FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345826
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC64900.482231729807568No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA48280.3587387968429797No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37720.2802739730098839No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37700.2801253653889879No Hit
CTATAACTATGTCTCCTGGTACCAACAGCACCCACACAAAGCCCCCAAAG37360.2775990358337556No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35660.26496738805759434No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32310.2400756115575119No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG30940.22989598952613488No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30750.2284842171276227No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCAGCGGAACCAA30750.2284842171276227No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30030.2231343427753662No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28270.21005687213651691No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27860.2070104159081486No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27680.20567294732008445No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCC27290.20277509871261218No Hit
GATTATTACTGCAGCTCATATGCAGCCAGTGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAG26720.19853978151707577No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTC26080.19378433764840328No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25670.19073788142003498No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25350.18836015948569873No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA25290.1879143366230107No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT24800.18427344991105835No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24580.18263876608120216No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24140.17936939842148986No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCAGCGGAACCAAAGATAATATT23890.17751180316028967No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23290.17305357453340922No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22400.16644053540353657No Hit
TAGTTATAGTCACCAATATTATCTTTGGTTCCGCTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACA21990.16339407917516827No Hit
GTCAGTCACCATCTCCTGCAGCGGAACCAAAGATAATATTGGTGACTATAACTATGTCTCCTGGTACCAACAGCA21410.15908445816918382No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC21250.15789559720201574No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG21130.15700395147663962No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21090.1567067362348476No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19650.14600698753033453No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC19360.14385217702734232No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG19220.1428119236810702No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18890.14035989793628598No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18840.13998837888404592No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18220.13538154263626948No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18110.1345642007213414No Hit
CCCAAGCACTGGCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCA17770.13203787116610916No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17500.13003166828401294No Hit
GACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGCACTGGCTGCATATGAGCTGCAGTAATAATCAG17260.12824837683326076No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17130.12728242729743666No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16830.12505331298399644No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16790.1247560977422044No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC16660.12379014820638032No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16570.12312141391234825No Hit
CCCAAGCACTGGCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGG16470.12237837580786819No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAACTTCAGGGAAAACTGATTCTTGGACATGTCTAGGGA16070.11940622338994789No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGCCAGTGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGT16020.11903470433770784No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCATGACTTTGGGGGCTTTGTGTGGGTGCTGTTGGTACCAGGAGA15840.1176972357496437No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG15760.11710280526605965No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15330.11390774141679534No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACAC15120.11234736139738719No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG14920.11086128518842703No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG14770.10974672803170692No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14680.10907799373767485No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14480.1075919175287147No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCAGCGGAACCAAAGATAATATTGGTGA14450.1073690060973707No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14450.1073690060973707No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT14420.10714609466602666No Hit
ACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGCACTGGCTGCATATGAGCTGCAGTAATAATCAGC14390.10692318323468264No Hit
CCTTGGTCCCTCCGCCGAACACCCAAGCACTGGCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCAGC14360.10670027180333863No Hit
AGATAATATTGGTGACTATAACTATGTCTCCTGGTACCAACAGCACCCAC13980.10387672700631434No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13890.10320799271228227No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13820.10268786603914622No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT13800.1025392584182502No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13760.10224204317645817No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13740.10209343555556216No Hit
GTACCAACAGCACCCACACAAAGCCCCCAAAGTCATGATTTATGATGTCA13730.10201913174511415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13680.1016476126928741No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACA200.006985450652.4367949
ACGTAGA1900.046.62859327
TCGTATG1050.045.9890840
GGTATCA14000.044.727371
CGTAGAG2050.043.24410228
CGTATGC1300.042.52512741
AGACGTA2100.042.16267825
CGAGACG2150.041.16990723
ACGAGAC2250.040.84258722
CCCACGA2200.040.2177819
TCTCGTA1300.039.68167538
CTTATAC2300.038.470611
CACGAGA2500.035.39559621
GTATCAA38350.035.140941
ATCTCCG2450.034.7249310
ATCTCGT1600.034.3495137
CCGTCAT1201.5751884E-533.70109670
GACGTAG2700.032.80173526
TATACAC2950.032.301273
TACGTAT1052.8432574E-432.09628370
Produced by FastQC (version 0.11.5)