FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711384
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACCTGTGGGGGAAACAATATTGGGAGTAAAAGTGTACACTGGTACCAGCAAAAGCCAGGCCAGGCCC60340.8482057510430373No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50050.7035581345658604No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAT44200.6213240668893313No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28260.39725380385277154No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26990.37940127975889254No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC25780.36239218200015744No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23660.3325911181584067No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCGAGTCTCC23120.3250002811421117No Hit
CCCCAGGACAGACGGCCAGTATTACCTGTGGGGGAAACAATATTGGGAGT20830.29280950935078665No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19110.2686312877433285No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18910.2658198666261822No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18670.26244616128560666No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18230.2562610348278848No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17080.2400953634042936No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCTTCCTCTGACT17040.23953307918086436No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16800.2361593738402888No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16760.23559708961685952No Hit
CCCTTCCTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG16640.23391023694657176No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16500.23194224216456935No Hit
CACTACTACTATCCCAAAGTTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG16330.229552534214995No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16070.2258976867627048No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15850.22280512353384388No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15840.22266455247798655No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15540.21844742080226712No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15260.21451143123826233No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGC15240.21423028912654768No Hit
GGCCAGGCCCCTGTGGCGGTCGTCTATGATGATAGCGACCGGCTCTCAGG14980.21057544167425749No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14810.20818573372468316No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14780.2077640205571112No Hit
GGCTCTGTGACCTCCCTTCCTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGTATTAC14720.20692059422196732No Hit
GCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGCCACCCTCGGTGTCA14390.20228174937867593No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT14230.20003261248495888No Hit
CAGGTAATACTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGAGGAAGGGAGGTCACAGAG14170.199189186149815No Hit
GCTCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACGG13940.19595605186509676No Hit
GTAGTAGTGATTATATGATATTCGGCGGAGGGACCAAGCTGACCGTCCTG13920.19567490975338214No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGCCAC13890.1952531965858102No Hit
GAGTAAAAGTGTACACTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGG13700.1925823465245212No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG13510.18991149646323224No Hit
CCCCTGTGGCGGTCGTCTATGATGATAGCGACCGGCTCTCAGGGATCCCT13400.18836521484880175No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13340.1875217885136579No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCATATAATCACTA13290.1868189332343713No Hit
GGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGAGCCGGTCGCTATCATCATAGA13100.18414808317308232No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12460.1751515355982142No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG12440.17487039348649958No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCT12350.17360525398378374No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCATATAATCACTAC12260.1723401144810679No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11570.16264071162691318No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11560.16250014057105586No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11480.16137557212419734No Hit
AGTGTACACTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGGCGGTCGT11360.15968871945390958No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCGAGT11340.15940757734219493No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG11280.15856415100705104No Hit
CCCCTGGGATCCTGCAGCTCGAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11000.15462816144304622No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT10850.15251959560518652No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC10820.15209788243761455No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10770.151395027158328No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10750.15111388504661338No Hit
CTCACTGCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG10490.1474590375943232No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10120.14225790852760253No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9940.13972762952217085No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGTATTACCTGTGGGGGAAACAATATTGGGAGTAAAAGTGTA9890.13902477424288429No Hit
CTATTACTGTCAACTTTGGGATAGTAGTAGTGATTATATGATATTCGGCG9810.13790020579602577No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9780.13747849262845382No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9720.13663506629330993No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT9700.1363539241815953No Hit
GACTATTACTGTCAACTTTGGGATAGTAGTAGTGATTATATGATATTCGG9540.13410478728787828No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCATATAATCA9480.1332613609527344No Hit
GCTTGGTCCCTCCGCCGAATATCATATAATCACTACTACTATCCCAAAGTTGACAGTAATAGTCGGCCTCATCCC9430.13255850567344782No Hit
CCACAGGGGCCTGGCCTGGCTTTTGCTGGTACCAGTGTACACTTTTACTC9420.1324179346175905No Hit
GTGTACACTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGGCGGTCGTC9130.12834137399772835No Hit
CTACTACTATCCCAAAGTTGACAGTAATAGTCGGCCTCATCCCCGGCTTC9080.1276385187184418No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9050.12721680555086984No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC8930.12552995288058208No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8670.1218751054282919No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCC8500.11948539747871756No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8460.11892311325528827No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8420.11836082903185902No Hit
CTGCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGCCACCCTCGGTGT8410.11822025797600172No Hit
GTGTACACTTTTACTCCCAATATTGTTTCCCCCACAGGTAATACTGGCCG8370.11765797375257246No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8210.11540883685885542No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8190.11512769474714078No Hit
TCTATGATGATAGCGACCGGCTCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACGGCCACC8120.11414369735613958No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7990.11231627362999448No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7970.11203513151827986No Hit
GGCCTGGCCTGGCTTTTGCTGGTACCAGTGTACACTTTTACTCCCAATATTGTTTCCCCCACAGGTAATACTGGC7940.11161341835070791No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7800.10964542356870552No Hit
CAATATTGGGAGTAAAAGTGTACACTGGTACCAGCAAAAGCCAGGCCAGG7790.1095048525128482No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACGGCCACC7440.10458486555784216No Hit
GTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATG7430.10444429450198486No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7420.10430372344612754No Hit
GGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7400.10402258133441292No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7360.10346029711098366No Hit
CTTTTACTCCCAATATTGTTTCCCCCACAGGTAATACTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGG7360.10346029711098366No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT7350.10331972605512635No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC7350.10331972605512635No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7240.10177344444069589No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC7230.10163287338483856No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCGAGTCTCCCGTGGTGGGG7220.10149230232898127No Hit
CCCCTCCCCTGGGATCCTGCAGCTCGAGTCTCCCGTGGTGGGGGGTGAGG7220.10149230232898127No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT7120.1000865917704081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT150.002366968.91360537
CTAAACG200.003176099463.9845369
CGAGCTA200.007103187552.21306249
CGCGTAT200.0073531551.75839239
GCATCGC200.00742545851.6304635
GGTATCA5450.046.532081
GTGGTAT1250.041.12431
CCTATAC504.0251107E-641.1185153
GTATCAA16600.040.463671
TAGCAAG350.001296529339.73880848
ATCTCCG955.456968E-1239.67297410
CCCCTAT451.0413544E-438.078061
CTATACC451.04221705E-438.07274
ACGAGAC1009.094947E-1237.68667622
TGGTATC1450.035.4472
ATCAACG18850.035.2652173
AATGGTA3200.034.862970
TCAACGC19150.034.712764
TATCAAC19400.034.6186832
CAACGCA19050.034.5352365
Produced by FastQC (version 0.11.5)