FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414189
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74230.5248944801578855No Hit
GTTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA71380.5047415868741731No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66410.46959776946362897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56090.39662308220471243No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG48240.3411142357916799No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45990.32520405688348586No Hit
GTTTATTACTGTCAGCAATTTTATAGTACTCCTGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA44990.3181328662576218No Hit
CTCCTAAGCTTCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCT44840.31707218766374223No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43170.30526329931854934No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42120.29783854916139213No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA41790.295505056254857No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40600.2870903394100789No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGTATAAAACACT38270.2706144652518157No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37810.26736171756391824No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37010.26170476506322704No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36850.2605733745630888No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGACGGTGACCCTCTCGCCCA36690.2594419840629506No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35700.2524415053433452No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC34910.24685526474891262No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34770.24586529806129168No Hit
ATTTTATAGTACTCCTGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT34120.24126902415448007No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33640.23787485265406536No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG33360.23589491927882342No Hit
AACCAGGACAGCCTCCTAAGCTTCTCATTTACTGGGCATCTACCCGGGGA32400.22910657627799397No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31870.22535884524628605No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATTTTATAGTACT30470.2154591783700764No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29680.20987293777564386No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCGTCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAA29220.2066201900877464No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28900.2043574090874699No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC28290.20004398280569288No Hit
GTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGAAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA27800.19657909939901952No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA27430.19396275886744982No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27410.19382133505493254No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27160.19205353739846653No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26890.19014431592948325No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26290.18590160155396485No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCGTCAACTGCAAGTCCAGC26100.1845580753350507No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25860.18286098958484334No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC25760.18215387052225693No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTG25280.17875969902184222No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23860.1687186083331153No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATTTTATAGTACTCCTGTCACTTT23550.16652653923909747No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22390.15832395811309521No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22330.15789968667554338No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22260.15740470333173287No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22200.15698043189418104No Hit
GTCTCTGGGCGAGAGGGTCACCGTCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAAGAACTA21870.15464693898764592No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21690.1533741246749904No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21630.15294985323743857No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21440.1516063270185244No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATTTTATAGTACTCCTG21400.15132347939348983No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21170.1496971055495411No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTTCTCATTTACTGGGCATCTACCCGGGGATC20920.1479293078930751No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20880.14764646026804057No Hit
CTATAAAATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC20690.1463029340491264No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20440.14453513639266038No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20300.14354516970503944No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20170.1426259149236771No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT19630.13880747198571053No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTTCTCATT19560.13831248864190007No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19510.13795892911060686No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA19340.13675682670420997No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19330.13668611479795134No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19040.13463546951645078No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACAGGAGTA19040.13463546951645078No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19010.13442333379767485No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18930.13385763854760574No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18630.13173628135984652No Hit
TCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCGTCAACTG18600.13152414564107062No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG18590.13145343373481197No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18290.12933207654705275No Hit
GTAAATGAGAAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA17980.12714000745303491No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC17940.12685715982800036No Hit
CTACGGGGACATCGTGATGACCCAATCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCGTCAACTG17890.12650360029670715No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAATCTCCAGACTCC17870.12636217648418988No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17730.12537220979656893No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17660.12487722645275842No Hit
TTATTACTGTCAGCAATTTTATAGTACTCCTGTCACTTTCGGCCCTGGGA17540.12402868357765476No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17470.12353370023384427No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17200.121624478764861No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17110.12098807160853323No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17060.12063451207724003No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT16980.12006881682717091No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16810.11886671442077401No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTTC16310.11533111910784202No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16260.11497755957654882No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16040.11342189763885874No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15760.11144196426361681No Hit
ACCCGGGGATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC15730.11122982854484088No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15720.11115911663858226No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG15670.11080555710728905No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15670.11080555710728905No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15360.1086134880132712No Hit
GCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGACAGG15180.10734067370061569No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15060.10649213082551201No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTTCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTC15060.10649213082551201No Hit
CTTTGGTCCCAGGGCCGAAAGTGACAGGAGTACTATAAAATTGCTGACAGTAATAAACTGCCACATCTTCAGC14960.10578501176292561No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA14900.10536074032537378No Hit
GCTAAGTAGTTCTTATTGTTGGAGTTGTATAAAACACTCTGGCTGGACTT14680.10380507838768369No Hit
CCCAATCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGTCACCGTC14630.10345151885639049No Hit
GGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14560.10295653551258No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14530.10274439979380409No Hit
GCAGAAACCAGGACAGCCTCCTAAGCTTCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATT14520.10267368788754545No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAAGAACTACT14380.10168372119992448No Hit
CATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA14300.10111802594985535No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14300.10111802594985535No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGC14230.10062304260604488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.048.933711
TCGTATG2850.039.86587540
GTGGTAT2400.039.4407421
CTTATAC3100.039.2589871
CGTATGC3000.039.1117841
CCCACGA2950.038.97427419
ACGAGAC3000.038.3382622
TCTCCGA2950.037.82128511
ATGCCGT2950.037.6210344
GACTAGG3100.037.14359726
AGACTAG3100.037.12781525
CTAGGCA3150.036.60977628
ATCTCGT2850.036.0689737
CGAGACT3200.035.95229723
GTATCAA32500.035.3666421
CCGTCTT3100.034.8741747
ATCTCCG3200.034.86649710
GAGACTA3300.034.86530324
CGTCTTC3150.034.39839648
CCGAGCC3250.034.3276614
Produced by FastQC (version 0.11.5)