hit
GTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACCATTGT51290.29137790674884406No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49200.27950464051556106No Hit
GGCCAGGCCCCTGTGGTGATCGTCTATGATGATACCGACCGGCCCTCAGG48570.2759256176796911No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG48440.27518708915800366No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACTTCCTATGTGTTGACT46960.26677922598802334No Hit
GGCCAGGCCAGGCCCCTGTGGTGATCGTCTATGATGATACCGACCGGCCC45540.25871222213574496No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC43590.24763429431043307No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT42130.2393400509130201No Hit
CCCCAGGACAGACGGCCACCATTGTCTGTGGGGGAGACAACATTGGAAGT41190.23399992160235694No Hit
CTGTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGG40720.23132985694702535No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG39660.22530800900095838No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39380.2237173321850162No Hit
CCCCTGTGGTGATCGTCTATGATGATACCGACCGGCCCTCAGGGATCCCT38330.21775229412523286No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG38280.2174682446938146No Hit
GCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCA38080.2163320469681416No Hit
GTGTACACTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGGTGATCGTC37960.21565032833273778No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGA37700.21417327128936287No Hit
GTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36720.2086059024335651No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC36300.2062198872096518No Hit
GTTTGGTCCCTTCGCCGAAGATGACATGATTAGTAGGACTATCCCACACCTGGCAGTAATAGTCGGCCTCGTCCC35750.203095343464051No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35460.2014478567618251No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG35100.19940270085561368No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC34160.19406257154495055No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGCGGTGGTCTCCA34070.19355128256839768No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTG33440.18997225973252768No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGC33200.1886088224617201No Hit
TGATGATACCGACCGGCCCTCAGGGATCCCTGACAGATTCTCTGGCTTCATCTCTGGGGGCACGGCCTCCCTGAT33180.18849520268915276No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT33080.18792710382631628No Hit
CCTCTCTCACTGCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGCCAC32980.18735900496347976No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACTTCCTA32950.18718857530462882No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGA32430.18423446121787898No Hit
CCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGTACACTTCGACTT32380.1839504117864607No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31550.1792351912249177No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31410.17843985281694663No Hit
GTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC31400.17838304293066296No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31370.178212613271812No Hit
CCATTGTCTGTGGGGGAGACAACATTGGAAGTCGAAGTGTACACTGGTACCAGCAGAGGCCAGGCCAGGCCC29770.16912303146642793No Hit
TAGTAGGACTATCCCACACCTGGCAGTAATAGTCGGCCTCGTCCCCGGCCTCGACCCTGCTGATGATCAGGGAGG29710.168782172148726No Hit
GTCCTACTAATCATGTCATCTTCGGCGAAGGGACCAAACTGACCGTCCTA29220.16599848772082712No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28890.16412376147346666No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28880.164066951587183No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGACTTC28600.1624762747712408No Hit
GGCCTGGCCTGGCCTCTGCTGGTACCAGTGTACACTTCGACTTCCAATGTTGTCTCCCCCACAGACAATGGTGGC28480.161794556135837No Hit
GTATCATCATAGACGATCACCACAGGGGCCTGGCCTGGCCTCTGCTGGTA28240.16043111886502937No Hit
GCCCTCAGGGATCCCTGACAGATTCTCTGGCTTCATCTCTGGGGGCACGG27910.1585563926176689No Hit
GGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG27290.15503417966808256No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAGCGTTC26740.15190963592248177No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC26630.1512847271733616No Hit
CTCACTGCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG26560.15088705796937607No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTC26410.1500349096751213No Hit
GGATAGTCCTACTAATCATGTCATCTTCGGCGAAGGGACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC26260.14918276138086653No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGT25830.14673993627066956No Hit
GGACTATCCCACACCTGGCAGTAATAGTCGGCCTCGTCCCCGGCCTCGACCCTGCTGATGATCAGGGAGGCCGTG25720.14611502752154942No Hit
TATCAATACTGATGGGAGTATCATAGATTACGCGGACTCCGTGAAGGGCCGATTCGCCATCTCCAGAGACAACCC25480.1447515902507418No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25440.1445243507056072No Hit
CCTATGAGCGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25280.14361539252506877No Hit
CTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGA24970.1418542860502756No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24960.14179747616399196No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24750.1406044685520353No Hit
CCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG24630.13992274991663148No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG24510.1392410312812277No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACCATTGTCTGTGGGGGAGACAACATTGGAAGTCGAAGTGTA24470.13901379173609307No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23850.13549157878650675No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23450.13321918333516072No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22960.13043549890726183No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG22950.13037868902097818No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22820.12964016049929072No Hit
GTTGAGAACCTATGAGCGTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22810.12958335061300708No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAGCGTTCTGTAGGGGC22640.12861758254618502No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT22020.12509536959659867No Hit
ACCTAGGACGGTCAGTTTGGTCCCTTCGCCGAAGATGACATGATTAGTAG21820.12395917187092566No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21300.12100505778417585No Hit
GGCCTGGCCTCTGCTGGTACCAGTGTACACTTCGACTTCCAATGTTGTCTCCCCCACAGACAATGGTGGCCGT21280.12089143801160852No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA21200.12043695892133932No Hit
GGACAGACGGCCACCATTGTCTGTGGGGGAGACAACATTGGAAGTCGAAG20980.11918714142309901No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT20340.11555130870094538No Hit
CTGCACAGGGTCTGTGACTTCCTATGTGTTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAC20130.11435830108898871No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGGTGATCGTCTATGATGAT19780.11236995506906093No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19620.11146099688852253No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19560.11112013757082063No Hit
GTAATAGTCGGCCTCGTCCCCGGCCTCGACCCTGCTGATGATCAGGGAGG18580.10555276871502285No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18550.10538233905617192No Hit
GTATTGATACGTGCGACCCATTCCAGCCCCTTCCCTGGAGCTTGACGGAC18420.10464381053448446No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCACCATTGTCTGTGGGGGAGACAA18040.10248503485570573No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCTGGATGCCTCCGGGGAC17660.100326259176927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT200.0063266553.7674364
GTGGTAT4500.042.505971
GACAGGT2800.035.376461
TATCAAC40100.031.5904522
ATCAACG41450.030.5554963
TCAACGC41900.030.3062254
CAACGCA42600.029.9684945
AACGCAG42900.029.7589266
GTATCAA43450.029.636441
GCCCCCA42500.028.76142970
ACGCAGA44650.028.6690127
CGCAGAG45850.027.769788
GACAGGA3200.027.7522261
ACGGGAT1603.6379788E-1227.74197416
AGAGTAC46200.027.33619311
CAGAGTA46300.027.27792510
GCAGAGT47200.026.9024319
GGATACG658.254842E-426.75650448
AATGGTA6500.026.7124370
CTAATGG1308.3313294E-426.7124370
Produced by FastQC (version 0.11.5)