FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345826
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC62520.46454742292094225No Hit
GCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA43660.32441043641600026No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39530.29372296270097326No Hit
CTATAACTATGTCTCCTGGTACCAACAGCACCCACACAAAGCCCCCAAAG34180.2539704241112893No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33830.25136979074560906No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32360.24044713060975192No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32230.23948118107392785No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29030.2157039617305655No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCAGCGGAACCAA28710.21332623979622925No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27980.20790206163352468No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT27650.20545003588874045No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27250.20247788347082016No Hit
GTGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAGCCC26850.19950573105289984No Hit
GATTATTACTGCAGCTCATATGCAGCCAGTGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTGGGTCAG25380.18858307091704277No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25300.18798864043345873No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24950.18538800706777844No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATAT24290.18048395557820995No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23840.17714028410804963No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23160.17208762499758512No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23000.170898764030417No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTC22730.1688925611483208No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCAGCGGAACCAAAGATAATATT22060.16391420584830432No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21140.15707825528708763No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21010.15611230575126353No Hit
TAGTTATAGTCACCAATATTATCTTTGGTTCCGCTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACA20440.1518769885557271No Hit
GTCAGTCACCATCTCCTGCAGCGGAACCAAAGATAATATTGGTGACTATAACTATGTCTCCTGGTACCAACAGCA19980.14845901327511876No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19810.14719584849750264No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19590.1455611646676465No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG19480.1447438227527184No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19440.1444466075109264No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC19050.14154875890345409No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC18910.14050850555718197No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18820.13983977126314992No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18480.13731344170791765No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG18070.13426698547954935No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18030.13396977023775733No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17720.1316663521138691No Hit
CCCAAGCACTGGCTGCATATGAGCTGCAGTAATAATCAGCCTCATCCTCA17700.1315177444929731No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17030.1265393891929566No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACAC17010.12639078157206057No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16800.12483040155265243No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC16770.1246074901213084No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16610.12341862915414027No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15430.1146507795212754No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15260.11338761474365928No Hit
CCCCTGAGGGCCGCTTAGTGACATCATAAATCATGACTTTGGGGGCTTTGTGTGGGTGCTGTTGGTACCAGGAGA15110.11227305758693919No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14990.11138141186156308No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14780.10982103184215494No Hit
GACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGCACTGGCTGCATATGAGCTGCAGTAATAATCAG14740.1095238166003629No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAACTTCAGGGAAAACTGATTCTTGGACATGTCTAGGGA14720.10937520897946688No Hit
GGATGAGGCTGATTATTACTGCAGCTCATATGCAGCCAGTGCTTGGGTGTTCGGCGGAGGGACCAAGGTGACCGT14650.10885508230633083No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14640.10878077849588283No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG14580.10833495563319478No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14420.10714609466602666No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14100.10476837273169042No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14000.10402533462721035No Hit
ACCCAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGCACTGGCTGCATATGAGCTGCAGTAATAATCAGC13860.10298508128093824No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA13780.1023906507973542No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCAGCGGAACCAAAGATAATATTGGTGA13600.10105318220929005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)