FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486050
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG69200.46566400861343826No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60080.40429326065744764No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC59030.3972275495440934No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50350.3388176710070321No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA45710.30759395713468596No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42890.28861747585882036No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40570.2730056189226473No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT39050.26277716092998216No Hit
GTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACC36310.24433901954846743No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCAC34830.2343797315029777No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC34520.23229366441236834No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33430.22495878335183875No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33140.22300730123481716No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG32750.22038289424985702No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32430.21822953467245382No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32270.21715285488375222No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC32150.21634534504222602No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31310.2106927761515427No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA30900.20793378419299485No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30840.20753002927223177No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30350.20423269741933311No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29990.20181016789475453No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28700.19312943709834796No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28290.19037044513980014No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27830.18727499074728307No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG27300.18370848894720904No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGG27270.1835066114868275No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27020.18182429931698124No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTTTGGCACCTCAG25290.1701826991016453No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24850.16722182968271593No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTTTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT24460.1645974226977558No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG24240.1631169879882911No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24130.16237677063355876No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23630.15901214629386629No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22830.15362874735035834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22650.15241748258806903No Hit
CCCCTGAGGGCCGCTTATTATTCTTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA22540.1516772652333367No Hit
GTAATACATAGCCGTGTCCTCGGCTCTGAGGCTGTTCATTTGCAGATACA22380.15060058544463512No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22310.15012953803707818No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGGGGGTCACCATCTCTTGTTCTGG22270.14986036808990275No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGTAG22220.14952390565593351No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCCGAAGTTATTTTGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC22030.14824534840685036No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21980.14790888597288113No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC21950.14770700851249957No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21630.1455536489350964No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21500.14467884660677635No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20420.1374112580330406No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19790.13317183136502808No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19660.13229702903670806No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19440.13081659432724338No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19320.13000908448571716No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18760.1262407052252616No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACAAAATAACTT18660.1255677803573231No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18260.12287608088556912No Hit
GCTGGTACCAGTATACAAAATAACTTCGGATGTTGGAGCTGCTTCCAGAA18260.12287608088556912No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAAACTTGGAGCCAGAGAATCGCTC18240.12274149591198141No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT18160.12220315601763063No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17970.1209245987685475No Hit
TTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCG17800.11978062649305204No Hit
GAATAATAAGCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAAGTTTGGCACCTCAGCCTCCCTGGCCAT17790.1197133340062582No Hit
GGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC17370.11688704956091653No Hit
ATGCTACACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG17260.11614683220618417No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGTAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGAC17230.11594495474580263No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGGC17130.11527202987786414No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAAGAATAATAAGCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGG17070.11486827495710103No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC16940.11399347262878101No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16890.11365701019481174No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC16520.1111671881834393No Hit
ATGTATTACTGTGCGCTTCAGTGGGATATTGGAGTGCCTAGGGACTACTGGGGCCAGGGAACCCTGGTCACCGT16470.11083072574947007No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16440.11062884828908852No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG16410.11042697082870698No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG15850.1066585915682514No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15770.1061202516739006No Hit
CAGTAATACATAGCCGTGTCCTCGGCTCTGAGGCTGTTCATTTGCAGATA15670.10544732680596211No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15510.10437064701726052No Hit
GTCTGGGACCCCCGGGCAGGGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCCGAAGTTATTTTGTATA15500.10430335453046667No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC15480.10416876955687898No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15200.10228457992665117No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC15180.10214999495306348No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG15180.10214999495306348No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGGGG15150.10194811749268194No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC15150.10194811749268194No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG14920.1004003902964234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGCG200.007831139550.94316520
GGTATCA12900.047.151121
GTGGTAT3350.042.602311
TGGTATC3300.042.196772
CGCTACT350.001431529838.9488930
GTATCAA31200.035.178441
TCTGGAG10850.032.0782470
TATCAAC34600.031.5092622
ATCAACG36000.030.3690433
CGCGCTA450.004935161730.25985328
TCAACGC36200.030.1044244
AACGCAG36450.029.8969426
CAACGCA36650.029.7337935
ACGCAGA38400.028.3787387
CGCAGAG38450.028.2535428
CAGAGTA38950.027.71373710
AGAGTAC38750.027.68157411
GCAGAGT39150.027.5749429
CCTAACG500.00835062827.17062
TACGTAC1254.940375E-927.15506212
Produced by FastQC (version 0.11.5)