FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943445
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC38460.40765492424041677No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30710.3255091711758502No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGCCGGTTGTGTATGAG27770.2943467822713566No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26260.2783416097387765No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26240.27812962069860986No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25400.2692260810116117No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG25170.2667882070496955No Hit
TCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCTCATAC24130.2557647769610311No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23910.25343289751919823No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23290.24686123727403292No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTG22270.23604979622553512No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21970.2328699606230358No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21740.23043208666111964No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21520.22810020721928675No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21310.22587432229753723No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATT21290.22566233325737062No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG18840.19969367583695924No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18080.19163809231062753No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18020.1910021251901277No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACACCCGGGCAAAGC17910.18983618546921124No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17750.18814027314787826No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17570.18623237178637864No Hit
CTATTATTGCAGCTCATACACAACCGGCAGTACTCTTTGGGTGTTCGGCG17530.1858083937060454No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17510.1855964046658788No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17440.1848544430252956No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC16870.17881275538054683No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16590.17584490881821407No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTGTGTCTCCTGGTACCAACAACA15850.16800131433204904No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15720.16662338557096598No Hit
CCCAAAGAGTACTGCCGGTTGTGTATGAGCTGCAATAATAGTCAGCCTCG15640.1657754294102995No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGGCAGAGAAG14940.15835581300446766No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC14100.1494522733174695No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13920.1475443719559699No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13920.1475443719559699No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13870.14701439935555333No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13670.14489450895388709No Hit
CTCTTTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC13270.14065472815055463No Hit
GCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGAGATGGTCAGGG13110.13895881582922162No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13100.13885282130913834No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC12810.13577898022672227No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT12720.13482502954597247No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12680.13440105146563922No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12600.13355309530497272No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12590.13344710078488942No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12420.13164519394347313No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12280.13016127066230676No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11720.12422557753764131No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC11500.12189369809580844No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11330.12009179125439214No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11270.11945582413389229No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC11020.11680596113180948No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10930.11585201045105967No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10510.11140024060756058No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAAGAGTACTGC10490.11118825156739398No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT10150.10758443788456136No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC10100.1070544652841448No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10060.10663048720381156No Hit
GTGTATGAGCTGCAATAATAGTCAGCCTCGTCCTCAACTTGCAGCCCAGA10000.10599452008331169No Hit
GACCATCTCTGGGCTGCAAGTTGAGGACGAGGCTGACTATTATTGCAGCT9930.10525255844272852No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9830.1041926132418954No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9810.10398062420172877No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9770.10355664612139552No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9670.1024967009205624No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9560.10133076119964597No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9530.10101277763939603No Hit
CTCCTGATTTATGAGGTCGATAATCGGCAGTCAGGAGTTTCTGATCGCTT9500.1006947940791461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCG200.007805734450.98359329
GGTATCA13000.046.3956071
CCTAACG504.3639684E-640.639752
CTAACGC558.425855E-636.925643
GTATCAA30650.034.6030271
GTGGTAT2950.034.4587441
TGCACCG1900.032.0602076
CGACCAA450.004291333731.13576349
TTAACGG553.437211E-431.07616635
TATCAAC34500.030.4307332
TGGTATC3450.030.430732
ATCAACG35250.029.7674773
CGACCGG1950.029.49956711
TCAACGC35400.029.3482694
AGCGACC605.066521E-429.08446147
CGGTACC605.16787E-428.9862245
CAACGCA35900.028.939525
AACGGCC605.6448724E-428.5522237
CCGACCG2150.028.32927110
AACGCAG36950.028.3003276
Produced by FastQC (version 0.11.5)