FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978178
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGCACCGCATATGGAGGCAGCAACACCTTTTATGTCTTCGG48720.4980688586330913No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47130.48181414834518865No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA47030.4807918395220502No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG43310.44276195130129686No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39980.4087190674907839No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39530.4041186777866605No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG37720.38561488808785316No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37240.3807078057367882No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35210.35995493662707606No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC31730.3243785895818552No Hit
GACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATAAAAGGTGTTG30660.31343988517427296No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAATGACATT30510.3119064219395652No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATA29650.30311456606057385No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28740.2938115557700132No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC28140.28767770283118205No Hit
CTCCTGCACTGGAACCAGCAATGACATTGGTGGTAATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC28040.2866553940080435No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27400.280112617539957No Hit
ACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATAAAAGGTGTTGC26650.272445301366418No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26570.27162745430790713No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTC25910.26488021607519285No Hit
CCTTTTATGTCTTCGGAACTGGGACCGAGGTCACCGTCCTAGGTCAGCCC25660.2623244440173465No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25150.25711066901934004No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24850.25404374254992446No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAATGACATTGGTGGTAATAACTATGTCTCCTGGTACCAACAACA23550.24075372784912358No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23400.23922026461441576No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCACCGCATAT22270.2276681749129504No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21840.2232722469734547No Hit
GTGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG21700.2218410146210608No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21480.21959193521015602No Hit
TTACTGCACCGCATATGGAGGCAGCAACACCTTTTATGTCTTCGGAACTG21450.21928524256321444No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20260.20711976756786596No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19800.20241714698142874No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19380.1981234499242469No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19050.19474983080788977No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18920.19342082933780969No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT18070.1847312043411322No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17520.17910850581387028No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA17320.17706388816759322No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT17140.17522373228594387No Hit
CATAAAAGGTGTTGCTGCCTCCATATGCGGTGCAGTAATAATCAGCCTCA16750.17123672787570363No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA16520.168885417582485No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16270.16632964552463866No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC15720.16070694699737675No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15590.15937794552729667No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15570.15917348376266896No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG15400.15743555876333348No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15370.1571288661163919No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15230.15569763376399795No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC15220.1555954028816841No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15210.15549317199937024No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15090.15426640141160403No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14970.15303963082383779No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGC14760.15089278229524689No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTC14580.14905262641359754No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14070.14383885141559102No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13730.14036300141692No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13620.13923846171146764No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC13560.13862507641758454No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13430.13729607494750445No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC13090.13382022494883344No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12850.13136668377330096No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12820.1310599911263594No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12810.13095776024404554No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12650.1293220661270239No Hit
GAACTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACT12360.12635737053992216No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCACCGCATATGGAGGCAGCAACAC12350.12625513965760835No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12250.1252328308344698No Hit
CTCCATATGCGGTGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGC12180.12451721465827283No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12180.12451721465827283No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12170.12441498377595897No Hit
CATATGGAGGCAGCAACACCTTTTATGTCTTCGGAACTGGGACCGAGGTC12150.12421052201133126No Hit
GCATATGGAGGCAGCAACACCTTTTATGTCTTCGGAACTGGGACCGAGGT12070.12339267495282044No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11820.12083690289497412No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11580.11838336171944165No Hit
GTCACCATCTCCTGCACTGGAACCAGCAATGACATTGGTGGTAATAACTA11580.11838336171944165No Hit
GGCAAAGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTCCGG11380.1163387440731646No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11340.1159298205439092No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11310.11562312789696763No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTCCGGGGTCCCTGATCG11300.11552089701465378No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATTA11080.11327181760374902No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG11070.11316958672143516No Hit
CGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC11050.11296512495680745No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11010.11255620142755203No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10900.11143166172209965No Hit
CTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10720.10959150584045031No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC10590.10826250437037022No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT10570.1080580426057425No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA10480.10713796466491783No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10430.10662681025334858No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT10430.10662681025334858No Hit
CGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCGGAGGGCCGCTTAGTGACGT10390.10621788672409316No Hit
ATCAGGGACCCCGGAGGGCCGCTTAGTGACGTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTA10250.10478665437169922No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG10240.10468442348938536No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10150.10376434554856069No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA10130.10355988378393298No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA9940.10161749701996978No Hit
CCCCGGAGGGCCGCTTAGTGACGTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA9800.10018626466757584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2000.082.4785570
TAGACCG200.00760144651.3269235
GGTATCA6200.050.7802121
TCTCGTA2550.047.3215438
GATCTCG2750.043.7964836
TCGTATG2700.043.52777540
ATCTCGT2800.043.06090537
TGATCTC2800.042.98787335
ATGATCT2850.042.20116434
CGTATGC2800.042.0253241
GACTAGG2800.041.5418726
AGACTAG2800.041.53762425
AATGGTA3350.041.36237770
GTATCAA17900.041.1039771
GAGACTA2850.040.8047224
CGAGACT2850.040.8047223
ATGCCGT2950.040.00638244
ACGAGAC3000.039.90461322
CTAGGCA3050.039.2906128
CTTATAC3150.039.1102261
Produced by FastQC (version 0.11.5)