FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486050
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG74210.4993775444971569No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC66210.4455435550620774No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG60030.4039567982234783No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA59490.4003230039366105No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC52560.3536893105884728No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48600.3270414858181084No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG47640.3205814070858989No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46980.3161401029575048No Hit
GTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACC40610.2732747888698227No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG40400.27186164664715184No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC40080.2697082870697487No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC39440.2654015679149423No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT35710.24030147034083643No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG35400.23821540325022714No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35050.2358601662124424No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG34420.23162073954442985No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34380.23135156959725448No Hit
ATGCTACACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG34290.23074593721610984No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT34170.22993842737458361No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34090.22940008748023283No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA32940.22166145149894015No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31270.2104236062043673No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31010.2086740015477272No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG30040.20214663032872382No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC29710.1999259782645268No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28570.1922546347700279No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28250.19010127519262476No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28200.18976481275865548No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28160.1894956428114801No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27620.1858618485246122No Hit
GTAATACATAGCCGTGTCCTCGGCTCTGAGGCTGTTCATTTGCAGATACA26390.17758487264896874No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26020.1750950506375963No Hit
CCCCTGAGGGCCGCTTATTATTCTTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA25800.17361461592813163No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25360.17065374650920226No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTTTGGCACCTCAG25230.1697789441808822No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24930.16776016957706671No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24880.16742370714309748No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG24790.16681807476195284No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24740.16648161232798359No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGTAG24120.1623094781467649No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGC24110.16224218565997106No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTTTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT23870.16062716597691867No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23770.15995424110898018No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22750.15309040745600755No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21360.14373675179166245No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21090.1419198546482285No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20990.14124692978029005No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATCTCTTGTTCTGG20910.14070858988593923No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAAACTTGGAGCCAGAGAATCGCTC20640.1388916927425053No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC20220.13606540829716363No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19760.13296995390464653No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19730.132768076444265No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19620.13202785908953266No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19570.1316913966555634No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG19320.13000908448571716No Hit
TTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCG19050.12819218734228324No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19030.12805760236869554No Hit
CCCAACCACTCAGGCTGTCATCCCATGCTACACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGG18740.1261061202516739No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTGAGGCTGTTCATTTGCAGATA18450.12415463813465226No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18290.12307795834595069No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGAC17660.11883853167793815No Hit
GGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC17570.11823289929679351No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACAAAATAACTT17570.11823289929679351No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCCGAAGTTATTTTGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC17540.11803102183641197No Hit
ATGTATTACTGTGCGCTTCAGTGGGATATTGGAGTGCCTAGGGACTACTGGGGCCAGGGAACCCTGGTCACCGT17500.11776185188923657No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17390.11702163453450422No Hit
GCTGGTACCAGTATACAAAATAACTTCGGATGTTGGAGCTGCTTCCAGAA17300.11641600215335957No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17150.11540661485145184No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17090.11500285993068873No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16990.11432993506275024No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16580.11157094310420242No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16550.11136906564382087No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT16490.11096531072305776No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC16270.10948487601359308No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16140.10861007368527303No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC16000.10766797887015915No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15930.10719693146260219No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15880.10686046902863296No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15840.10659129908145756No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15780.10618754416069447No Hit
GCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGCTGTCATCCCATGCTACACAGTAATAATCAGCCTCATCCT15700.10564920426634367No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAAGAATAATAAGCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGG15570.1047744019380236No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAAACTTGGAGCCAGAGAATCGCTCAGGGACC15280.10282291982100199No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15160.10201540997947578No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC15140.10188082500588809No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14970.10073685273039265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG350.004411434858.89311670
AGCGTTT400.007482121251.53147570
CGTATGC1450.047.6167941
CAACTAG306.7878905E-445.32644719
GGTATCA13750.044.755591
GTGGTAT3250.042.8915671
ACGAGAC1650.041.21140722
ATCTCGT1250.041.17617837
TCTCGTA1350.040.72176738
GAACGAA350.001365722739.32358639
TCGTATG1500.039.06906540
TGGTATC3750.038.9859922
CGAGACT1750.038.8630123
ATGCCGT1750.037.6665544
GTATGCC1850.037.40178342
GTCTAGG750.00193086936.64460870
CCGTCTT1750.035.8317447
GACTAGG1900.035.821426
CACGAGA1900.035.78644621
CTTATAC2200.033.9994131
Produced by FastQC (version 0.11.5)