FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297832
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT74300.5724932040510636No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59000.4546042939301851No Hit
GTTTAGGAGGCTGTCCTGGTTTTTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGTGTATAAAAGACT56960.4388857725807346No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54540.42023929137207283No Hit
CTTTTATACACCTCCAACAATAAGAACTACTTAGCTTGGTACCAACAAAA49290.37978721436981056No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43730.3369465385350338No Hit
GTTTATTACTGTCAGCAAAATTATAGTCCTCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA42690.3289331747098238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40000.30820630096961704No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37250.28701711777795585No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36260.27938901182895787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34740.26767717239211236No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG34030.2622065105499017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33260.2562735392562365No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32840.25303737309605556No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32840.25303737309605556No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31700.2442534935184215No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31420.24209604941163418No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30220.23284986038254568No Hit
GTGTATGGAGGACTATAATTTTGCTGACAGTAATAAACTGACACATCATCAGCCTGCAGGCTACTGATGGTGAGA28890.2226020008753059No Hit
GTAATAAACTGACACATCATCAGCCTGCAGGCTACTGATGGTGAGACTGAAATCTGTCCCAGACCCGCTGCCA28810.22198558827336667No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27950.21535915280251988No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCAC26410.20349321021518965No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25800.19879306412540296No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCATCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACACCTCCAA25690.19794549679773651No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25030.19286009283173783No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCATCATCAACTGCAAGTCCAGT24410.18808289516670879No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24370.18777468886573917No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCATCATCAACTGCAAGTCCAGTCAGAGTCTTTTATA24130.18592545105992148No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23250.1791449124385899No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23180.17860555141189308No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG23120.17814324196043863No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22990.17714157148228737No Hit
GTATAAAAGACTCTGACTGGACTTGCAGTTGATGATGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA22820.1758316947031665No Hit
TCAGTAGCCTGCAGGCTGATGATGTGTCAGTTTATTACTGTCAGCAAAATTATAGTCCTCCATACACTTTCGGCC22450.17298078641919754No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21950.16912820765707734No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA21180.1631952363634122No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTGTTGGTA20920.1611918954071097No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20910.1611148438318673No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20690.15941970917653442No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20660.1591885544508072No Hit
AAATTATAGTCCTCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT20450.1575704713707167No Hit
GATTTCAGTCTCACCATCAGTAGCCTGCAGGCTGATGATGTGTCAGTTTATTACTGTCAGCAAAATTATAGTCCT20310.15649174931732304No Hit
TCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG19540.1505587780236579No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19440.14978826227123387No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC19400.14948005597026426No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19260.14840133391687058No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19080.1470144055625073No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCACCATCAGTAGCC19040.1467061992615377No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT18950.14601273508435605No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18780.1447028583052352No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18770.14462580672999278No Hit
GTCTCTGGGCGAGAGGGCCATCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACACCTCCAACAATAAGAACTA18670.14385529097756874No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18560.1430077236499023No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18310.1410814342688422No Hit
CTACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATC18240.14054207324214538No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17930.13815347440963083No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17770.13692064920575236No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17700.13638128817905554No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17670.13615013345332833No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17380.13391563777129858No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG17360.13376153462081378No Hit
CCTCCAACAATAAGAACTACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGG17300.13329922516935935No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17260.13299101886838974No Hit
GAACTACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGC17170.1322975546912081No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17140.1320663999654809No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17120.13191229681499608No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17080.13160409051402647No Hit
GGCTGATGATGTGTCAGTTTATTACTGTCAGCAAAATTATAGTCCTCCAT16970.13075652318636002No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16860.12990895585869358No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16840.12975485270820877No Hit
CACCATCAGTAGCCTGCAGGCTGATGATGTGTCAGTTTATTACTGTCAGCAAAATTATAGTCCTCCATACACTTT16730.1289072853805423No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16710.1287531822300575No Hit
ACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGCTCATT16670.12844497592908788No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTGTTGGTACCAAGCTA16570.12767446017666384No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAA16430.12659573812327019No Hit
GGTCTGGGACAGATTTCAGTCTCACCATCAGTAGCCTGCAGGCTGATGAT16150.12443829401648286No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16120.12420713929075565No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16110.12413008771551325No Hit
ATCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACACCTCCAACAATAA16090.12397598456502844No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC16010.1233595719630892No Hit
GTCTGGGACAGATTTCAGTCTCACCATCAGTAGCCTGCAGGCTGATGATG15920.12266610778590757No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15880.12235790148493796No Hit
TCATCAGCCTGCAGGCTACTGATGGTGAGACTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC15830.12197264360872594No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15560.11989225107718103No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15480.11927583847524179No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15410.11873647744854496No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15230.11734954909418169No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15190.11704134279321207No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCACCATCAG15170.11688723964272726No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCA14920.11496095026166717No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14870.11457569238545512No Hit
CTATAATTTTGCTGACAGTAATAAACTGACACATCATCAGCCTGCAGGCTACTGATGGTGAGACTGAAATCTGTC14820.1141904345092431No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14800.11403633135875829No Hit
GCACAATACGCCGCGTCTGTGAGAGACAGATTCATTATCTCAAGAGATGA14580.1123411967034254No Hit
GCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTC14560.1121870935529406No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14450.11133952622527415No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCATCATCAACTG14340.11049195889760771No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14140.1089509273927596No Hit
CTCCTGTACAACTTCTGGATTCAGCTTTGGTGATTATGCTATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG14070.10841156636606278No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14030.10810336006509316No Hit
CAATAAGAACTACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTAC13890.10702463801169951No Hit
CCTCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC13880.1069475864364571No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13870.1068705348612147No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13790.10625412225927547No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13790.10625412225927547No Hit
ACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCACCATCAGTAGC13600.10479014232966978No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13570.10455898760394257No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13570.10455898760394257No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC13340.10278680137336728No Hit
GCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGTATGG13220.10186218247045843No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13210.10178513089521603No Hit
GGCCGAAAGTGTATGGAGGACTATAATTTTGCTGACAGTAATAAACTGAC13210.10178513089521603No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13150.1013228214437616No Hit
CTGCAAGTCCAGTCAGAGTCTTTTATACACCTCCAACAATAAGAACTACT13130.1011687182932768No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13110.10101461514279199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTT150.001942890772.4336365
ATCCGAC200.007736739751.0993312
TCGTATG1150.050.98748440
ACGAGAC3350.045.7822
CTTATAC3600.045.433881
CGTATGC1600.045.3338141
CGAGACT3400.045.11198423
ATGCCGT1850.045.0250844
GTGGTAT2450.044.506661
ACTACGC3450.044.49077627
CACGAGA3450.044.4513321
GAGACTA3600.043.5525624
ATCTCCG3650.042.92946210
TATGCCG1950.042.6457943
GTATGCC1950.042.59903742
TACGCTG3650.042.08865429
ATCTCGT1800.041.9957537
GGTATCA8300.041.875811
CCCACGA3750.040.88576519
CGCTGCA3700.040.65845531
Produced by FastQC (version 0.11.5)