FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711384
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACCTGTGGGGGAAACAATATTGGGAGTAAAAGTGTACACTGGTACCAGCAAAAGCCAGGCCAGGCCC60210.8463783273168922No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46320.6511251307310819No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAT40240.5656579287698347No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30520.42902286247652466No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26320.3699830190164524No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC23100.3247191390303971No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22380.3145980230086704No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCGAGTCTCC22150.3113648887239522No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22060.31009974922123634No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19650.2762221247596235No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19340.2718644220280468No Hit
CCCCAGGACAGACGGCCAGTATTACCTGTGGGGGAAACAATATTGGGAGT18610.26160273495046277No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18150.2551364663810263No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17630.2478267714764459No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGC17510.24613991880615815No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCTTCCTCTGACT17480.24571820563858618No Hit
CCCTTCCTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG16860.23700280017543268No Hit
GGGTGGCCGTGTTCCCAGACTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGAGCCGGTCGCTATCATCATAGA16600.23334795272314252No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15890.22336740775727315No Hit
GGCTCTGTGACCTCCCTTCCTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGTATTAC15770.22168055508698536No Hit
CACTACTACTATCCCAAAGTTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG15700.22069655769598417No Hit
GTAGTAGTGATTATATGATATTCGGCGGAGGGACCAAGCTGACCGTCCTG15690.22055598664012685No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15530.2183068497464098No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15460.2173228523554086No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15440.217041710243694No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA15420.21676056813197936No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15060.211700010121116No Hit
GGCCAGGCCCCTGTGGCGGTCGTCTATGATGATAGCGACCGGCTCTCAGG14950.21015372850668557No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14490.20368745993724907No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14480.20354688888139177No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14450.2031251757138198No Hit
GCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGCCACCCTCGGTGTCA13640.19173892018937733No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGCCAC13630.19159834913352003No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13440.18892749907223103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13400.18836521484880175No Hit
GAGTAAAAGTGTACACTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGG13390.18822464379294446No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13270.18653779112265667No Hit
CAGGTAATACTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGAGGAAGGGAGGTCACAGAG13220.1858349358433701No Hit
GCTCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACGG13050.18344522789379575No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG12940.18189894627936531No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCCT12690.17838466988293242No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT12440.17487039348649958No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG12350.17360525398378374No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12160.17093440392249476No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCGAGT12060.1695286933639216No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCATATAATCACTA11920.1675606985819192No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11890.16713898541434724No Hit
CCCCTGTGGCGGTCGTCTATGATGATAGCGACCGGCTCTCAGGGATCCCT11780.16559270379991678No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11720.1647492774647729No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT11650.1637652800737717No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG11530.1620784274034839No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCATATAATCACTAC11280.15856415100705104No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11120.156315014113334No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCC11020.15490930355476085No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10860.15266016666104384No Hit
AGTGTACACTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGGCGGTCGT10590.14886474815289633No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10400.14619389809160735No Hit
CCCCTGGGATCCTGCAGCTCGAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10150.14267962169517448No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10090.1418361953600306No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10000.14057105585731475No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9840.13832191896359772No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCATATAATCA9750.13705677946088188No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9740.13691620840502455No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC9660.13579163995816607No Hit
GCTTGGTCCCTCCGCCGAATATCATATAATCACTACTACTATCCCAAAGTTGACAGTAATAGTCGGCCTCATCCC9540.13410478728787828No Hit
CTATTACTGTCAACTTTGGGATAGTAGTAGTGATTATATGATATTCGGCG9540.13410478728787828No Hit
GTGTACACTGGTACCAGCAAAAGCCAGGCCAGGCCCCTGTGGCGGTCGTC9360.1315745082824466No Hit
CTCACTGCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG9300.13073108194730274No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9270.1303093687797308No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9250.13002822666801614No Hit
GACTATTACTGTCAACTTTGGGATAGTAGTAGTGATTATATGATATTCGG8950.1258110949922967No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8740.12285910281929309No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGTATTACCTGTGGGGGAAACAATATTGGGAGTAAAAGTGTA8530.11990711064628948No Hit
CTACTACTATCCCAAAGTTGACAGTAATAGTCGGCCTCATCCCCGGCTTC8480.11920425536700291No Hit
CTGCACAGGCTCTGTGACCTCCCTTCCTCTGACTCAGCCACCCTCGGTGT8460.11892311325528827No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT8410.11822025797600172No Hit
GTGTACACTTTTACTCCCAATATTGTTTCCCCCACAGGTAATACTGGCCG8360.11751740269671514No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8320.11695511847328588No Hit
GGCCTGGCCTGGCTTTTGCTGGTACCAGTGTACACTTTTACTCCCAATATTGTTTCCCCCACAGGTAATACTGGC8250.11597112108228468No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8040.11301912890928106No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7930.1114728472948506No Hit
CCACAGGGGCCTGGCCTGGCTTTTGCTGGTACCAGTGTACACTTTTACTC7850.11034827884799209No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAT7820.10992656568042014No Hit
GTGTCAGTGGCCCCAGGACAGACGGCCAGTATTACCTGTGGGGGAAACAA7700.10823971301013235No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC7670.10781799984256042No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7580.10655286033984458No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7540.10599057611641532No Hit
GTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATG7540.10599057611641532No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7530.105850005060558No Hit
GGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG7490.10528772083712874No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC7490.10528772083712874No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7470.10500657872541412No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT7360.10346029711098366No Hit
TCTATGATGATAGCGACCGGCTCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAACACGGCCACC7350.10331972605512635No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7290.10247629971998244No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCGAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC7280.10233572866412514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTAG300.002849545365.75577570
TGGTACG200.00577620655.01523667
ATAGACC200.00758793251.3485833
GGTATCA7550.048.101951
GTACATA306.529424E-445.6817251
GTGGTAT1850.038.8911971
GAATACG1150.038.6920439
ATACGTA1150.038.68932311
TACGTAC1150.038.68932312
TGGTATC1900.037.841122
CTAGAGA1200.037.0798764
AGAATAC1200.037.0798768
ACGTACC1200.037.0772713
GTATCAA18600.036.65591
CGTACCA1250.035.59417714
AATACGT1250.035.59417710
TATCAAC20800.033.084992
ATGGCAA2204.9112714E-1132.87788870
ATCAACG21100.032.772243
TCAACGC21200.032.451624
Produced by FastQC (version 0.11.5)