FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978178
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGCACCGCATATGGAGGCAGCAACACCTTTTATGTCTTCGG47020.4806896086397363No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC46660.4770092968764376No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43560.44531772335914327No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG42940.4389794086556843No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA41910.42844962777735746No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC36440.37252933515167996No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG36110.3691557160353228No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33870.34625599839701976No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33240.33981545281124703No Hit
GTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG32400.3312280586968834No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC31180.31875589105459334No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29520.30178556459049377No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATA28900.29544724988703486No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAATGACATT28670.29309593959381625No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28420.29054016753596995No Hit
CTCCTGCACTGGAACCAGCAATGACATTGGTGGTAATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC27730.2834862366563141No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26280.2686627587208054No Hit
GACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATAAAAGGTGTTG26050.2663114484275868No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25710.2628355984289158No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTC25350.2591552866656171No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG24740.25291920284447206No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23980.24514965578861925No Hit
ACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATAAAAGGTGTTGC23790.24320726902465603No Hit
CCTTTTATGTCTTCGGAACTGGGACCGAGGTCACCGTCCTAGGTCAGCCC23760.2429005763777145No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAATGACATTGGTGGTAATAACTATGTCTCCTGGTACCAACAACA22860.23369979696946774No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT22740.23247302638170148No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22010.22501017197279022No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20460.20916438521414302No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCACCGCATAT20290.20742646021480754No Hit
GTGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG19790.2023149160991149No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG19550.1998613749235824No Hit
TTACTGCACCGCATATGGAGGCAGCAACACCTTTTATGTCTTCGGAACTG19440.19873683521813004No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19270.19699891021879454No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17960.18360666463567982No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT17500.17890404404924257No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT17500.17890404404924257No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17380.17767727346147633No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT17170.17553042493288543No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16750.17123672787570363No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16190.16551179846612785No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA16160.1652051058191863No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16140.16500064405455858No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA16130.1648984131722447No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15850.16203594846745686No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15720.16070694699737675No Hit
CATAAAAGGTGTTGCTGCCTCCATATGCGGTGCAGTAATAATCAGCCTCA15440.15784448229258888No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15410.1575377896456473No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15290.15631101905788108No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15200.1553909411170564No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC14810.15140393670681615No Hit
CTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTC14680.15007493523673607No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGC14410.147314701414262No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14400.14721247053194816No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC14350.1467013161203789No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13900.14210092641625552No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13820.1412830793577447No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13760.1406696940638616No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13520.1382161528883291No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG13430.13729607494750445No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12780.13065106759710401No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT12540.12819752642157153No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12440.127175217598433No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12350.12625513965760835No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12220.12492613818752825No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12110.12380159848207586No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAAGATGAGGCTGATTATTACTGCACCGCATATGGAGGCAGCAACAC12070.12339267495282044No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA11780.12042797936571872No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11660.11920120877795248No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG11610.11869005436638322No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11570.1182811308371278No Hit
CTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC11520.11776997642555855No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTCCGGGGTCCCTGATCG11260.11511197348539835No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11260.11511197348539835No Hit
CGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC11240.11490751172077066No Hit
GAACTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACT11110.11357851025069057No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC11100.11347627936837673No Hit
GTCACCATCTCCTGCACTGGAACCAGCAATGACATTGGTGGTAATAACTA11030.11276066319217974No Hit
CTCCATATGCGGTGCAGTAATAATCAGCCTCATCTTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGC10980.11224950878061049No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10940.11184058525135507No Hit
GCATATGGAGGCAGCAACACCTTTTATGTCTTCGGAACTGGGACCGAGGT10900.11143166172209965No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT10780.11020489113433342No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA10680.10918258231119489No Hit
GGCAAAGCCCCCAAACTCATGATTTATGACGTCACTAAGCGGCCCTCCGG10630.10867142789962564No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10630.10867142789962564No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGG10600.10836473525268407No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10530.10764911907648711No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10380.1061156558417793No Hit
CATATGGAGGCAGCAACACCTTTTATGTCTTCGGAACTGGGACCGAGGTC10300.10529780878326848No Hit
GGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGTTCCGAA10140.10366211466624684No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGACATAGTTATTA10070.10294649849004987No Hit
CTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCC10000.10223088231385288No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG9970.10192418966691134No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9850.10069741907914512No Hit
GTCCCAGTTCCGAAGACATAAAAGGTGTTGCTGCCTCCATATGCGGTGCAGTAATAATCAGCCTCATCTTCAGC9830.1004929573145174No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT9830.1004929573145174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGT100.009443502131.7402870
CTAGGCT150.002439944368.3893621
TTCGGCT200.007621919351.2920216
ACAGTCG200.00762654351.284158
GTACGGC200.00762808451.2815314
GTGGTAT2350.048.040161
ATAGTGT306.598547E-445.585916
GGTATCA8100.045.1915441
TGGTATC2500.045.150832
CGAGTGA700.001692254237.6400870
GTATCAA18700.035.3080831
AGGATGG4950.033.39780469
TATCAAC21500.030.5460132
ATCAACG22200.029.262723
TCAACGC22700.028.6167074
AAGGATG5200.028.55103768
GACAGCT1202.9376679E-928.5086821
TCGTAAA500.00733778427.89923349
GTAAAAC1353.4015102E-1027.859483
GGTCGTA500.007407150727.84550947
Produced by FastQC (version 0.11.5)