FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297832
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT65980.5083862934493832No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58180.44828606476030797No Hit
GTTTAGGAGGCTGTCCTGGTTTTTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGGTGTATAAAAGACT53610.4130734948745292No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47050.362527661515512No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46100.3552077618674836No Hit
CTTTTATACACCTCCAACAATAAGAACTACTTAGCTTGGTACCAACAAAA40400.3112883639793132No Hit
GTTTATTACTGTCAGCAAAATTATAGTCCTCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA40380.3111342608288284No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37190.28655480832650143No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36300.2796972181299275No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35490.2734560405352927No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34190.26343933575378015No Hit
GTAATAAACTGACACATCATCAGCCTGCAGGCTACTGATGGTGAGACTGAAATCTGTCCCAGACCCGCTGCCA34130.2629770263023257No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33820.2605884274698112No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33590.2588162412392359No Hit
GTGTATGGAGGACTATAATTTTGCTGACAGTAATAAACTGACACATCATCAGCCTGCAGGCTACTGATGGTGAGA33270.25635059083147893No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31090.23955334742863485No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29930.23061536470051597No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29900.23038420997478876No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28750.22152327882191225No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27800.21420337917388382No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27330.21058195513749084No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA27120.20896387205740033No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25950.19994883775403902No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25100.19339945385843468No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG24710.1903944424239809No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCAC24600.18954687509631446No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT23700.18261223332449808No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23670.18238107859877087No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCATCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACACCTCCAA23210.1788367061376203No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23050.1776038809337418No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22430.17282668326871273No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCATCATCAACTGCAAGTCCAGTCAGAGTCTTTTATA21690.16712486670077484No Hit
TCATCAGCCTGCAGGCTACTGATGGTGAGACTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC21280.16396575211583628No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20810.16034432807944327No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCATCATCAACTGCAAGTCCAGT20750.15988201862798884No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20710.15957381232701923No Hit
GATTTCAGTCTCACCATCAGTAGCCTGCAGGCTGATGATGTGTCAGTTTATTACTGTCAGCAAAATTATAGTCCT20570.15849509027362554No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20540.15826393554789833No Hit
AAATTATAGTCCTCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT20220.1557982851401414No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA19910.15340968630762686No Hit
TCAGTAGCCTGCAGGCTGATGATGTGTCAGTTTATTACTGTCAGCAAAATTATAGTCCTCCATACACTTTCGGCC19840.15287032528093003No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG19760.1522539126789908No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19710.15186865480277878No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19670.15156044850180916No Hit
GGCTGATGATGTGTCAGTTTATTACTGTCAGCAAAATTATAGTCCTCCAT19630.15125224220083955No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19460.14994236542171868No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19160.14763081816444654No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18290.1409273311183574No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18210.14031091851641816No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTGTTGGTA18190.14015681536593336No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT18010.13876988701157006No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17970.13846168071060044No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17870.1376911649581764No Hit
GTATAAAAGACTCTGACTGGACTTGCAGTTGATGATGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA17810.13722885550672198No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCACCATCAGTAGCC17740.13668949448002515No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17730.13661244290478275No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT17380.13391563777129858No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17320.13345332831984416No Hit
TCCATACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG17300.13329922516935935No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16920.130371265310148No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16920.130371265310148No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16870.12998600743393599No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16790.12936959483199673No Hit
GTCTCTGGGCGAGAGGGCCATCATCAACTGCAAGTCCAGTCAGAGTCTTTTATACACCTCCAACAATAAGAACTA16600.12790561490239105No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16470.1269039444242398No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG16340.12590227394608855No Hit
CTATAATTTTGCTGACAGTAATAAACTGACACATCATCAGCCTGCAGGCTACTGATGGTGAGACTGAAATCTGTC16310.12567111922036134No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16220.12497765504317972No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16030.12351367511357401No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15920.12266610778590757No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15910.12258905621066517No Hit
CTACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAATC15820.12189559203348353No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC15620.12035456052863545No Hit
GGCCGAAAGTGTATGGAGGACTATAATTTTGCTGACAGTAATAAACTGAC15350.11827416799709053No Hit
GTCTGGGACAGATTTCAGTCTCACCATCAGTAGCCTGCAGGCTGATGATG15340.11819711642184813No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15220.11727249751893928No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15200.11711839436845448No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15170.11688723964272726No Hit
GGCTACTGATGGTGAGACTGAAATCTGTCCCAGACCCGCTGCCACTGAAT14820.1141904345092431No Hit
CTCCTGTACAACTTCTGGATTCAGCTTTGGTGATTATGCTATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG14770.11380517663303108No Hit
GGTCTGGGACAGATTTCAGTCTCACCATCAGTAGCCTGCAGGCTGATGAT14670.11303466088060704No Hit
ACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGCTCATT14610.11257235142915262No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14460.11141657780051656No Hit
CACCATCAGTAGCCTGCAGGCTGATGATGTGTCAGTTTATTACTGTCAGCAAAATTATAGTCCTCCATACACTTT14450.11133952622527415No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14420.11110837149954694No Hit
AACCAGGACAGCCTCCTAAACTGCTCATTTACTGGGCATCTACCCGGGAA14390.11087721677381973No Hit
CCTCCAACAATAAGAACTACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGCTCATTTACTGGG14370.11072311362333492No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14340.11049195889760771No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14300.1101837525966381No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14200.10941323684421403No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14140.1089509273927596No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14140.1089509273927596No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCAGTCTCACCATCAG14000.10787220533936595No Hit
GTAAATGAGCAGTTTAGGAGGCTGTCCTGGTTTTTGTTGGTACCAAGCTA13790.10625412225927547No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13650.10517540020588181No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC13600.10479014232966978No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13560.10448193602870016No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13500.10401962657724574No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13490.10394257500200334No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC13470.10378847185151853No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13470.10378847185151853No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCA13430.10348026555054891No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13420.10340321397530651No Hit
GAACTACTTAGCTTGGTACCAACAAAAACCAGGACAGCCTCCTAAACTGC13110.10101461514279199No Hit
GCACAATACGCCGCGTCTGTGAGAGACAGATTCATTATCTCAAGAGATGA13100.10093756356754958No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13070.10070640884182237No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC13000.10016704781512555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAA200.004410197558.89933468
GGTATCA9050.042.4781721
TATTGGT503.7630161E-641.52707742
GTGGTAT3100.038.409821
TGGTATC3100.038.3979762
ACAGGTA1450.037.5394481
GTATCAA20550.035.5928341
GACAGCT2300.035.4992641
GAATACG751.8702012E-631.7349859
ATACGTA751.8702012E-631.73498511
TTCTGCG450.004234757331.2212352
GCATAAC450.004950708730.2401121
TATCAAC24250.030.1528722
TACGTAC803.096342E-629.74811212
ATCAACG25100.029.3970873
TCAACGC25200.029.142134
CAACGCA25450.028.9905725
AACGCAG25550.028.8771046
ATATAAC500.006405447628.68724362
AGGTATC605.8962824E-428.3413642
Produced by FastQC (version 0.11.5)