FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999809
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35920.35926862030647855No Hit
ACATATTACTGCGCGAGGCGGACGAAACGTAAATCGGCTTCTGGACTTCT34070.34076508613145107No Hit
GTAATATGTAGCCGTGTCTGCGGCAGTCACAGAATATAATTTCAGGGTGAACTGATTCTTCGACGTGTCTATGGA31100.3110594123477584No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28450.28455434988082723No Hit
GTACGATACTGATTCTTGGACGTTCGGCCCAGGGACCGCGTTGGTGGTCAAACGAACTGTGGCTGCACCAT27050.2705516753699957No Hit
GAGTTCACTCTCACCATCGACGGCCTGCTGCCTGATGACTTTGCGACTTA25440.25444859968253936No Hit
GTATCGTACTGCTGGCAGTAATAAGTCGCAAAGTCATCAGGCAGCAGGCCGTCGATGGTGAGAGTGAACTCTGTC25390.2539485041642954No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24080.24084600158630298No Hit
GCCCAGGGACCGCGTTGGTGGTCAAACGAACTGTGGCTGCACCATCTGTC21770.2177415886434309No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20460.20463908606543849No Hit
GTGGGGTCCCGTCTCGCTTCAGCGGCAGTGGATCTGGGACAGAGTTCACT20270.20273872309611135No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20170.20173853205962342No Hit
GTCCCTGGGCCGAACGTCCAAGAATCAGTATCGTACTGCTGGCAGTAATAAGTCGCAAAGTCATCAGGCAGCAGG20140.20143847474867702No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19950.19953811177934985No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT19710.1971376532917787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19650.19653753866988596No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19400.19403706107866603No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19130.1913365452801485No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18830.1883359721706846No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17750.17753390897661453No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCGTCACTTGCCG17640.17643369883647778No Hit
GAGTATTGGTTATTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCTCAACTCCTCATCTATGATGTCTC17490.17493341228174583No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17160.17163278186133551No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16710.16713192219713965No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16540.1654315974351101No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAAGCGAGACGGGACCCCA16320.16323117715483657No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16100.16103075687456306No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16010.16013058494172389No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15600.1560298016921232No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15580.1558297634848256No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15430.15432947693009366No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14910.1491284835403562No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14810.14812829250386825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14710.14712810146738026No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14510.14512771939440433No Hit
TCCATAGACACGTCGAAGAATCAGTTCACCCTGAAATTATATTCTGTGACTGCCGCAGACACGGCTACATATTA14430.14432756656521395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14360.1436274328396724No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14210.14212714628494041No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13930.1393266113827741No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13920.13922659227912532No Hit
GTATTGGTTATTGGTTGGCCTGGTATCAGCAGAGACCAGGAAAAGCCCCT13890.13892653496817892No Hit
ATAGGAGACAGAGTCACCGTCACTTGCCGGGCCAGCCAGAGTATTGGTTATTGGTTGGCCTGGTATCAGCAGAGA13850.13852645855358373No Hit
GTCTATGGACGTGGCGATTCGGCCCCTGAGGGACGGATTGGAGTACGAGGCTCCAGTAAAGTAGACACTTCCAAG13800.13802636303533977No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13710.13712619110250057No Hit
TCTATGATGTCTCCCATTTGAACAGTGGGGTCCCGTCTCGCTTCAGCGGCAGTGGATCTGGGACAGAGTTCACT13610.1361260000660126No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13590.135925961858715No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13360.13362552247479267No Hit
GTGCAGCCACAGTTCGTTTGACCACCAACGCGGTCCCTGGGCCGAACGTC13280.1328253696456023No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG13140.13142510219451917No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13110.13112504488357277No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12870.12872458639600165No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12840.12842452908505525No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12810.12812447177410885No Hit
GTCTGCATCTATAGGAGACAGAGTCACCGTCACTTGCCGGGCCAGCCAGA12720.12722429984126968No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTACGGATCCCTGG12640.12642414701207932No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12380.1238236503172106No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12360.123623612109913No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12230.12232336376247863No Hit
GGTATCAGCAGAGACCAGGAAAAGCCCCTCAACTCCTCATCTATGATGTCTCCCATTTGAACAGTGGGGTCCCGT12180.12182326824423464No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12120.12122315362234187No Hit
GTCCCAGATCCACTGCCGCTGAAGCGAGACGGGACCCCACTGTTCAAATG11900.11902273334206832No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11770.11772248499463397No Hit
GGACTGGTGAAGGCTTCGGAGACCCTGTCCCTCAATTGTCTTGTCTCTGGTGGGTCCATTAGCGATAACAATTT11600.11602216023260442No Hit
GTTCAAATGGGAGACATCATAGATGAGGAGTTGAGGGGCTTTTCCTGGTC11460.11462189278152127No Hit
CTCCAGGCCCTTCCCGGGGGGCTGGCGGATCCAGGCCCAGTAGAAATTGT11320.11322162533043811No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11320.11322162533043811No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11310.11312160622678931No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11310.11312160622678931No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC11300.1130215871231405No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11260.11262151070854534No Hit
GGACCCCACTGTTCAAATGGGAGACATCATAGATGAGGAGTTGAGGGGCTTTTCCTGGTCTCTGCTGATACCAGG11220.11222143429395015No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11200.11202139608665256No Hit
GTCCCAGATGCAGCTGCAAGAGTCGGGCCCAGGACTGGTGAAGGCTTCGGAGACCCTGTCCCTCAATTGTCTTGT11140.11142128146475977No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11050.1105211095319206No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACCACCAACGCGGTCC10940.10942089939178384No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCGTCACTT10900.10902082297718865No Hit
GGTGAAGGCTTCGGAGACCCTGTCCCTCAATTGTCTTGTCTCTGGTGGGT10870.10872076566624225No Hit
CAGTAATATGTAGCCGTGTCTGCGGCAGTCACAGAATATAATTTCAGGGT10840.10842070835529587No Hit
GGCCTGGAGTGGCTTGGAAGTGTCTACTTTACTGGAGCCTCGTACTCCAA10840.10842070835529587No Hit
GATGGGTCCTGTCCCAGATGCAGCTGCAAGAGTCGGGCCCAGGACTGGTG10730.10732049821515909No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10720.1072204791115103No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10710.10712046000786149No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10640.10642032628231993No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTACGGATCCCTGG10570.10572019255677835No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC10530.10532011614218316No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10490.10492003972758797No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10460.10461998241664157No Hit
ATCTGGGACAGAGTTCACTCTCACCATCGACGGCCTGCTGCCTGATGACTTTGCGACTTATTACTGCCAGCAGTA10440.10441994420934397No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10360.10361979138015362No Hit
CATTTGAACAGTGGGGTCCCGTCTCGCTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCGACGGC10300.10301967675826083No Hit
GACTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10280.10281963855096324No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10240.10241956213636805No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10140.10141937109988007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)