FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999809
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38290.3829731478712434No Hit
ACATATTACTGCGCGAGGCGGACGAAACGTAAATCGGCTTCTGGACTTCT35080.3508670155999796No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33820.3382646085402312No Hit
GTAATATGTAGCCGTGTCTGCGGCAGTCACAGAATATAATTTCAGGGTGAACTGATTCTTCGACGTGTCTATGGA32050.3205612271943941No Hit
GTACGATACTGATTCTTGGACGTTCGGCCCAGGGACCGCGTTGGTGGTCAAACGAACTGTGGCTGCACCAT27600.27605272607067954No Hit
GTATCGTACTGCTGGCAGTAATAAGTCGCAAAGTCATCAGGCAGCAGGCCGTCGATGGTGAGAGTGAACTCTGTC25340.2534484086460514No Hit
GAGTTCACTCTCACCATCGACGGCCTGCTGCCTGATGACTTTGCGACTTA25160.25164806478037305No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24880.2488475298782067No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23230.23234437777615524No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22600.226043174246281No Hit
GCCCAGGGACCGCGTTGGTGGTCAAACGAACTGTGGCTGCACCATCTGTC22400.2240427921733051No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22200.22204241010032916No Hit
GTCCCTGGGCCGAACGTCCAAGAATCAGTATCGTACTGCTGGCAGTAATAAGTCGCAAAGTCATCAGGCAGCAGG21360.21364080539383024No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20440.2044390478581409No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20190.201938570266921No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCGTCACTTGCCG19620.19623748135893956No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19550.195537347633398No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19380.19383702287136842No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19180.1918366407983925No Hit
GAGTATTGGTTATTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCTCAACTCCTCATCTATGATGTCTC18500.1850353417502743No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17830.1783340618058049No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17790.17793398539120972No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17660.17663373704377536No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17510.1751334504890434No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17160.17163278186133551No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17080.17083262903214513No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17000.17003247620295475No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16660.16663182667889567No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16110.16113077597821185No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16070.16073069956361666No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15960.15963048942347988No Hit
GTCTATGGACGTGGCGATTCGGCCCCTGAGGGACGGATTGGAGTACGAGGCTCCAGTAAAGTAGACACTTCCAAG15830.15833024107604554No Hit
GTGGGGTCCCGTCTCGCTTCAGCGGCAGTGGATCTGGGACAGAGTTCACT15670.15672993541766478No Hit
TCCATAGACACGTCGAAGAATCAGTTCACCCTGAAATTATATTCTGTGACTGCCGCAGACACGGCTACATATTA15500.15502961065563522No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15400.15402941961914726No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15390.15392940051549847No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15210.15212905664982013No Hit
GTATTGGTTATTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCT14940.1494285408513026No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14820.14822831160751704No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14710.14712810146738026No Hit
TCTATGATGTCTCCCATTTGAACAGTGGGGTCCCGTCTCGCTTCAGCGGCAGTGGATCTGGGACAGAGTTCACT14520.14522773849805312No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14460.14462762387616035No Hit
ATAGGAGACAGAGTCACCGTCACTTGCCGGGCCAGCCAGAGTATTGGTTATTGGTTGGCCTGGTATCAGCAGAGA14420.14422754746156516No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14370.14372745194332118No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14040.1404268215229109No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14000.1400267451083157No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13920.13922659227912532No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13900.1390265540718277No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAAGCGAGACGGGACCCCA13800.13802636303533977No Hit
GGTATCAGCAGAGACCAGGAAAAGCCCCTCAACTCCTCATCTATGATGTCTCCCATTTGAACAGTGGGGTCCCGT13640.136426057376959No Hit
GTCTGCATCTATAGGAGACAGAGTCACCGTCACTTGCCGGGCCAGCCAGA13490.13492577082222706No Hit
