FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690852
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGCAAGATG28460.41195509313137985No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACAC24290.3515948423106541No Hit
GGATTACCTGTGGTGGATACAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC23300.33726471082084153No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22360.3236583233456659No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20820.30136700769484637No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20680.2993405244538628No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20570.2977482876216614No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTA18600.2692327734449636No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16770.24274374250924943No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC15400.22291315650819565No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTT14330.2074250345949639No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14230.20597754656568992No Hit
GTTTTACTTCCAATGTTGTATCCACCACAGGTAATCCTGGCCATCTTGCCTGGGGCCACTGACACCGAGGGTGG14080.2038063145217789No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13940.2017798312807953No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT13840.20033234325152133No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC13710.1984506088134651No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13700.1983058600105377No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA13510.1955556327549171No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13110.18976568063782112No Hit
GGATAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC12960.18759444859391014No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12940.18730495098805533No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12940.18730495098805533No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG12870.18629170936756353No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT12810.18542321654999913No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12610.18252824049145114No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT12440.18006751084168535No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12400.17948851562997575No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGCAAGATGGCCAGGATTAC12160.17601454435971814No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12100.17514605154215376No Hit
GTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTA12010.17384331231580716No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11960.17311956830117015No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11890.17210632668067835No Hit
GGGTGGCCGTGTTCGCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA11800.17080358745433175No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11740.16993509463676734No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG11720.16964559703091256No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11660.16877710421334816No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11560.16732961618407416No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGCGACTGAAGGTGATTCCAGAGGCGGCACA11520.16675062097236457No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11470.16602687695772755No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG11040.15980267843184937No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC10950.15849993920550276No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC10910.15792094399379317No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC10730.15531546554109998No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10490.15184149427084237No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10450.15126249905913278No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGCTATTCGGCG10450.15126249905913278No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG10320.14938076462107658No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA10150.1469200349713108No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG10100.1461962909566738No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG10010.1448935517303272No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT9940.14388031010983537No Hit
TAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCC9800.1418538268688518No Hit
AAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGG9780.141564329262997No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9760.1412748316571422No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9720.1406958364454326No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC9460.1369323675693202No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCA9430.136498121160538No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA9340.13519538193419142No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9290.1344716379195544No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9280.134326889116627No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGG8960.12969492742295022No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8820.1276684441819666No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8540.12361547769999942No Hit
GCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC8340.12072050164145143No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGCAAGATGG8310.12028625523266923No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTTCCAATGTTGTATCCACCACAGGTAATCCTGGC8290.11999675762681443No Hit
CCCTCGGTGTCAGTGGCCCCAGGCAAGATGGCCAGGATTACCTGTGGTGGATACAACATTGGAAGTAAAACTGTG8200.11869401840046782No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8120.11753602797704864No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTAC8070.11681228396241163No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8030.11623328875070202No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8000.11579904234191984No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7870.11391730790386363No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7850.11362781029800884No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7850.11362781029800884No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGGCCACC7830.11333831269215403No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7830.11333831269215403No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGCAAGATGGCCAG7700.11145657825409783No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGC7570.10957484381604166No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC7520.10885109980140464No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7490.10841685339262244No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7450.10783785818091285No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7430.10754836057505805No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7360.10653511895456626No Hit
GTGTCAGTGGCCCCAGGCAAGATGGCCAGGATTACCTGTGGTGGATACAA7290.10552187733407445No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7260.10508763092529223No Hit
GGATACAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGG7220.10450863571358265No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7110.10291639888138125No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7080.10248215247259905No Hit
GTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCAT7060.10219265486674425No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7020.10161365965503466No Hit
GGGATCCCTGAGGGCCGGTCGCTATCATCATAGACGACCACCACAGGGGC7020.10161365965503466No Hit
CAGGTAATCCTGGCCATCTTGCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTCACAGAG7010.10146891085210726No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6950.10060041803454285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAT150.002119903570.8551860
TAACGGA200.006556279553.28138461
ACCGTTA200.00668012153.0299159
GATTGGC200.007655395751.23358521
AGGGGGG1251.5097612E-1049.46349770
GTGGTAT1600.044.826141
ACGAGAC1300.044.6651822
GCTAATC1250.043.88131333
TGGTATC1650.043.4677772
TATACAC1500.043.2607883
GGTATCA5400.042.375331
CCCACGA1500.040.98390619
CGAGACT1450.040.04754623
CACGAGA1450.040.04464721
CGTATGC1050.039.50114441
TACACAT1650.039.327995
ATGTGAT1601.7043931E-938.64335670
GTATCAA14000.038.5443881
AGCTAAT1600.038.5367632
TCTCGTA901.2551027E-1038.26655638
Produced by FastQC (version 0.11.5)