GGCCTGGAGTGGCTTGGAAGTGTCTACTTTACTGGAGCCTCGTACTCCAA13380.13382556068209028No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTACGGATCCCTGG13380.13382556068209028No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13370.1337255415784415No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13260.13262533143830474No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13240.13242529323100713No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12930.12932470101789442No Hit
GTGCAGCCACAGTTCGTTTGACCACCAACGCGGTCCCTGGGCCGAACGTC12910.12912466281059684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCACCAACGCGGTCC12880.12882460549965044No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12860.12862456729235283No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12760.12762437625586487No Hit
GGACCCCACTGTTCAAATGGGAGACATCATAGATGAGGAGTTGAGGGGCTTTTCCTGGTCTCTGCTGATACCAGG12750.12752435715221608No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12710.1271242807376209No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12650.12652416611572812No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC12490.12492386045734735No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12370.1237236312135618No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12300.12302349748802022No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12100.12102311541504428No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12060.12062303900044909No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11990.11992290527490751No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11860.11862265692747313No Hit
GTTCAAATGGGAGACATCATAGATGAGGAGTTGAGGGGCTTTTCCTGGTC11820.11822258051287797No Hit
CTCCAGGCCCTTCCCGGGGGGCTGGCGGATCCAGGCCCAGTAGAAATTGT11810.11812256140922917No Hit
CAGTAATATGTAGCCGTGTCTGCGGCAGTCACAGAATATAATTTCAGGGT11710.11712237037274119No Hit
CATTTGAACAGTGGGGTCCCGTCTCGCTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCGACGGC11500.11502196919611646No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11460.11462189278152127No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11380.11382173995233089No Hit
GGACTGGTGAAGGCTTCGGAGACCCTGTCCCTCAATTGTCTTGTCTCTGGTGGGTCCATTAGCGATAACAATTT11140.11142128146475977No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCGTCACTT11090.11092118594651579No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10910.10912084208083744No Hit
ATCTGGGACAGAGTTCACTCTCACCATCGACGGCCTGCTGCCTGATGACTTTGCGACTTATTACTGCCAGCAGTA10860.10862074656259346No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTACGGATCCCTGG10650.10652034538596873No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10610.10612026897137354No Hit
GTCCCAGATGCAGCTGCAAGAGTCGGGCCCAGGACTGGTGAAGGCTTCGGAGACCCTGTCCCTCAATTGTCTTGT10550.10552015434948075No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10490.10492003972758797No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10430.10431992510569518No Hit
GATGGGTCCTGTCCCAGATGCAGCTGCAAGAGTCGGGCCCAGGACTGGTG10410.1041198868983976No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10330.10331973406920722No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10300.10301967675826083No Hit
GTCCATTAGCGATAACAATTTCTACTGGGCCTGGATCCGCCAGCCCCCCG10240.10241956213636805No Hit
GGTTATTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCTCAACT10190.10191946661812405No Hit
GTCATCAGGCAGCAGGCCGTCGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCGCTGAAGCGAGACGGGAC10130.10131935199623127No Hit
ATACTGATTCTTGGACGTTCGGCCCAGGGACCGCGTTGGTGGTCAAACGA10080.1008192564779873No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10040.10041918006339211No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10020.10021914185609451No Hit
GGTGAAGGCTTCGGAGACCCTGTCCCTCAATTGTCTTGTCTCTGGTGGGT10010.10011912275244572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT503.2812156E-452.5184370
GTGGTAT1600.042.5653151
GTATCAA18550.038.182581
TATCAAC20150.034.6385352
CGTATGC709.544747E-734.58688441
AACGGCC400.002632124334.4033437
TAACGGC400.002644542434.37033536
ATCAACG20900.033.395523
TCAACGC21000.033.0743684
CAACGCA21450.032.6979525
AACGCAG22000.031.8805076
AATGGTA3550.031.43708870
ACGCAGA23000.030.3418147
TCTATAC450.004930995430.2641243
CGCAGAG23150.030.1452148
AGAGTAC23400.029.82464211
TGGTACG500.005503934329.59102467
GATTCAC1401.6370905E-1129.1876451
TAGTCCT1052.6497219E-829.1832664
GCAGAGT24350.028.6596189
Produced by FastQC (version 0.11.5)